Pichia Protocols (Methods in Molecular Biology)

David R. Higgins; James M. Cregg

ISBN 10: 0896034216 / ISBN 13: 9780896034211

Editore: Humana Press, 1998

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Ex- library copy with stamps and stickers. Codice articolo mon0003287687

Dati bibliografici

Titolo: Pichia Protocols (Methods in Molecular ...

Casa editrice: Humana Press

Data di pubblicazione: 1998

Legatura: hardcover

Condizione: Good

Descrizione articolo

Riassunto:

Contains the techniques, tools, and sample applications needed to successfully use the Pichia pastoris expression system. Step-by-step instructions allow beginners to utilize the system and supply to experienced researchers time-saving techniques, ideas, and information. Sixteen chapters contains detailed protocols from the construction of expression vectors to the production of foreign proteins in high-density fermentor cultures and, in addition, in-depth descriptions of selected protein expression stories. Annotation c. by Book News, Inc., Portland, Or.

Dalla quarta di copertina:

The Pichia pastoris expression system has rapidly become one of the key protein expression systems used in commercial, academic, and government laboratories today. In Pichia Protocols, David Higgins and James Cregg compile for the first time an all-inclusive collection of powerful experimental procedures to manipulate Pichia pastoris for gene expression purposes. The methods-developed by world-class researchers in their own laboratories-contain detailed descriptions of available expression strains, as well as vectors, and step-by-step instructions for the construction of expression strains and for their use in the production of foreign proteins from shake-flask or fermentor cultures. General procedures for the purification of foreign proteins are also provided, along with protocols for analyzing foreign protein products for glycosylation and subcellular location.

Pichia Protocols captures the collective expertise of the international P. pastoris scientific community in close detail. It conveys a breadth of experience that will permit any researcher in molecular biology to quickly construct an expression strain designed to produce a desired foreign protein, and to use this state-of-the-art molecular and cellular biological system successfully in every area of biological research today.