This book deals with historical perspectives and current status of doubled haploid production, along with its practical implications. It highlights various methods with a comprehensive discussion on their pros and cons, bottlenecks, and embryogenic pathways.
Abstract.- Introduction/Historical background.- Haploid Production.- Androgenesis.- Microspore culture.- Donor plant’s growth and developmental conditions.- Collection of floral organs.- Pretreatments.- Microspore’s isolation and purification.- Media Composition.- Regeneration.- Increase in Ploidy Level.- Albinism.- Pathways of microspore embryogenesis.- Anther Culture.- Genotype, physiological state, growth and developmental stage of donor plants.- Pretreatments and media composition.- Uniparental Chromosome Removal/Elimination or Wide Hybridization.- Bulbosum Method.- Haploids using maize as a pollen donor.- Haploids using Solanum phureja and maize inducer lines.- Gynogenesis.- Genotype.- Developmental stage of female gametophyte.- Pretreatment.- Composition of media.- Parthenogenesis.- Applications and uses of haploids.- Homozygosity.- Genomics.- Mutation.- Transformation.- Synthetic or Artificial Seed Production.- Future Thrust.- Conclusion.- References.