Protein Arrays : Methods and Protocols (Methods in Molecular Biology Volume 264)

Eric T. Fung

ISBN 10: 158829255X / ISBN 13: 9781588292551

Editore: Humana, USA, 2004

Riguardo questo articolo

158829255X. Pictorial board hardcover, from closed pharmaceutical company library. Lending record shows this book has been borrowed once only. 287 pages, appendix, refs, index, illustrated with charts, tables and diagrams. Methods to synthesize and construct protein arrays for basic and clinical research in a high-throughput manner. These reproducible techniques can be applied to such applications as quantifying specific proteins of interest and discovering novel proteins, as well as to a variety of affinity substances, including antibodies, peptides, proteins, aptamers, and chemicals. The techniques can be used for biomarkers discovery, assay development, clinical sample testing, and building the next generation of molecular tools. Contents clean, tight and bright.

Codice articolo 027347

Dati bibliografici

• Titolo: Protein Arrays : Methods and Protocols (Methods in Molecular Biology Volume 264)
• Editore: Humana, USA
• Data di pubblicazione: 2004
• Lingua: Inglese
• ISBN 10: 158829255X
• ISBN 13: 9781588292551
• Rilegatura: Hardcover
• Condizione: Fine
• Tipo di libro: Book
• Cataloghi dei venditori:
  • Biochemistry

Descrizione articolo

Riassunto

Protein Arrays: Methods and Protocols is an introduction to protein array technology and its application to the multiplexed detection of proteins. Although protein array technology has some roots in gene array technology, it can only be described as a distant relative. Unlike DNA, with its established rules of base pairing, and therefore predictable biochemical behavior, proteins are rich with diversity. Proteins can be large or small, compact or extended, basic or acidic, hydrophobic or hydrophilic, and so on. Just as importantly, their behavior is determined by the environment in which they reside, and so the composition of the buffer in which experiments are performed has a dramatic impact on the outcome of the experiment. Thus, if the goal is to simultaneously measure the expression of a large number of proteins, these variables must be addressed. Not to be deterred, scientists have created a variety of solutions to successfully detect and ch- acterize multiple proteins simultaneously. It is the intent of this volume to introduce to the reader a set of technological solutions to the diversity problem as well as to provide the reader with some examples of practical applications of these technologies.

Dalla quarta di copertina

Protein arrays make possible the detection and quantitation of many proteins simultaneously, thus enabling researchers to ask fundamental questions about biological processes and to discover biomarkers that can be used diagnostically. In Protein Arrays: Methods and Protocols, innovative experimentalists describe in detail the methods they have developed to synthesize and construct protein arrays for basic and clinical research. The authors present protocols to create and immobilize the capture substrate-the first task in designing a protein array-using a variety of affinity capture reagents, including antibodies, peptides, aptamers, biotin, chemical reagents, and chromatographic substrates. Once synthesized, these arrays can be used to analyze protein-protein interactions and posttranslational modifications, such as phosphorylation, as well as to discover and characterize potential diagnostic markers. Protocols to accomplish these tasks are also presented. The protocols presented follow the successful Methods in Molecular Biology series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Diverse and highly practical, Protein Arrays: Methods and Protocols offers basic and clinical investigators a broad spectrum of approaches to the generation of protein arrays, as well as their uses in biomarkers discovery, in assay development, in clinical sample testing, in signal transduction analysis and characterization, and for creating the next generation of molecular tools.