Protein-Dye Interactions: Developments and Applications

This volume contains the papers and reports presented at the First International Conference on Dye-Protein Interaction, held 24-28 July 1988 at the University of Compiegne, France. This was the first international meeting dealing entirely with dye-protein interaction. The major focus of the conference was on the better understanding of the mechanism of interaction of proteins with different triazine dyes and the synthesis of novel structural dyes having good biomimetic activities. The potentials and limits of their use in biotechnology, mainly for purification, were stressed. Current contributions in developing dye-based affinity methods were highlighted in such areas as affinity partition, affinity precipitation and new support matrices for efficient affinity chromatography, etc. The interrelation between metal chelates and dyes in terms of their interactions with proteins was underlined. It is our belief that this proceedings volume will be a stimulus for broad and creative applications of dye affinity concepts in many fields of biomedical research and biotechnology. for understanding the In addition, a discussion session emphasised the necessity toxicological aspects of these dyes, their fragments and their metabolites. This helped to trigger plans for future work, and this topic will be one of the priorities in a future meeting on dye-protein interactions. C. R. The help of the International Scientific Committee, which included Drs Lowe (UK), G. KopperschHiger (GDR) , E. Stellwagen (USA), D. Thomas (France), G. Birkenmeier (GDR), S. Rajgopal-Narayan (USA), J. P. Dandeu (France), D. Muller (France) and E. Dellacherie (France), in organising this meeting is gratefully acknowledged.

Introduction: A Short Historical Review on the Interaction Between Dye-stuffs and Biological Matter.- Introduction: A Short Historical Review on the Interaction Between Dye-stuffs and Biological Matter.- 1: Dye Structures and their Relevance to Protein Recognition.- Biomimetic Dyes in Biotechnology.- Structure, Preparation and Chemistry of Reactive Dyes.- Dye Fragments in Protein—Dye Interactions.- 2: Physico-chemical Aspects of Dye—Protein Interactions.- Reactive Dyes as a Spectral Probe for Protein Folding.- Ionic and Apolar Interactions of Cibacron Blue F3GA with Model Compounds and Proteins: Application to Protein Purification.- A Quantitative Study of the Interaction of Lactate Dehydrogenase and Immobilised Cibacron Blue F3GA in the Absence of Soluble Ligands.- Interaction of Azo Dyes with Cationic Polypeptides: Poly-l-Arginine—4-Dimethylamino Azobenzene-4’—Carboxylic Acid System.- Kinetic and Thermodynamic of the Interaction of Dyes with Biological Macromolecules.- 3: Dye—Ligand Adsorbents in Chromatographic Systems.- The Use of Biospecific Elution in Purifying Proteins from Dye—Ligand Adsorbents.- The Influence of Matrices on Dye—Protein Interaction.- Evaluation of Chromatography Supports Prepared by Grafting Reactive Dyes onto Dextran Coated Silica Beads.- Fast Analytical Dye—Ligand Chromatography in Process Development.- A Comparison of Macrosorb—Cibacron Blue under Fixed-Bed, Batch Adsorption and Fluidised-Bed Conditions.- Polymer Design in Dye Chromatography: From the Definition of Monomers to the Evaluation of Polymeric Supports.- The Use of Dyes in Affinity Ligand and Protein Immobilization.- 4: Dye Ligands in Affinity Partition, Affinity Ultrafiltration and Affinity Precipitation.- Dye—Ligand Affinity Partitioning—a Powerful Method for Studying Enzyme—Dye Interaction.- Affinity Partition and Solubilisation of Enzymes by Use of Polymer-Bound Triazine Dyes.- The Comparative Study of the Interaction of Yeast and Horse Liver Alcohol Dehydrogenases with Reactive Dyes by Affinity Partitioning in Two-Phase Systems.- Affinity Ultrafiltration and Affinity Precipitation Using a Water-Soluble Complex of Poly(Vinyl Alcohol) and Cibacron Blue F3GA.- Affinity Precipitation of (NAD Dependent Dehydrogenase) Lactate Dehydrogenase Using Cibacron Blue Dimers.- 5: Role of Added Ions in Dye—Protein Interactions.- Metal Enhanced Interactions of Proteins with Triazine Dyes and Applications to Downstream Processing.- Metal Mediated Modulation of Enzyme Kinetics by Structurally Related Dyes.- Study of the Interaction of NAD(H)-Dependent Dehydrogenases with Reactive Dyes and their Complexes with Transition Metal Ions.- Immunoglobulin and Prealbumin Interactions with Remazol Yellow GGL: Prealbumin Requires an Anion.- 6: Blood Proteins Purification Using Dye—Ligand Affinity.- Dye Serum Protein Interaction—Analysis and Application.- Recovery of Albumin from Cohn Fraction IV Using Immobilised Procion Blue-HB: Development of a Full Scale Production Process.- The Interaction of Human ?-Fetoprotein with Reactive Dyes and the Isolation of the Protein by Affinity Chromatography.- 7: Dye—Ligand Affinity Chromatography for the Purification of Plant Proteins.- Downstream Processing of Potato Proteins.- A New Procedure for Purification of Pop Corn Inhibitor Based on Immobilized Dye Chromatography.- 8: Dyes in Molecular Biology.- Influence of Dye Binding on the Integrity and Function of Nucleic Acids and Nucleoproteins: Complexation of Polysomes with Coomassie Blue.- Progressive Unfolding of the Conformational States of Transfer RNA and Ribosomal 5S RNA by Methylene Blue Binding.- Use of Triazine Dyes in Purification of T4 Polynucleotide Kinase.- Epilogue.- Index of Contributors.