Riassunto:
A readily reproducible collection of established and emerging techniques for studying the interaction between proteins and ligands, including biochemical/bulk techniques, structure analysis, spectroscopy, single-molecule studies, and theoretical/computational tools. Among the highlights are surface plasmon resonance (SPR) and reflectometric biosensor approaches, high-throughput screening with confocal optics microscopy, single molecule fluorescence and fluorescence correlation spectroscopy (FCS), atomic force microscopy (AFM), crystallography of reaction intermediates, and time-resolved x-ray crystallography. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Contenuti:
Isothermal Titration Calorimetry Edwin A. Lewis and Kenneth P. Murphy Direct Optical Detection of Protein-Ligand Interactions Frank Gesellchen, Bastian Zimmermann, and Friedrich W. Herberg Label-Free Detection of Protein-Ligand Interactions by the Quartz Crystal Microbalance Andreas Janshoff and Claudia Steinem Measurement of Solvent Accessibility at Protein-Protein Interfaces Jeffrey G. Mandell, Abel Baerga-Ortiz, Arnold M. Falick, and Elizabeth A. Komives Hydrophobic Interaction Chromatography: Harnessing Multivalent Protein-Surface Interactions for Purification Procedures Herbert P. Jennissen Sedimentation Velocity Method in the Analytical Ultracentrifuge for the Study of Protein-Protein Interactions Claus Urbanke, Gregor Witte, and Ute Curth Protein-Ligand Interaction Probed by Time-Resolved Crystallography Marius Schmidt, Hyotcherl Ihee, Reinhard Pahl, and Vukica Srajer X-Ray Crystallography of Protein-Ligand Interactions Ilme Schlichting Combined Use of XAFS and Crystallography for Studying Protein-Ligand Interactions in Metalloproteins Richard W. Strange and S. Samar Hasnain NMR Studies of Protein-Ligand Interactions Till Maurer Probing Heme Protein-Ligand Interactions by UV/Visible Absorption Spectroscopy Karin Nienhaus and G. Ulrich Nienhaus Ultrafast Time-Resolved IR Studies of Protein-Ligand Interactions Manho Lim and Philip A. Anfinrud Monitoring Protein-Ligand Interactions by Time-Resolved FTIR Difference Spectroscopy Carsten Kötting and Klaus Gerwert Proteins in Motion: Resonance Raman Spectroscopy as a Probe of Functional Intermediates Uri Samuni and Joel M. Friedman Fluorescence Polarization/Anisotropy Approaches to Study Protein-Ligand Interactions: Effects of Errors and Uncertainties David M. Jameson and GaborMocz Ligand Binding With Stopped-Flow Rapid Mixing Mark S. Hargrove Circular Dichroism Spectroscopy for the Study of Protein-Ligand Interactions Alison Rodger, Rachel Marrington, David Roper, and Stuart Windsor High-Throughput Screening of Interactions Between G Protein-Coupled Receptors and Ligands Using Confocal Optics Microscopy Lenka Zemanová, Andreas Schenk, Martin J. Valler, G. Ulrich Nienhaus, and Ralf Heilker Single-Molecule Study of Protein-Protein and Protein-DNA Interaction Dynamics H. Peter Lu Application of Fluorescence Correlation Spectroscopy to Hapten-Antibody Binding Theodore L. Hazlett, Qiaoqiao Ruan, and Sergey Y. Tetin Atomic Force Microscopy Measurements of Protein-Ligand Interactions on Living Cells Robert H. Eibl and Vincent T. Moy Computer Simulation of Protein-Ligand Interactions: Challenges and Applications Sergio A. Hassan, Luis Gracia, Geetha Vasudevan, and Peter J. Steinbach Force Probe Molecular Dynamics Simulations Helmut Grubmüller Study of Ligand-Protein Interactions by Means of Density Functional Theory and First-Principles Molecular Dynamics Carme Rovira Index
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