Protocols for Nucleic Acid Analysis by Nonradioactive Probes (Methods in Molecular Biology)

Isaac, Peter G., ed

ISBN 10: 089603254X / ISBN 13: 9780896032545

Editore: Humana Press, Totowa, N.J, 1994

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Ex- library book with stamps/ stickers. Codice articolo mon0003288151

Dati bibliografici

Titolo: Protocols for Nucleic Acid Analysis by ...

Casa editrice: Humana Press, Totowa, N.J

Data di pubblicazione: 1994

Legatura: paperback

Condizione: Good

Descrizione articolo

Riassunto:

This book provides an exceptionally useful collection of tried and tested protocols for nucleic acid analysis that avoid the use of radioisotopes. Southern (i.e., DNA) and Northern (i.e., RNA) blotting protocols are covered in detail, from how to isolate good quality nucleic acids from plant and animal sources, to the use of the widest range of probe systems (involving digoxigenin, biotin, fluorescein, horseradish peroxidase labeling, and colorimetric and chemiluminescent detection).
Other techniques treated in detail include hybridization in situ to chromosomes and transcribed RNA, newer and less conventional technologies, such as Randomly Amplified Polymorphic DNA (RAPD), reverse dot blots, bacterial identification using magnetic head and dipstick assays, and for the first time in detail, Nucleic Acid Sequence-Based Amplification.

Recensione:

...this book provides a collection of good protocols which are in general clearly written. They can be easily employed on the bench without being a specialist in a particular fields. - FEBS Letters

...Covers the basics of a variety of probe-labelling systems very well...a very interesting read. It could save you money and trouble from the radiation safety officer.-Biotechnology and Applied Biochemistry