Pyrosequencing Protocols (Methods in Molecular Biology, 373)

ISBN 10: 1617376930 ISBN 13: 9781617376931

Editore: Humana, 2010

Lingua: Inglese

Condizione: Nuovo Brossura

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Dati bibliografici

Titolo

Pyrosequencing Protocols (Methods in Molecular Biology, 373)

Editore

Humana

Data di pubblicazione

2010

Lingua

Inglese

ISBN 10

1617376930

ISBN 13

9781617376931

Rilegatura

Brossura

Condizione

New

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Descrizione articolo

Riassunto

Pyrosequencing� Protocols presents detailed protocols for the multidisciplinary application of Pyrosequencing� technology, all written by world-renowned experts in the field. This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing�, and supplementing each protocol with troubleshooting tips specific to that method.

Chapters 1 and 2 present an introductory overview of the origins of the Pyrosequencing� methodology and its key applications. Chapters 3 and 4 describe primer selection and the basic Pyrosequencing� technique, and Chapters 5-7 provide methods for improving throughput and decreasing cost. Detailed applications for Pyrosequencing� comprise Chapters 8-13, and the important aspects of data storage is discussed in Chapter 14. This comprehensive volume both highlights the versatility of and provides detailed protocols for the application of Pyrosequencing�.

Contenuti

Table of contents Chapter 1: The History of Pyrosequencing�. P�l Nyr�n Chapter 2: Pyrosequencing� applications. Sharon Marsh Chapter 3: Web-Based Primer Design Software for Genome Scale Genotyping by Pyrosequencing�. Steven Ringquist, Christopher Pecoraro, Ying Lu, Alexis Styche, William A. Rudert, Panayiotis V. Benos and Massimo Trucco Chapter 4: Pyrosequencing�: A Simple Method for Accurate Genotyping. Cristi R. King and Tiffany Scott-Horton Chapter 5: Universal Primer Applications for Pyrosequencing�. Dong-chuan Guo and Dianna M. Milewicz Chapter 6: Allele Quantification and DNA Pooling Methods. Jonathon Wasson Chapter 7: Multiplex Pyrosequencing� for DNA Variation Analysis. Pritesh Patel, Yih-Horng Shiao, and Paolo Fortina Chapter 8: Analysis of gene-specific DNA methylation patterns by the Pyrosequencing� technology. J�rg Tost and Ivo Glynne Gut Chapter 9: Detecting mutations that confer oxazolidinone resistance in Gram-positive bacteria. Neil Woodford, Sarah E. North and Matthew J. Ellington Chapter 10: Pyrosequencing� Based Strategies for Improved Allele Typing of HLA Loci. Steven Ringquist, Alexis Styche, William A. Rudert, and Massimo Trucco Chapter 11: Pyrosequencing� of Phage Display Libraries for the Identification of Cell Specific Targeting Ligands. Ahad A. Rahim Chapter 12: Gene Copy Number Detection in Animal Studies. Gerli Pielberg and Leif Andersson Chapter 13: Detection of Allelic Imbalance in Gene Expression Using Pyrosequencing�. Hua Wang and Steven C. Elbein Chapter 14: Pyrosequencing� Genotype Storage Techniques. Derek J. Van Booven

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