Rapid Cycle Real-Time PCR: Methods and Applications

Dietmaier, Wolfgang; Wittwer, Carl; Sivasubramanian, Natarajan

ISBN 10: 3540426000 / ISBN 13: 9783540426004

Editore: Springer, Berlin, Germany, 2002

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VERY GOOD+ hardcover, no marks in text, tight binding, name inside front board, very clean exterior.

Codice articolo 076421

Dati bibliografici

• Titolo: Rapid Cycle Real-Time PCR: Methods and Applications
• Editore: Springer, Berlin, Germany
• Data di pubblicazione: 2002
• Lingua: Inglese
• ISBN 10: 3540426000
• ISBN 13: 9783540426004
• Rilegatura: Hardcover
• Condizione: Very Good
• Tipo di libro: Book
• Cataloghi dei venditori:
  • Alibris

Descrizione articolo

Riassunto

Rapid-Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green I often allows product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization probes, melting curves provide "dynamic dot blots" for fine sequence analysis, including single nucleotide polymorphisms (SNPs). The method is often cited as the most versatile, efficient method for nucleic acid analysis in research and diagnostics in the fields of genetics and oncology. Molecular diagnostics has never been easier!

Dalla quarta di copertina

Rapid Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green I allow product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization probes, melting curves provide "dynamic dot blots" for fine sequence analysis, including single nucleotide polymorphisms. The method is often cited as the most versatile, efficient method for nucleic acid analysis in research and analysis in the fields of Genetics and Oncology. Molecular analysis has never been easier!