Rapid Cycle Real-Time Pcr - Methods and Applications: Genetics and Oncology

ISBN 10: 3540426000 / ISBN 13: 9783540426004

Editore: Springer-Verlag Berlin and Heidelberg GmbH & Co. K, 2002

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Edition 2002. Ammareal reverse jusqu'à 15% du prix net de cet article à des organisations caritatives. ENGLISH DESCRIPTION Book Condition: Used, As new. Edition 2002. Ammareal gives back up to 15% of this item's net price to charity organizations.

Codice articolo E-952-042

Dati bibliografici

• Titolo: Rapid Cycle Real-Time Pcr - Methods and Applications: Genetics and Oncology
• Editore: Springer-Verlag Berlin and Heidelberg GmbH & Co. K
• Data di pubblicazione: 2002
• Lingua: Inglese
• ISBN 10: 3540426000
• ISBN 13: 9783540426004
• Rilegatura: Hardcover
• Condizione: Comme neuf

Descrizione articolo

Riassunto

Rapid-Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green I often allows product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization probes, melting curves provide "dynamic dot blots" for fine sequence analysis, including single nucleotide polymorphisms (SNPs). The method is often cited as the most versatile, efficient method for nucleic acid analysis in research and diagnostics in the fields of genetics and oncology. Molecular diagnostics has never been easier!

Dalla quarta di copertina

Rapid Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green I allow product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization probes, melting curves provide "dynamic dot blots" for fine sequence analysis, including single nucleotide polymorphisms. The method is often cited as the most versatile, efficient method for nucleic acid analysis in research and analysis in the fields of Genetics and Oncology. Molecular analysis has never been easier!