Real-Time Analysis of Biomolecular Interactions: Applications of BIACORE

Kazuhiro Nagata

ISBN 10: 443170289X / ISBN 13: 9784431702894

Editore: Springer, 2000

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Codice articolo G443170289XI4N00

Dati bibliografici

• Titolo: Real-Time Analysis of Biomolecular Interactions: Applications of BIACORE
• Editore: Springer
• Data di pubblicazione: 2000
• Lingua: Inglese
• ISBN 10: 443170289X
• ISBN 13: 9784431702894
• Rilegatura: Hardcover
• Condizione: Very Good
• Condizione della sovraccoperta: No Jacket

Descrizione articolo

Riassunto

The metabolism of all living organisms consists of elaborate and complex interactions among numbers of biomolecules, including protein-protein interactions. BIACORE is a device that uses surface plasmon resonance for kinetic analysis of molecular interactions and automates whole analytical processes. The number of users of BIACORE is increasing worldwide, but until now nothing had been published in English to explain the principles of this new methodology and the kinds of applications it makes available. This updated and revised version of the Japanese original explains the underlying principles of BIACORE, providing concrete examples that utilize the methodology for analysis in areas ranging from basic to applied sciences.

Contenuti

(1) Significance of the Real-Time Analysis of Biological Interactions.- (2) General Principles: Principles of Surface Plasmon Resonance. Principles of BIACORE.- (3) Basic Procedures: Experimental Procedures. Data Analysis Methods.- (4) Applications: Protein-Protein Interactions. Peptid-Protein Interactions. DNA-Protein Interactions. DNA-DNA Interactions. Sugar-Protein Interactions. Lipid-Protein Interactions. Applications for Purification and Screening. Clinical Applications. Combinations with Other Methods.- (5) The View for the Future.