Subcellular Fractionation: A Practical Approach

Graham, J. M.; Rickwood, D. (eds.)

ISBN 10: 0199634947 / ISBN 13: 9780199634941

Editore: IRL Press, Oxford, 1997

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Paperback. Minor mark on page block; interior appears clean throughout. All content is clear. TS.

Codice articolo 607333

Dati bibliografici

• Titolo: Subcellular Fractionation: A Practical Approach
• Editore: IRL Press, Oxford
• Data di pubblicazione: 1997
• Lingua: Inglese
• ISBN 10: 0199634947
• ISBN 13: 9780199634941
• Rilegatura: Paperback
• Condizione: Very Good
• Tipo di libro: Used
• Cataloghi dei venditori:
  • Science - Genetics/Biology/Evolution

Descrizione articolo

Riassunto

Many investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers all the necessary aspects, from breaking up the cells (homogenization), via a variety of separation techniques (the isolation and fractionation chapters), to characterization of the separated organelles.

Contenuti

• 1.: Homogenization of tissues and cells
• 2.: Isolation of subcellular fragments
• 3.: Isolation and characterization of nuclei and nuclear subfractions
• 4.: Subcellular fractionation of mitochondria
• 5.: Isolation and characterization of peroxisomes
• 6.: Lysosomes and endocytosis
• 7.: The membranes of the secretory and exocytic pathways
• 8.: Isolation and purification of functionally intact mitochondria and chloroplasts from plant cells
• 9.: Ribosomes and polysomes
• 10.: Electron microscopy of organelles