Synthetic Messenger RNA and Cell Metabolism Modulation

. Ed(s): Rabinovich, Peter M.

ISBN 10: 1627032592 / ISBN 13: 9781627032599

Editore: Humana Press Inc., 2013

This Methods in Molecular Biology(TM) book covers mRNA synthesis, modifications and delivery, offering protocols, material lists and practical tips along with examples of cell reprogramming and analysis in the fields of immunotherapy and stem cell research. Editor(s): Rabinovich, Peter M. Series: Methods in Molecular Biology. Num Pages: 323 pages, 3 black & white tables, biography. BIC Classification: MFN. Category: (P) Professional & Vocational. Dimension: 254 x 178 x 23. Weight in Grams: 826. . 2012. Hardback. . . . . Books ship from the US and Ireland. Codice articolo V9781627032599

Riassunto:

Synthetic mRNA is an attractive tool for mammalian cell reprogramming that can be used in basic research, as well as in clinical applications. Present mRNA in vitro synthesis is a rather simple procedure, which delivers a high yield of quality product. Various modifications may be introduced into the mRNA by changing the sequence of the DNA template, by modifying the reaction of transcription, or by post-transcriptional modification. mRNA, as a transfection agent, has several advantages over DNA, as mRNA expression is not dependent on nuclear entry and occurs directly in the cytosol. Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols covers the typical main methods, such as mRNA synthesis, modifications, and delivery. Examples of cell reprogramming and analysis in the fields of immunotherapy and stem cell research are also included. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols will be of interest to researchers, clinicians, and biotech companies interested in mRNA-mediated cell reprogramming.

Dalla quarta di copertina:

Synthetic mRNA is an attractive tool for mammalian cell reprogramming that can be used in basic research, as well as in clinical applications. Present mRNA in vitro synthesis is a rather simple procedure, which delivers a high yield of quality product. Various modifications may be introduced into the mRNA by changing the sequence of the DNA template, by modifying the reaction of transcription, or by post-transcriptional modification. mRNA, as a transfection agent, has several advantages over DNA, as mRNA expression is not dependent on nuclear entry and occurs directly in the cytosol. Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols covers the typical main methods, such as mRNA synthesis, modifications, and delivery. Examples of cell reprogramming and analysis in the fields of immunotherapy and stem cell research are also included. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols will be of interest to researchers, clinicians, and biotech companies interested in mRNA-mediated cell reprogramming.

Dati bibliografici

Titolo: Synthetic Messenger RNA and Cell Metabolism ...
Casa editrice: Humana Press Inc.
Data di pubblicazione: 2013
Legatura: Rilegato
Condizione: New