TGF-? Signaling: Methods and Protocols (Methods in Molecular Biology (1344))

ISBN 10: 1493949934 / ISBN 13: 9781493949939

Editore: Humana Press, 2016

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pp. 406.

Codice articolo 26382658254

Dati bibliografici

• Titolo: TGF-? Signaling: Methods and Protocols (Methods in Molecular Biology (1344))
• Editore: Humana Press
• Data di pubblicazione: 2016
• Lingua: Inglese
• ISBN 10: 1493949934
• ISBN 13: 9781493949939
• Rilegatura: Brossura
• Condizione: New

Descrizione articolo

Riassunto

This volume details up-to-date information and attempts to take the reader into the exciting realm of TGF-β from the basic principles to the practical applications. Chapters provide basic introduction of TGF-β signaling from the cell surface to the nucleus, methods and techniques for the investigation of TGF-β signaling mechanism including receptors, Smads, intracellular kinases, microRNA, epigenetic regulation, post-translational regulations, non-Smad pathway; the physiological implications including those in cell cycle arrest, epithelial-mesenchymal transition, endothelial cells, adipogenesis, Th differentiation, stem cell, bone remodeling, ovary, zebrofish development, and frog animal capping; and the methodologies including metastasis imaging, 3D morphogenesis, membrane receptor quantification, conditional knockout, bone remodeling, kinase and phosphatase assays, BiFC interaction assays, and genome-wide siRNA screen. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, TGF-Beta Signaling: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Dalla quarta di copertina

This volume details up-to-date information and attempts to take the reader into the exciting realm of TGF-ß from the basic principles to the practical applications. Chapters provide basic introduction of TGF-ß signaling from the cell surface to the nucleus, methods and techniques for the investigation of TGF-ß signaling mechanism including receptors, Smads, intracellular kinases, microRNA, epigenetic regulation, post-translational regulations, non-Smad pathway; the physiological implications including those in cell cycle arrest, epithelial-mesenchymal transition, endothelial cells, adipogenesis, Th differentiation, stem cell, bone remodeling, ovary, zebrofish development, and frog animal capping; and the methodologies including metastasis imaging, 3D morphogenesis, membrane receptor quantification, conditional knockout, bone remodeling, kinase and phosphatase assays, BiFC interaction assays, and genome-wide siRNA screen. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, TGF-Beta Signaling: Methods and Protocols aims to ensure successful results in the further study of this vital field.