Transgenesis Techniques: Principles and Protocols (Methods in Molecular Biology)

CLARKE ALAN R.

ISBN 10: 0896036960 / ISBN 13: 9780896036963

Editore: Humana Press, 2002

Well packaged and promptly shipped from California. Partnered with Friends of the Library since 2010. Codice articolo 1LAUHV0021UT

Riassunto:

This revised and updated edition of a much-acclaimed classic presents a collection of readily reproducible techniques for the generation and analysis of transgenic animals, with emphasis on the manipulation of the mammalian genome. It concentrates on the creation and maintenance of genetically modified murine strains, providing access to the germline by both conventional pronuclear injection, and by retroviral and adenoviral infection. Extensive coverage is also given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for both constitutive and conditional (Cre-lox or the Flp-frt systems) gene targeting. Additional chapters provide cutting-edge techniques for the cryopreservation of both male and female germlines, for the generation of transgenic sheep by nuclear transfer, and for the basic analysis of transgenic organisms.

Dalla quarta di copertina: The past decade has witnessed a spectacular explosion in both the development and the use of transgenic technologies. In Transgenesis Techniques: Principles and Protocols, Second Edition, authoritative researchers-including members of the Dolly team-take stock of these exciting developments to present a collection of readily reproducible techniques for the generation and analysis of transgenic animals, focusing on the manipulation of the mammalian genome. This second edition devotes much space to the creation of genetically modified murine strains, providing access to the germline both by conventional pronuclear injection and by retroviral and adenoviral infection. Extensive coverage is also given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for both constitutive and conditional (Cre-lox or the Flp-frt systems) gene targeting. Additional chapters provide cutting-edge techniques for the cryopreservation of both male and female germlines, for the generation of transgenic sheep by nuclear transfer, and for the basic analysis of transgenic organisms.
Comprehensive and highly practical, this second edition of Transgenesis Techniques: Principles and Protocols updates and expands the acclaimed first edition with a wealth of easy-to-follow cutting-edge protocols and practical tips for producing transgenic animals and analyzing their integrated transgenes.

Dati bibliografici

Titolo: Transgenesis Techniques: Principles and ...
Casa editrice: Humana Press
Data di pubblicazione: 2002
Legatura: Rilegato
Condizione: New
Edizione: seconda edizione