Trypanothione Synthetase and Trypanothione Reductase from L. donovani

Equbal, Asif/ Zaidi, Amir/ Ali, Vahab

ISBN 10: 333035318X / ISBN 13: 9783330353183

Editore: LAP LAMBERT Academic Publishing, 2017

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264 pages. 8.66x5.91x0.60 inches. In Stock.

Codice articolo 333035318X

Dati bibliografici

• Titolo: Trypanothione Synthetase and Trypanothione Reductase from L. donovani
• Editore: LAP LAMBERT Academic Publishing
• Data di pubblicazione: 2017
• Lingua: Inglese
• ISBN 10: 333035318X
• ISBN 13: 9783330353183
• Rilegatura: Paperback
• Condizione: Brand New
• Peso: 0,44 chilogrammi

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Riassunto

Visceral Leishmaniasis is a severe form of disease that is nearly always fatal in the majority of untreated cases. In the parasite the apparent dependency of synthesis and regulation of trypanothione on thiol metabolic pathway, the role of TryS and TryR in drug resistance and redox homeostasis and its interaction has remained unexplored. So, in this study, we have characterized TryS and TryR proteins of L. donovani, investigate potential interaction between them and tried to identify their correlation with oxidative stress and drug resistance. The enzymatic activities and kinetic properties of both enzymes were also studied. We elucidate for the first time the interaction between TryR and TryS in L. donovani by in silico analysis coupled with in vitro and in vivo interaction which suggests a stable complex formation between TryR & TryS in L. donovani. Further, we observed that LdTryS and LdTryR significantly upregulated at both transcriptional and translational level in Amp B resistant isolates and during transformation from proliferative logarithmic growth phase to infective stationary phase, thus help the parasite for the survival in the macrophage.