Type 3 Secretion Systems

. Ed(s): Nilles, Matthew L.; Condry, Danielle

ISBN 10: 1493966472 / ISBN 13: 9781493966479

Editore: Humana Press Inc., 2016

Editor(s): Nilles, Matthew L.; Condry, Danielle. Series: Methods in Molecular Biology. Num Pages: 224 pages, 9 black & white illustrations, 23 colour illustrations, 16 colour tables, biography. BIC Classification: PSD; PSG. Category: (P) Professional & Vocational. Dimension: 254 x 178 x 14. Weight in Grams: 645. . 2016. Hardback. . . . . Books ship from the US and Ireland. Codice articolo V9781493966479

Riassunto:

This volume discusses various basic and advanced methods and protocols that have been proven to be successful among certain bacterial species, or a family of species, in type III secretion systems (T3S system). The chapters in this book cover topics such as: site-directed mutagenesis and its application in studying the interactions of T3S components; use of transcriptional control to increase expression and secretion of heterologous proteins in T3S systems; fractionation techniques to examine effector translocation; detecting immune responses to T3S systems; mouse immunization with purified needle proteins from T3S systems and the characterization of the immune responses to these proteins; and detection of protein interactions in T3S systems using yeast 2-hybrid analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and comprehensive, Type 3 Secretion Systems: Methods and Protocols is a valuable resource for anyone interested in learning about the fascinating and ever-changing T3S systems.

Dalla quarta di copertina:

This volume discusses various basic and advanced methods and protocols that have been proven to be successful among certain bacterial species, or a family of species, in type III secretion systems (T3S system). The chapters in this book cover topics such as: site-directed mutagenesis and its application in studying the interactions of T3S components; use of transcriptional control to increase expression and secretion of heterologous proteins in T3S systems; fractionation techniques to examine effector translocation; detecting immune responses to T3S systems; mouse immunization with purified needle proteins from T3S systems and the characterization of the immune responses to these proteins; and detection of protein interactions in T3S systems using yeast 2-hybrid analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Practical and comprehensive, Type 3 Secretion Systems: Methods and Protocols is a valuable resource for anyone interested in learning about the fascinating and ever-changing T3S systems.

Dati bibliografici

Titolo: Type 3 Secretion Systems
Casa editrice: Humana Press Inc.
Data di pubblicazione: 2016
Legatura: Rilegato
Condizione: New