VEGF Signaling: Methods and Protocols (Methods in Molecular Biology)

ISBN 10: 149392916X / ISBN 13: 9781493929160

Editore: Humana Press, 2015

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pp. 223.

Codice articolo 26372381351

Dati bibliografici

• Titolo: VEGF Signaling: Methods and Protocols (Methods in Molecular Biology)
• Editore: Humana Press
• Data di pubblicazione: 2015
• Lingua: Inglese
• ISBN 10: 149392916X
• ISBN 13: 9781493929160
• Rilegatura: Rilegato
• Condizione: New

Descrizione articolo

Riassunto

This volume provides a collection of protocols for studying and manipulating VEGF signaling pathways in vitro and in vivo, and in particular, aims to present a range of both firmly established and newly emerging technologies, including those that are amenable to aiding in drug discovery or pre-clinical investigations. Each of the three sections begins with an introductory overview followed by supporting relevant methods. A major advantage of this book is that each chapter not only contains methodological detail rarely seen in other literature, but also contains a section on key notes and troubleshooting advice. Part I focuses on quantification of specific VEGF and VEGF receptor isoforms, beginning with a summary on VEGF splice variants, their function and their regulation. Part II relates to the study of VEGF dependent signaling in vitro, starting with a comprehensive overview of VEGF-mediated signal transduction in the cardiovascular system that highlights recent discoveries in the field. Part III first details genetic mouse models, then discusses techniques for studying physiological angiogenesis in the developing mouse embryo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, VEFG Signaling: Methods and Protocols is a useful tool for researching and understanding the basic biology of VEGF signaling and translating it into the clinic.

Informazioni sull?autore

Lorna R. Fielder, Program in cardiovascular and Metabolic Disorders, Duke-NUS, Graduate Medical School Singapore, 8 College Road, Singapore 169857, Telephone: +65-6516-7666, Email: lorna.fiedler@gmail.com or l.fiedler@imperial.ac.uk.