Yeast Genetics: Methods and Protocols is a collection of methods to best study and manipulate Saccharomyces cerevisiae, a truly genetic powerhouse. The simple nature of a single cell eukaryotic organism, the relative ease of manipulating its genome and the ability to interchangeably exist in both haploid and diploid states have always made it an attractive model organism. Genes can be deleted, mutated, engineered and tagged at will. Saccharomyces cerevisiae has played a major role in the elucidation of multiple conserved cellular processes including MAP kinase signaling, splicing, transcription and many others. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Yeast Genetics: Methods and Protocols will provide a balanced blend of classic and more modern genetic methods relevant to a wide range of research areas and should be widely used as a reference in yeast labs.
1. Yeast Transformation by LiAc/SS Carrier DNA/PEG Method
R. Daniel Gietz
2. Tetrad, Random Spore and Molecular Analysis of Meiotic Segregation and Recombination
Michael Lichten
3. PCR Mutagenesis and Gap Repair in Yeast
Mark Weir and Jill B. Keeney
4. PCR-Mediated Epitope Tagging of Genes in Yeast
Radhika Mathur and Peter Kaiser
5. Manipulating the Yeast Genome: Deletion, Mutation and Tagging by PCR
Jennifer M. Gardner and Sue L. Jaspersen
6. Preparation of Yeast Cells for Live Cell Imaging and Indirect Immunofluorescence
Lucy F. Pemberton
7. Single Yeast Cell Imaging
Heimo Wolinski and Sepp D. Kohlwein
8. Microfluidic Platforms for Generating Dynamic Environmental Perturbations to Study the Responses of Single Yeast Cells
Anjali Bisaria, Pascal Hersen, and Megan N. McClean
9. Using Two-Hybrid Interactions to Identify Separation-of-Function Mutations
Brian Haarer and David C. Amberg
10. Synthetic Genetic Array Analysis of Global Mapping of Genetic Networks in Yeast
Elena Kuzmin, Sara Sharifpoor, Anastasia Baryshnikova, Michael Costanzo, Chad L. Myers, Brenda J. Andrews, and Charles Boone
11. Chemical Genetic and Chemogenomic Analysis in Yeast
Namal V. C. Coorey, Liam D.P. Sampson, Jacqueline M. Barber, and David S. Bellows
12. Phenomic Assessment of Genetic Buffering by Kinetic Analysis of Cell Arrays
John Rodgers, Jingyu Guo, and John L. Hartman, IV
13. Detection of Short-Range Chromatin Interactions by Chromosome Conformation Capture (3C) in Yeast
Badri Nath Singh and Michael Hampsey
14. Chromosome Conformation Capture (3C) of Tandem Arrays in Yeast
Maria D. Mayán and Luis Aragón
15. Global Analysis of Transcription Factor Binding Sites in Yeast Using ChIP-Seq
Philippe Lefrançois, Jennifer E.G. Gallagher, and Michael Snyder
16. High-Density Tiling Microarrays Analysis of the Full Transcriptional Activity of Yeast
David Lior, Sandra Clauder-Münster, and Lars M. Steinmetz
17. Analysis of Silencing in Saccharomyces cerevisiae
Andrew Miller and Ann L. Kirchmaier
18. A User’s Guide to the Ribosomal DANN in Saccharomysces cerevisiae
Joseph M. Johnson, Jeffrey S. Smith, and David A. Schneider
19. Two-Dimensional Agarose Gel Electrophoresis for Analysis of DNA Replication
Sandra K. Villwock and Oscar M. Aparicio
20. Replicative Life Span Alaysis in Budding Yeast
George L. Sutphin, Joe R. Delaney, and Matt Kaeberlein
21. Metabolomic and Lipidomic Analyses of Chronologically Aging Yeast
Vincent R. Richard, Simon D. Bourque, and Vladimir I. Titorenko
