mRNA Decay: Methods and Protocols (Methods in Molecular Biology, 1720)

ISBN 10: 1493975390 / ISBN 13: 9781493975396

Editore: Humana Press, 2017

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Codice articolo 18376471707

Dati bibliografici

• Titolo: mRNA Decay: Methods and Protocols (Methods in Molecular Biology, 1720)
• Editore: Humana Press
• Data di pubblicazione: 2017
• Lingua: Inglese
• ISBN 10: 1493975390
• ISBN 13: 9781493975396
• Rilegatura: Rilegato
• Condizione: New

Descrizione articolo

Riassunto

This detailed volume explores the latest methods that can be used to probe mRNA decay pathways and identify mRNA-binding protein targets as well as miRNA targets. Subjects include metabolic labelling and RNAseq methods for determining RNA decay rates, approaches for discovering RNA-binding protein targets, bioinformatics, miRNA targets and novel components of the miRNA-directed decay pathway, and recently developed approaches for studying nonsense-mediated mRNA decay, among other areas. Written for the highly popular Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, mRNA Decay: Methods and Protocols serves as an ideal guide for molecular biologists, geneticists, and developmental biologists with an interest in understanding how normal development and tissue homeostasis is regulated and how these processes are perturbed in inherited and acquired diseases.