New York: Humana Press, 2019. Very Good condition but for notes on the front endpapers, and notes and a small soiled spot on the next two pages. All other pages are clean and unmarked with NO underlining. NO highlighting. NO margin notes. With many illustrations, some in color. Molecular diagrams. Tables. Bibliographical references. Index. Volume # 1961 in the publisher's Methods in Molecular Biology series. For no apparent reason, someone has drawn a harmless circle on the front cover (2 inches in diameter). From the rear cover: "This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPR gene editing field.". First Edition. Hardcover. Very Good condition/No dust jacket, as issued. Illus. by NOT a library discard. xi, 362pp. Great Packaging, Fast Shipping.

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Dati bibliografici

Titolo

CRISPR Gene Editing: Methods and Protocols

Editore

Humana Press, New York

Data di pubblicazione

2019

Lingua

Inglese

ISBN 10

1493991698

ISBN 13

9781493991693

Rilegatura

Hardcover

Disegnatore

NOT a library discard

Edizione

First Edition.

Condizione

Very Good condition

Condizione della sovraccoperta

No dust jacket, as issued

Cataloghi dei venditori

Science / Technology / Computers

Descrizione articolo

Riassunto

This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPR gene editing field.

Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.

Dalla quarta di copertina

Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.

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