Near Fine or Very Good+ condition without a dust jacket. The covers are in great shape with only a hint of wear to the spine ends. The binding is square and tight. The interior pages are clean and unmarked. The book will be carefully packaged for shipment for protection from the elements. Abrasion to end papers. USPS electronic tracking number issued free of charge.

Codice articolo 13749

Contatta il venditore

Segnala questo articolo

Dati bibliografici

Titolo: Chemical Mutagens: Principles and Methods for Their Detection: Volume 2
Editore: Springer, New York
Lingua: Inglese
ISBN 10: 0306371022
ISBN 13: 9780306371028
Rilegatura: Hardcover
Edizione: First Edition.
Stampa: First Printing
Condizione: Very Good
Condizione della sovraccoperta: No Jacket
Dimensioni: 9,25 pollici di larghezza per 6,15 pollici di altezza per 1 pollici di profondit�

Descrizione articolo

Riassunto

The best protection against environmental mutagens is to identify them before they ever come into general use. But it is always possible that some substance will escape detection and affect a large number of persons without this being realized until later generations. This article considers ways in which such a genetic emergency might be promptly detected. A mutation-detecting system should be relevant in that it tests for effects that are as closely related as possible to those that are feared. It should be sensitive enough to detect a moderate increase in mutation rate, able to discover the increase promptly before more damage is done, responsive to various kinds of mutational events, and designed in such a way as to maxi� mize the probability that the Gause of an increase can be found. Methods based on germinal mutation necessarily involve enormous numbers of persons and tests. On the other hand, with somatic mutations the individual cell becomes the unit of measurement rather than the in� dividual person. For this reason, I think that somatic tests are preferable to germinal tests, despite the fact that it is germinal mutations which are feared.

Contenuti

of Volume 2.- 11 Measurement of Recessive Lethal Damage Over the Entire Genome and at Two Specific Loci in the ad-3 Region of a Two-Component Heterokaryon of Neurospora Crassa.- I. Introduction.- A. Use of a Two-Component Heterokaryon to Measure the Genetic Effects of Mutagenic Treatment.- B. The Spectrum of Recessive Lethal Mutations Detectable with a Two-Component Heterokaryon.- C. Characteristics of Strain 12-A Two-Component Heterokary on.- D. Assaying for Mutagenicity of Chemicals in Screening Programs.- II. Measurement of the Genetic Effects of Mutagenic Treatment.- A. General Methods of Treatment.- B. Tests for Mutagenicity on Growing Cultures.- C. Tests for Mutagenicity on Nongrowing Conidia.- D. Treatment with Mutagens.- E. Assaying for the Genetic Effects of Mutagenic Treatment.- F. Evaluation of Genetic Assays.- III. Characterization of Presumptive ad-3 Mutants.- A. Establishing a Silica Gel Stock Culture of Each Adenine-Requiring Strain.- B. Genetic Tests.- IV. Media and Chemical Solutions.- A. Media.- B. Chemical Solutions.- V. References.- 12 Aspergillus.- I. Introduction.- II. Life Cycle and Genetic Analysis.- A. The Vegetative Cycle.- B. The Sexual Cycle.- C. The Parasexual Cycle.- III. Mutation.- A. Gene Mutations.- B. Mutations of Quantity and Arrangement.- IV. Conclusions.- V. References.- 13 Higher Plants.- I. Introduction.- II. Test Systems.- A. Barley.- B. Pea.- C. Wheat and Other Polyploids.- D. Arabidopsis thaliana.- III. Compounds Tested for Mutagenic Activity by Treatment of Seeds.- A. Classification of Mutagens.- B. Quantitative Aspects.- C. Alkylating Agents.- D. Potential Unspecific Mutagens Active by Mechanisms Other Than Alkylation.- E. Agents Causing Specific Changes in DNA Bases.- IV. Special Techniques That Have a Higher Resolving Power.- A. Pollen Characters.- B. Somatic Mutant Sectors.- V. General Evaluation.- VI. Acknowledgments.- VII. References.- 14 Procedures for Culturing Diploid Cells and Preparation of Meiotic Chromosomes from Dwarf Species of Hamsters.- I. Introduction.- II. Background Information.- A. Brief Historical Account.- B. Breeding and Management.- C. General Characteristics of Animals and Cultured Cells.- D. Cytological Preparations.- E. Spontaneous Tumors.- III. Handling the Diploid Cell.- A. Cloning Operations.- B. Difficulties When Employing Single Type Serum Supplemented Media.- C. Cell Culture Media for Routine Propagation of Different Diploid Cell Types.- D. Combined Sera Supplements.- E. Phenotypic Features of Diploid Cells.- F. Initiation of Cell Cultures from Organs and Solid (Primary) Tumors.- G. Derivation of Diploid Cell Lines from Peritoneal Exudate.- H. Roller-Flask Cultures of Diploid Cell Lines.- IV. Spermatogonial and Meiotic Chromosome Preparations.- A. Partial Orchidectomy.- B. Hypotonic Treatment and Fixation.- C. Aceto-Carmine Squash Preparations.- D. Propiono-Carmine Squash Preparation.- E. Air-Dried Preparations.- F. Construction of a Low-Cost Centrifuge Tube “Flicker”.- G. Preparation of Lactic-Acetic-Orcein.- H. Permanent Slides.- V. Bone Marrow Biopsy Procedure.- VI. Discussion.- VII. Acknowledgments.- VIII. References.- 15 Induction and Analysis of Gene Mutations in Mammalian Cells in Culture.- I. Introduction.- II. The Cell Material.- A. Species Origin.- B. Karyotypic Stability.- C. Plating or Cloning Efficiency.- D. Cold Storage of Cells.- III. Utilization of In Vivo Markers.- A. Morphological Markers.- B. Biochemical Markers.- C. Serological Markers.- D. Radiation-Sensitive Mutants.- IV. Detection of Recessive Mutations in Cell Cultures.- A. Heterozygosity of Autosomal Recessive Genes.- B. Aneuploidy for Autosomes.- C. Natural Monosomy of X Chromosomes in Normal Males or in XO Females.- D. Functional Monosomy of X Chromosomes in Normal Females.- E. Independent Mutation at Both Alleles in Homozygous Dominant Genes.- V. Selective Techniques for New Mutations in Cell Culture.- A. Mass Selection Method.- B. Lethal-Growth Method.- C. “Thymineless Death” Method.- D. Replica-Plating Method.- VI. Characterization of Newly Isolated Variants.- VII. Procedure for Mutation Induction.- A. Choice of the Test System.- B. Forward and Back Mutations.- C. Determination of Cytotoxicity.- D. Elimination of Background Mutations.- E. Treatment with Mutagen.- F. Inoculation of Cells.- G. Addition of Selective Agent.- H. Isolation and Testing of Mutant Colonies.- I. Fixation and Staining of Colonies.- J. Mutation Rate and Mutation Frequency.- VIII. Concluding Remarks.- IX. Acknowledgments.- X. References.- 16 Inducing Mutations with Chemicals in Habrobracon.- I. Introduction.- II. Maintenance of Habrobracon and Ephestia.- A. Habrobracon Culture.- B. Ephestia Culture.- III. Scheme for Mutational Analysis.- A. General Stock.- B. Pretest.- C. Collection of Virgins and Males for Experiment.- D. Treatments.- E. Counts.- F. Test of F1 Females.- G. Mating and Further Testing of Females with Genetic Alterations.- H. Special Mutant Analysis.- I. Homozygosity of Mutants.- J. Further Testing.- K. Data Processing.- L. Catalog of Mutants.- IV. Application of Mutagens.- A. Aerosols.- B. Feeding.- C. Topical Application.- D. Microinjection.- V. Conclusions.- VI. References.- 17 The Detection of Mutations in Drosophila melanogaster.- I. Introduction.- II. Advantages of Drosophila as a Test Organism.- III. Limitations of Drosophila as a Test Organism.- IV. Lethal Tests.- A. Sex-Linked Recessive Lethals.- B. Autosomal Recessive Lethal Test.- V. Tests for Recessive Visible Mutants.- VI. Tests for Chromosomal Rearrangements.- A. Genetic Test for Reciprocal Translocation.- B. Position-Effect Tests for Chromosomal Rearrangement.- C. Cytological Test for Chromosomal Rearrangement.- VII. Tests for Loss of X or Y Chromosomes.- VIII. Tests for Dominant Lethals.- IX. Tests for Half-Translocations.- X. Staging of Germ Cells.- XI. Techniques for Collecting Flies of a Desired Sex.- XII. Review of Literature.- XIII. Summary.- XIV. References.- 18 Root Tips for Studying the Effects of Chemicals on Chromosomes.- I. The Material.- A. Root Tips as Experimental Material.- B. The Horse Bean, Vicia faba.- C. The Common Onion, Allium cepa.- D. The Tree Onion, Allium proliferum.- II. Treatment of Root Tips with Chemicals.- III. Fixation and Staining of Root Tips.- IV. Scoring of Slides and Types of Aberration.- V. Comparison Between the Effects of Chemicals on Chromosomes in Root-Tip Cells and in Cultured Animal Cells.- A. Are Results Obtained in Root Tips Representative for Other Materials?.- B. Tepa and Related Compounds.- C. Nitrilotriacetic Acid, NTA.- D. Caffeine.- E. Concluding Remarks.- VI. Acknowledgments.- VII. References.- VIII. Suggested Reading.- 19 Cytogenetic Studies in Animals.- I. Introduction.- II. Experimental Design.- A. Controls.- B. Replication.- C. Observer Bias.- D. Standardizing Scoring Methods.- E. Statistical Evaluation.- F. Types of Damage.- III. Classification of Chromosomal Aberrations.- IV. Localization of Chromosomal Aberrations.- V. Timing of Chromosomal Damage.- VI. Meiotic Studies.- VII. In Vivo and in Vitro Studies.- VIII. Specific Techniques.- A. Lymphocyte Culture.- B. Bone Marrow (Direct).- C. Fibroblast Culture.- D. Amniotic Fluid Cell Culture.- E. Meiotic Preparations.- IX. Summary.- X. References.- 20 Specific Locus Mutation in Mice.- I. Introduction.- II. The Method, Its Advantages and Disadvantages.- III. Results Obtained.- IV. Conclusions.- V. References.- 21 Dominant Lethal Mutations in Mammals.- I. Introduction.- II. The Dominant Lethal Syndrome.- A. Critical Stages of Pregnancy.- B. Modes of Egg or Fetal Death.- III. The Estimation of Dominant Lethals.- A. Mid-Term Litters.- B. Full-Term Litters.- C. Recommended Protocols.- IV. The Genotypes of Dominant Lethals.- A. Chromosome Loss.- B. Monosomy and Trisomy.- Y. Dominant Lethals in Male Germ Cells.- A. Sperm Maturation.- B. Differential Sensitivity.- VI. Dominant Lethals in Female Germ Cells.- VII. General Validity of the Dominant Lethal Test.- VIII. Synergistic Effects.- IX. Review of Chemicals Tested as Dominant Lethal Mutagens in Mammals.- X. Integration of the Dominant Lethal Assay and Other Mutagenicity Tests into General Toxicological Practice.- XI. References.- 22 The Host-Mediated Assay, a Practical Procedure for Evaluating Potential Mutagenic Agents in Mammals.- I. Introduction.- II. Materials and Methods.- A. Strains.- B. Host-Mediated Assay.- III. Results.- A. Salmonella.- B. Neurospora.- C. Comparative Mutagenicity.- IV. Conclusion.- V. References.- 23 Human Population Monitoring.- I. Introduction.- II. A Classification of Mutational Effects.- A. Cytogenetic Changes.- B. Dominant Mutations.- C. X-Linked Recessive Mutations.- D. Autosomal Recessive Mutations.- E. Mutants with Minor Effects.- III. Criteria for a Mutation-Monitoring System.- A. Is the System Relevant?.- B. How Quickly Will a Mutation Increase Be Detected?.- C. Can the System Detect a Small Increase in the Mutation Rate?.- D. Can Many Kinds of Mutational Events Be Detected?.- E. Does the System Offer a High Probability of Identifying the Cause of the Mutation Increase?.- F. Is the System Available Now?.- IV. Some Ways of Amplifying the Mutation-Detecting Power of Monitoring Systems.- A. Somatic Cytogenetic Studies.- B. Somatic Mutation-Detection Systems.- C. Indirect Monitoring by Testing for Mutagens in Human Blood.- V. Monitoring for Germinal Mutations.- A. Monitoring for Dominant Mutant Phenotypes.- B. Biochemical Monitoring.- VI. Summary.- VII. References.- Conclusion.- Author Index.

Le informazioni nella sezione "Su questo libro" possono far riferimento a edizioni diverse di questo titolo.

Descrizione libreria

Alien Bindings is an online bookshop located in Baltimore that has been in operation for nearly 20 years. Alien Bindings caters to the sciences including the physical sciences, astronomy, mathematics, computer science, the social sciences and medicine. I also dabble in a wide array of other categories including antiquarian books and history. I run a clean, green business. I try to reuse clean materials for packaging whenever possible. For example, books are carefully packaged frequently using clean cardboard for custom made boxes, recycled paper is used for printed labels and recycled plastic or bubble wrap is used for a weather-proof barrier. I drop the packages off to my local post office via foot or cycle in order to further reduce the carbon footprint.

Visita la pagina della libreria

Informazioni sull'azienda del venditore

Alien Bindings
MD, U.S.A.

Condizioni di vendita e spedizione

Condizioni di vendita

Diritto di recesso

Se sei un consumatore puoi recedere dal contratto in conformit� con quanto segue. Per Consumatore si intende qualsiasi persona fisica che agisce per scopi estranei alla propria attivit� commerciale, imprenditoriale, artigianale o professionale.

Informazioni sul diritto di recesso

Diritto legale di recesso

Hai il diritto di recedere dal presente contratto entro 14 giorni senza fornire alcuna motivazione.

Il periodo di recesso scade dopo 14 giorni dal giorno in cui tu o una terza parte, diversa dal vettore e da te indicata, acquisisce il possesso fisico dell'ultimo bene o dell'ultimo lotto o pezzo.

Per esercitare il diritto di recesso, compila e invia elettronicamente una dichiarazione esplicita sul nostro sito Web, alla voce “I miei acquisti” nella sezione “Mio account”. Ti comunicheremo senza indugio una conferma di ricezione di tale recesso su un supporto durevole (ad es. via e-mail).

Per rispettare il termine di recesso, � sufficiente inviare la comunicazione relativa all'esercizio del diritto di recesso prima della scadenza del periodo di recesso stesso.

Effetti del recesso

In caso di recesso dal presente contratto, ti rimborseremo tutti i pagamenti ricevuti, compresi i costi di spedizione (ad eccezione dei costi supplementari derivanti dalla tua eventuale scelta di un tipo di spedizione diverso dal tipo meno costoso di consegna standard da noi offerto).

Potremo effettuare una detrazione dal rimborso per la perdita di valore dei beni forniti, qualora tale perdita sia il risultato di una manipolazione non necessaria da parte tua.

Eseguiremo il rimborso senza indebito ritardo e non oltre 14 giorni dal giorno in cui saremo informati della tua decisione di recedere dal presente contratto.

Il rimborso sar� effettuato utilizzando lo stesso mezzo di pagamento da te usato per la transazione iniziale, salvo che tu non abbia espressamente concordato altrimenti; in ogni caso, non dovrai sostenere alcun costo quale conseguenza di tale rimborso.

Possiamo trattenere il rimborso finch� non avremo ricevuto i beni oppure finch� non avrai fornito la prova di averli rispediti, a seconda di quale condizione si verifichi per prima.

Dovrai rispedire i beni o consegnarli a Alien Bindings, BALTIMORE, Maryland, U.S.A., +1 (443)635-6391, senza indebito ritardo e, in ogni caso, entro 14 giorni dal giorno in cui ci hai comunicato la tua volont� di recedere dal presente contratto. Il termine � rispettato se rispedisci i beni prima della scadenza del periodo di 14 giorni. I costi diretti della restituzione dei beni saranno a tuo carico. Sei responsabile solo della diminuzione del valore dei beni risultante da una manipolazione diversa da quella necessaria per stabilire la natura, le caratteristiche e il funzionamento dei beni stessi.

Eccezioni al diritto di recesso

Il diritto di recesso non si applica a:

La fornitura di giornali, periodici o riviste ad eccezione dei contratti di abbonamento; e
La fornitura di contenuto digitale non fornito su un supporto materiale (ad es. su un CD o DVD), se al momento dell'invio dell'ordine hai accettato l'inizio dell'esecuzione e hai riconosciuto che non avresti potuto recedere una volta iniziata l'esecuzione.

Condizioni di spedizione

Tariffe di spedizione nazionale per U.S.A.

Tariffe di spedizione nazionale per U.S.A.
Quantit� dell'ordine	Da 4 a 14 giorni lavorativi	Da 3 a 6 giorni lavorativi
Primo articolo	EUR 0.00	EUR 8.59

I tempi di consegna sono stabiliti dai venditori e variano in base al corriere e al paese. Gli ordini che devono attraversare una dogana possono subire ritardi e spetta agli acquirenti pagare eventuali tariffe o dazi associati. I venditori possono contattarti in merito ad addebiti aggiuntivi dovuti a eventuali maggiorazioni dei costi di spedizione dei tuoi articoli.