Methods for Affinity-Based Separations of Enzymes and Proteins (Paperback)

Munishwar N. Gupta

ISBN 10: 3764363053 / ISBN 13: 9783764363055

Editore: Birkhauser Verlag AG, Basel, 2002

Riguardo questo articolo

Paperback. Extensive troubleshooting guide Big concerns of biotechnology are either using enzymes or producing them. Enzyme/protein production is therefore an important starting point for biotechnology. Bioseparation or Downstream Processing constitutes about 40-90 percent of the total production cost. Driven by economics, highly selective technologies applicable to large-scale processing have emerged during the last decade. These technologies are slowly diffusing to enzymologists who are working on a smaller scale, looking for fast and efficient purification protocols. The affinity-based techniques described in this volume provide current and new cutting-edge methods. Consequently, the book is of main interest to researchers in biochemistry, biochemical engineering and biotechnology, working either in academic or industrial sectors. As each chapter includes a succinct introduction, the volume is a handy and instructive reference also for students majoring in these disciplines. The special focus plus the most detailed protocols of up-to-date methods make this book a very useful and unique reference. One major concern of biotechnology is either using enzymes or producing them. This book covers the affinity-based techniques (including precipitation, two-phase extractions, expanded bed chromatography, perfusion chromatography and monoliths). Shipping may be from multiple locations in the US or from the UK, depending on stock availability.

Codice articolo 9783764363055

Dati bibliografici

• Titolo: Methods for Affinity-Based Separations of Enzymes and Proteins (Paperback)
• Editore: Birkhauser Verlag AG, Basel
• Data di pubblicazione: 2002
• Lingua: Inglese
• ISBN 10: 3764363053
• ISBN 13: 9783764363055
• Rilegatura: Paperback
• Edizione: prima edizione
• Condizione: new

Descrizione articolo

Riassunto

Shape your heart to front the hour, but dream not that the hour will lost. Alfred Tennyson Locksley Hall Sixty YeO!, After Numerous advances in the life sciences have necessitated the need to develop gentle, quick and economical methods for separation of proteins/enzymes. Affmity-based separations, with their high selectivity, have thus come to be used more frequently. In the beginning, we just had atrmity chromatography. Today we have several strategies that employ atrmity interactions for separa­ tion and analysis. This book is an attempt to provide a short introduction and illustrative protocols for some important and current affinity-based approaches inthe area ofdownstream processingofenzymes/proteins. Today, people from a variety of disciplines have to purify proteins. It is hoped that this book will provide some quick insight into the various available options and guide even a novice (in the area ofprotein separation) to obtain his/her target protein at the level of purity that he/she desires.

Contenuti

1 Affinity-Based Separation: An Overview.- 2 Affinity Chromatography.- 3 Expanded Bed Affinity Chromatography.- 4 Perfusion Affinity Chromatography for Rapid Antibody Separations.- 5 Affinity-Based Interactions on Disks for Fast Analysis, Isolation and Conversion of Biomolecules.- 6 Sugars as Affinity Ligands.- 7 Macroaffinity Ligands in Bioseparation.- 8 Cellulose-Binding Fusion Proteins.- 9 Two-Phase Affinity Partitioning of Animal Cells: Implications of Multipoint Interactions.- 10 Affinity-Based Reverse Micellar Separations.- 11 Biosensors: An Emerging Tool in Bioanalytical Research -A Kinetic Analysis of the Analyte-Receptor Interactions in Biosensors.- Guide to Solutions.- Guide to Enzymes.- Guide to Protocols.- Troubleshooting Guide.