Mitochondrial DNA - Methods and Protocols (Methods in Molecular Biology Volume 197)

Copeland, William C., ed.

ISBN 10: 0896039722 / ISBN 13: 9780896039728

Editore: Humana Press, New Jersey, 2002

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Hardcover textbook - clean and unmarked. A very good copy with minor edge-wear and some bumps at the corners. Binding and hinges are solid. 414 pp., indexed, some photos, graphs and charts. Codice articolo 929

Dati bibliografici

Titolo: Mitochondrial DNA - Methods and Protocols (...

Casa editrice: Humana Press, New Jersey

Data di pubblicazione: 2002

Legatura: Hardcover

Condizione: Very Good

Edizione: First edition.

Descrizione articolo

Riassunto:

Internationally recognized authorities describe in great detail the methods they have perfected to analyze mtDNA and the proteins involved in its maintenance. The analytical techniques cover the purification of mtDNA from a variety of sources and the analysis of DNA for both deletions, point mutations, and damage, for replication intermediates, and for following the fate of mtDNA outside of the mitochondria. Additional analytical methods are presented for analyzing the proteins and enzymes that maintain mtDNA. Comprehensive and timely, Mitochondrial DNA: Methods and Protocols offers both basic and clinical researchers proven cutting-edge methods for analyzing mtDNA and illuminating the role it plays in the aging process, apoptosis, and possibly some cancers.

Dalla quarta di copertina:

Mutations within mitochondrial DNA (mtDNA) and the nuclear genes involved in the maintenance of mitochondrial DNA have been linked to a wide range of human diseases, including several of the most common diseases of aging. In Mitochondrial DNA: Methods and Protocols internationally recognized authorities describe in great detail the methods they have perfected to analyze mtDNA and the proteins involved in its maintenance. The analytical techniques cover the purification of mtDNA from a variety of sources and the analysis of DNA for both deletions, point mutations, and damage, for replication intermediates, and for following the fate of mtDNA outside of the mitochondria. Additional analytical methods are presented for analyzing the proteins and enzymes that maintain mtDNA. Each readily reproducible protocol includes step-by-step instructions, tips on avoiding pitfalls and extending the method to other situation, and introductory material explaining the theory behind the process.
Comprehensive and timely, Mitochondrial DNA: Methods and Protocols offers both basic and clinical researchers proven cutting-edge methods for analyzing the role mtDNA plays in the aging process, apoptosis, and possibly some cancers, and for investigating the cause of mitochondrial dysfunction and disease.