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Hardcover. Condizione: gut. 2010 | 3rd ed. 2011. Fully revised and updated, Flow Cytometry Protocols highlights the expanding contribution of flow cytometry to basic biological research and diagnostic medicine. The text presents cutting-edge topics, such as polychromatic, quantitative, and high throughput flow cytometry, and more. With rapid improvements in instrumentation, lasers, fluorophores, and data analysis software, flow cytometry is riding the crest of unprecedented, innovative advances. This thoroughly revised and up-to-date third edition of Flow Cytometry Protocols highlights the expanding contribution of flow cytometry to basic biological research and diagnostic medicine. Written by leading experts in the field, the book presents cutting-edge topics such as polychromatic, quantitative, and high throughput flow cytometry, novel multiparametric data analysis which breaks the dimensionality barrier, standard practice and safety measures for aerosol-generating cell sorting, conventional and imaging flow cytometry as well as minimalist imaging cytometry. As a volume in the highly successful Methods in Molecular Biology series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and extensive notes on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Flow Cytometry Protocols, Third Edition presents established as well as new flow cytometric methodologies in order to introduce beginning users to basic applications while opening new avenues of innovation for seasoned users. Content: 1. Flow Cytometry: An Introduction Alice L. Givan 2. Breaking the Dimensionality Barrier C. Bruce Bagwell 3. Quantitative Fluorescence Measurements with Multicolor Flow Cytometry Lili Wang, Adolfas K. Gaigalas, and Ming Yan 4. Quantum Dots for Quantitative Flow Cytometry Yang Wu, Larry A. Sklar, and Tione Buranda 5. Bead-Based Multiplexed Analysis of Analytes by Flow Cytometry Henri C. van der Heyde and Irene Gramaglia 6. Flow-Based Combinatorial Antibody Profiling: An Integrated Approach to Cell Characterization Shane Bruckner, Ling Wang, Ruiling Yuan, Perry Haaland, and Amitabh Gaur 7. Tracking Immune Cell Proliferation and Cytotoxic Potential Using Flow Cytometry Joseph D. Tario, Jr., Katharine A. Muirhead, Dalin Pan, Mark E. Munson, and Paul K. Wallace 8. Multiparameter Intracellular Cytokine Staining Patricia Lovelace and Holden T. Maecker 9. Phospho Flow Cytometry Methods for Analysis of Kinase Signaling in Cell Lines and Primary Human Blood Samples Peter O. Krutzik, Angelica Trejo, Kenneth R. Schulz, and Garry P. Nolan 10. Multiparametric Analysis of Apoptosis by Flow Cytometry William G. Telford, Akira Komoriya, Beverly Z. Packard, and C. Bruce Bagwell 11. Multiparameter Cell Cycle Analysis James W. Jacobberger, R. Michael Sramkoski, and Tammy Stefan 12. Rare Event Detection and Analysis in Flow Cytometry: Bone Marrow Mesenchymal Stem Cells, Breast Cancer Stem/Progenitor Cells in Malignant Effusions, and Pericytes in Disaggregated Adipose Tissue Ludovic Zimmerlin, Vera S. Donnenberg, and Albert D. Donnenberg 13. Flow Cytometric-Based Identification of Immature Myeloerythroid Development Cornelis J.H. Pronk and David Bryder 14. Flow Cytometry Immunophenotyping of Hematolymphoid Neoplasia Katherine R. Calvo, Catharine S. McCoy, and Maryalice Stetler-Stevenson 15. Flow Cytometry Assays in Primary Immunodeficiency Diseases Maurice R.G. O'Gorman, Joshua Zollett, and Nicolas Bensen 16. Flow Cytometric Analysis of Microparticles Henri C. van der Heyde, Irene Gramaglia, Valery Combes, Thaddeus C. George, and Georges E. Grau 17. Noncytotoxic DsRed Derivatives for Whole-Cell Labeling Rita L. Strack, Robert J. Keenan, and Benjamin S. Glick 18. Flow Cytometric FRET Analysis of Protein Interaction Gyoergy Vereb, Peter Nagy, and Janos Szoellosi 19. Fluorescent Protein-Assisted Purification for Gene Expression Profiling M. Raza Zaidi, Chi-Ping Day, and Glenn Merlino 20. Multiparametric Analysis, Sorting, and Transcriptional Profiling of Plant Protoplasts and Nuclei According to Cell Type David W. Galbraith, Jaroslav Janda, and Georgina M. Lambert 21. Lentiviral Fluorescent Protein Expression Vectors for Biotinylation Proteomics Irene Riz, Teresa S. Hawley, and Robert G. Hawley 22. Standard Practice for Cell Sorting in a BSL-3 Facility Stephen P. Perfetto, David R. Ambrozak, Richard Nguyen, Mario Roederer, Richard A. Koup, and Kevin L. Holmes 23. The Cytometric Future: It Ain't Necessarily Flow! Howard M. Shapiro Reihe/Serie Methods in Molecular Biology ; 699 Zusatzinfo 26 Tables, black and white; XII, 486 p. Verlagsort Totowa, NJ Sprache englisch Maße 178 x 254 mm Studium 1. Studienabschnitt Vorklinik Histologie Embryologie Studium 2. Studienabschnitt Klinik Anamnese Körperliche Untersuchung Naturwissenschaften Biologie Genetik Molekularbiologie Technik Umwelttechnik Biotechnologie ISBN-10 1-61737-949-2 1617379492 ISBN-13 978-1-61737-949-9 9781617379499 Flow Cytometry Protocols Methods in Molecular Biology Band 699 [Hardcover] Hawley, Teresa S. Hawley, Robert In englischer Sprache. 485 pages. 178 x 254 mm.…

Soft cover. Condizione: Good. 18 books: Sex & [and] Love Among the Poor. Trainer, Russell. Ballantine Books, 1968. 314p. paperback, covers bumped/scuffed, binding tight, text clean + Sexual Surrender in Women. Morse, Benjamin. Monarch Books (1962) 155p. paperback, covers bumped/scuffed, binding tight, text clean/unmarked + Your Sexual Personality: A Guide to Sexual Adequacy and Happiness. Kappas, John G., Panorama, 1982, 189p, trade pb, covers bumped/scuffed, clean text, solid binding + The Sensual Male: How to "Make It' 365 Days a Year, Paul Warren, Pinnacle Books, 1971, 186p, pb, covers bumped/scuffed/creased, text clean, semi-solid binding + THE INTIMATE KISS. G. Legman. Paperback Library, New York, 1971. 286p. paperback, covers bumped/scuffed, binding tight, text clean/unmarked last page has two 1/2 edge tears + SEXERCISES : Isometric and Isotonic. Edward O'Relly. Pocket Books, 1968. paperback, covers bumped/scuffed, binding tight, text clean/unmarked, + The G Spot: And Other Discoveries About Human Sexuality. Ladas, Alice Kahn, Whipple, Beverly, Perry, John D. Dell. 1982. 236p. paperback, covers bumped/scuffed, binding tight, text clean/unmarked, name/address stamp front endpaper + Sex Can Be An Art! Cooke, Charles E. and Eleanore Ross. Signet. 1968. 192p. paperback, covers bumped/scuffed, binding tight, text clean/unmarked + Nice Girls Do and Now You Can Too. Irene Kassorla. Stratford Press. 1980. 237p. hardcover with dust jacket, dust jacket bumped/scuffed/price-clipped, boards lightly bumped/scuffed, binding tight, text clean/unmarked, NOT xlib--SIGNED BY AUTHOR + The Anatomy of a Love Affair: A Guide to Sex for the Girl Who Says "Yes!" Bourne, Evelyn. Pyramid. 1965. 125p. paperback, covers bumped/scuffed, binding tight, text clean creased front cover + How to Drive Your Man Wild in Bed. Graham Masterton. Signet. 1976. 223p. paperback, covers bumped/scuffed, binding tight, text clean + Passive Men, Wild Women. Pierre Mornell. Ballantine. 1979. 147p. paperback, covers bumped/scuffed, binding tight, text clean/unmarked + What Turns Women On. Robert Chartham. Penthouse/Ballantine. 1974. 207p. paperback, covers bumped/scuffed, binding tight, text clean/unmarked + The Sensuous Man: The First How-To Book for the Man Who Wants to be a Great Lover. M. Dell. 213p. paperback, covers bumped/scuffed, binding tight, text clean/unmarked + WHAT YOU ALWAYS WANTED TO KNOW ABOUT THE SEXUAL PROGRESSIVES (BUT WERE AFRAID TO ASK). EXCLUSIVE PHOTO-DOCUMENTATION EXPLAINED BY ANTHONY CROWELL, PH.D. Crowell, Anthony. Global Press/Eros Pub. 1972. 263p. paperback, covers bumped/scuffed, binding tight, text clean/unmarked + The Puritan Jungle, America's Sexual Underground, HARRIS, SARA, Pocket Book, N.Y., 1970, 256p, pb, covers bumped/scuffed/creased, text clean, solid binding + The Sexual Scene, Gagnon, John H., & Simon, William, Eds., Transaction, 1970, 150p, trade pb, covers bumped/scuffed/creased, clean text, solid binding--several essays, groovy look at sex in the 60s + An Analysis of Human Sexual Response. Ruth and Edward Brecher [Editors] Little Brown. 1966. 318p. hardcover with dust jacket, dust jacket lightly bumped/scuffed, boards clean/square, binding tight, text clean/unmarked, NOT xlib 85.00. EXTRA SHIPPING (NOT MUCH!).…