Humana dez 2008 (72 risultati)
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Buch. Condizione: Neu. Neuware - As the expense of treating a growing number of end-stage kidney disease patients increases, greater attention has been paid to prevention and early treatment. The study of renal disease, however, suffers due to the complex nature of renal anatomy and physiology and the plethora of different cell…types found in the kidney. In Kidney Research: Experimental Protocols, top experts in the field seek to aid researchers by providing a number of specialized techniques developed to examine this intricate system. Through both well-established and novel methods, this volume explores the preparation and culture of the main cell types used to study renal disease mechanisms, the common animal models used to mimic the various forms of human renal disease, and specific applications and techniques used in vivo and in vitro. Written in the highly successful Methods in Molecular Biology(TM) series format, the chapters contain step-by-step, readily reproducible laboratory protocols, lists of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.Essential and cutting-edge, Kidney Research: Experimental Protocols delivers invaluable guidance to new and experienced laboratory researchers delving into the sophisticated study of the kidney.
Lingua: Inglese
Editore: Springer, Humana Dez 2008, 2008
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Da: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, GermaniaBuchWeltWeit Ludwig Meier e.K.
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Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This is a book about prime numbers, congruences, secret messages, and elliptic curves that you can read cover to cover. It grew out of undergr- uate courses that the author taught at Harvard, UC San Diego, and the University of Washington…. The systematic study of number theory was initiated around 300B. C. when Euclid proved that there are in nitely many prime numbers, and also cleverly deduced the fundamental theorem of arithmetic, which asserts that every positive integer factors uniquely as a product of primes. Over a thousand years later (around 972A. D. ) Arab mathematicians formulated the congruent number problem that asks for a way to decide whether or not a given positive integer n is the area of a right triangle, all three of whose sides are rational numbers. Then another thousand years later (in 1976), Di e and Hellman introduced the rst ever public-key cryptosystem, which enabled two people to communicate secretely over a public communications channel with no predeterminedsecret; this invention and the ones that followed it revolutionized the world of digital communication. In the 1980s and 1990s, elliptic curves revolutionized number theory, providing striking new insights into the congruent number problem, primality testing, publ- key cryptography, attacks on public-key systems, and playing a central role in Andrew Wiles' resolution of Fermat's Last Theorem. 180 pp. Englisch.
Lingua: Inglese
Editore: Springer, Humana Dez 2008, 2008
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Da: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, GermaniaBuchWeltWeit Ludwig Meier e.K.
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Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Several years ago I was invited to an American university to give one-term graduate course on Siegel modular forms, Hecke operators, and related zeta functions. The idea to present in a concise but basically complete and self-conta…ined form an int- duction to an important and developing area based partly on my own work attracted me. I accepted the invitation and started to prepare the course. Unfortunately, the visit was not realized. But the idea of such a course continued to be alive till after a number of years this book was nally completed. I hope that this short book will serve to attract young researchers to this beautiful eld, and that it will simplify and make more pleasant the initial steps. No special knowledge is presupposed for reading this book beyond standard courses in algebra and calculus (one and several variables), although some skill in working with mathematical texts would be helpful. The reader will judge whether the result was worth the effort. Dedications. The ideas of Goro Shimura exerted a deep in uence on the number theory of the second half of the twentieth century in general and on the author's formation in particular. When Andre ` Weil was signing a copy of his 'Basic Number Theory' to my son, he wrote in Russian, 'To Fedor Anatolievich hoping that he will become a number theoretist'. Fedor has chosen computer science. Now I pass on the idea to Fedor's daughter, Alexandra Fedorovna. 196 pp. Englisch.
Lingua: Inglese
Editore: Springer, Humana Dez 2008, 2008
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Da: buchversandmimpf2000, Emtmannsberg, BAYE, Germaniabuchversandmimpf2000
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Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This is a textbook about classical elementary number theory and elliptic curves. The first part discusses elementary topics such as primes, factorization, continued fractions, and quadratic forms, in the context of cryptography, computation,…and deep open research problems. The second part is about elliptic curves, their applications to algorithmic problems, and their connections with problems in number theory. The intended audience of this book is an undergraduate with some familiarity with basic abstract algebra.Springer-Verlag KG, Sachsenplatz 4-6, 1201 Wien 180 pp. Englisch.
Lingua: Inglese
Editore: Humana Press Dez 2008, 2008
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Da: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, GermaniaBuchWeltWeit Ludwig Meier e.K.
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Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Biosensors combine biological recognition elements and signal conversion elements into a biodetection system. They have been developed for a wide variety of biodetection applications, offering the advantages of increased speed and ease of… use compared to traditional detection methods. In Biosensors and Biodetection: Methods and Protocols, leading experts describe the major technologies in the field in extensive technical detail, allowing readers both to understand the technology and to construct similar devices. Volume 1: Optical-Based Detectors delves into direct and indirect optical detectors, including methods involving surface plasmon resonance, interferometric sensors, CCD based detectors, and spectrometers, among many other cutting-edge technologies. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include brief introductions to the subjects, lists of the necessary materials, step-by-step, readily reproducible protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.Comprehensive and up-to-date, Biosensors and Biodetection: Methods and Protocols is an ideal, user-friendly guide to this vital, versatile technology and a perfect tool for those who wish to further the field. 472 pp. Englisch.
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Da: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, GermaniaBuchWeltWeit Ludwig Meier e.K.
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Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Interstitial lung disease (ILD) is a broad category of lung diseases that includes more than 150 disorders characterized by scarring or fibrosis of the lungs. In Pulmonary Arterial Hypertension and Interstitial Lung Disease: A Clinical Gu…ide, renowned experts provide a state-of-the-art overview of the problems seen by physicians in the clinical management of ILDs. Divided into two sections, the first part provides and update on general issues and introduces both interstitial lung disease and associated pulmonary hypertension. A detailed analysis of the pathology of the various interstitial lung diseases is also provided. The second part addresses specific categories of disease. Bronchiolitis, hypersensitivity pneumonitis, and other conditions are covered, and the use of inspiratory and expiratory high resolution CT scan is discussed as well. This important new text is an invaluable resource for the practicing physician who must be aware of the broad and troubling manifestations of interstitial lung disease. 280 pp. Englisch.
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Da: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, GermaniaBuchWeltWeit Ludwig Meier e.K.
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Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -During recent years, research has greatly expanded our understanding of the sophisticated molecular network of responses which enable plants to develop, survive and propagate under a wide range of conditions. In Plant Signal Transduction:… Methods and Protocols, an international panel of experts provide well-established methods vital to analyzing plant signal transduction on the molecular level. Featuring experimental procedures on several of the most popular model organisms, the volume focuses on in planta analyses and the proteins involved in signal transduction in order to aid with the establishment of laboratory techniques or the modification of the protocols for other plants. As part of the highly successful Methods in Molecular Biology series, the chapters include brief introductions to the subject, lists of necessary materials, readily reproducible laboratory protocols, and tips on trouble-shooting and avoiding known pitfalls. Comprehensive and cutting-edge, Plant Signal Transduction: Methods and Protocols will benefit plant scientists wishing to improve their experimental approaches and delve further into this exciting and important field of study 380 pp. Englisch.
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Da: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, GermaniaBuchWeltWeit Ludwig Meier e.K.
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Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Gene Transfer into Hematopoietic Cells: From Basic Science to Clinical Application Christopher Baum 1. The Potential of Gene Transfer into Hematopoietic Vectors with the potential for stable transgene integration are Cells widely used in…basic hematology and clinical trials of gene me- cine. In basic research, both gain-of-function and loss-of-fu- tion situations of individual genes can be created by gene transfer, leading to a wide range of applications in developmental biology, stem cell biology, immunology, leukemia research, and human genetics. With the first evidence of successful modification of murine hematopoietic cells using retroviral gene vectors (1, 2), researchers have also explored the therapeutic potential of this approach. To date, the emerging discipline of gene therapy is a highly diversified field that offers entirely novel approaches to treat a great variety of human diseases (3). All hematopoietic cell types are of major interest in this context, since the modification of the hematopoietic stem cell population may potentially give rise to a completely transgenic hematopoiesis with the potential to cure genetic disorders or fight severe chronic infections, and the targeting of mature cells such as lymphocytes or antigen-p- senting dendritic cells offers all types of transient and semiper- nent modifications of the immune system. The unifying principle of gene medicine is the need to transfer complex nucleic acids cells that do not contribute to the germline (somatic cells). 528 pp. Englisch.
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Da: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, GermaniaBuchWeltWeit Ludwig Meier e.K.
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Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Acting principally to control patterns of gene expression, nuclear receptors play vital roles during embryonic development and in the regulation of metabolic and reproductive functions in adult life, which proves this superfamily of ligan…d-activated transcription factors to be a crucial part of biological life. In The Nuclear Receptor Superfamily: Methods and Protocols, expert researchers describe a range of molecular, structural and cell biological techniques currently used to investigate the structure-function of nuclear receptors, together with experimental approaches that may lead to new therapeutic strategies for treating nuclear receptor-associated diseases. Written in the highly successful Methods in Molecular Biology(TM) series format, the chapters in this volume contain brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as notes from the experts to highlight tips on troubleshooting and avoiding known pitfalls.Cutting-edge and easy to use, The Nuclear Receptor Superfamily: Methods and Protocols provides beneficial and time-saving guidance for all those undertaking research in this ever-growing field of study. 296 pp. Englisch.
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Da: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, GermaniaBuchWeltWeit Ludwig Meier e.K.
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Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -In the 21st century, academic medical centers across the United States continue to make scientific breakthroughs, to make improvements in patient care, and to p- vide the most advanced information and guidance in matters affecting public…health. The signs of growth are everywhere-in new research buildings, new pa- nerships with industry, new forms of molecular medicine, and new sensitivity to the role of the human spirit in healing. This growth is due in large part to the dedication and productivity of our faculty, who are providing more patient care, more research, more teaching, and more community service than ever before. Today, there are roughly 135,000 physicians, scientists, and other faculty wo- ing at approximately 125 academic medical centers around the country. Increasingly, they are asked to do more with less. Since the 1990s, academic medical centers in the United States have lost the financial margin they once enjoyed, thereby putting new pressures on research, education, and clinical care. Medical school faculty, previously given funded time for teaching and research, are increasingly drafted to bring in clinical revenues to cover their salaries. Dedicated to the missions of research, teaching, and care, our faculty have responded well to these challenges and perform at a very high level. However, we are beginning to see the results of ongoing stress. 264 pp. Englisch.
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Da: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, GermaniaBuchWeltWeit Ludwig Meier e.K.
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Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book is a collection of protocols, to provide novel techniques for the study of the biology of T lymphocytes. The methods described in this book do not cover all of the techniques currently used to study T cell-mediated immune respon…ses for the simple reason that T cell immunology is probably the immunological discipline which can be investigated with the widest variety of approaches. Thechoiceofchapterswasmadetakingintoaccounttwopoints:First,manyofthe techniquesthathavebeen usedfor sometimehave beenupgraded duringthe pastfew years given the greater availability of a variety of products (i.e. cytokines, chemokines, monoclonal antibodies), of refined technical devices (i.e. novel cell culture and cell analysis equipments), and the development of novel instrumentation (i.e. multipa- metric flow cytometers, confocal microscopes). Therefore, in several chapters ''old techniques'', which remain fundamental to T cell immunology, are described in their ''modern'' versions. Secondly, the technical advancement has generated the possibility to establish novel assays to investigate T cell physiology. This is reflected in the chapters which describe the protocols that allow use of these modern approaches. The preparation of this book has required participation of several scientists, all leading experts in their respective fields. Without their enthusiastic participation, this work would not have been possible. Therefore, I thank all authors for their contri- tionsandacceptallcriticismformissingparts,orinformationordetails,forwhichonly I am responsible. I hope these protocols will be useful for young investigators who approach for the first time the complex field of immunology and for those more experienced scientists who look for concise and efficacious descriptions of novel methods. 184 pp. Englisch.
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Da: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, GermaniaBuchWeltWeit Ludwig Meier e.K.
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Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -When I was rst approached by Dr. Hengli Tang about writing the foreword to this book, the second edition of Hepatitis C Virus, I was a little puzzled: Book forewords are penned by persons far more famous than I am. Surely there were bette…r people to write this, with a handful of the true visionaries in the eld immediately coming to my mind. However, after some thought and self-re ection, I realized that I might just be the right person for the job. I remember the publication of the rst edition of Hepatitis C Virus in 1998, the year I began reading about hepatitis C virus with great interest. I purchased a copy of the rst edition from my meager graduate student stipend and read it from cover to cover, along with a variety of hepatitis C publications, to try to get a feel if this hepatitis C virus eld was something I might want to work on in the future. At the time, the rst edition was among the most comprehensive collection of hepatitis C protocols and methods available in one volume. I was amazed at the progress that had been made in hepatitis C research, and even more amazed as I learned more about the experimental challenges facing the eld at that time. 484 pp. Englisch.
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Da: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, GermaniaBuchWeltWeit Ludwig Meier e.K.
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Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Biosensors combine biological recognition elements and signal conversion elements into a biodetection system. They have been developed for a wide variety of biodetection applications, offering the advantages of increased speed and ease of… use compared to traditional detection methods. In Biosensors and Biodetection: Methods and Protocols, leading experts describe the major technologies in the fieldin extensivetechnical detail, allowing readers both to understand the technology and toconstruct similar devices.Volume 2: Electrochemical and Mechanical Detectors, Lateral Flow and Ligands for Biosensorsfocuses on direct measurement sensors, indirect methods, ligands, and related technologies, including methods involving electrochemical detectors, recognition ligands, antibodies, aptamers, and peptides, amongst many other subjects.Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include brief introductions to the topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.Comprehensive and up-to-date, Biosensors and Biodetection: Methods and Protocols is an ideal, user-friendly guide to this vital, versatile technology and a perfect tool for those who wish to further the field. 484 pp. Englisch.
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Da: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, GermaniaBuchWeltWeit Ludwig Meier e.K.
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Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Over the past few years, DNA methylation technologies and our knowledge of DNA methylation patterns have been advancing at a breathtaking pace. Due to this fact, DNA Methylation: Methods and Protocols, Second Edition completely revises, u…pdates, and expands upon the popular first edition with the most current novel techniques, easier to use and more refined by the tested experience of leading experts. This revision reflects contemporary study of the subject: the analysis of gene-specific DNA methylation patterns has been complemented by genome-wide approaches, and epigenomics takes a central place. Written in the highly successful Methods in Molecular Biology(TM) series format, the chapters in this volume present brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, DNA Methylation: Methods and Protocols, Second Edition is the perfect resource for scientists who wish to further our understanding of the molecular processes that determine the genomic DNA methylation landscape and for those who aim to identify and implement DNA methylation-based biomarkers for clinical and diagnostic applications. 420 pp. Englisch.
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Da: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, GermaniaBuchWeltWeit Ludwig Meier e.K.
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Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -As experimentation and clinical trials with first generation anti-angiogenic agents have yielded results and our understanding of the biology and physiology of blood and lymphatic vessels has increased, a new angiogenesis volume swiftly b…ecame a necessity. Angiogenesis Protocols, Second Edition remains true to its original vision of providing a single source for angiogenesis researchers, irrespective of levels of resource and expertise, by collecting a range of methods for cell isolation and assessing angiogenesis in vivo or in vitro. This information, however, is expanded to include chapters on circulating endothelial progenitor cells, angiogenic signalling pathways, imaging of angiogenesis, and measurement of tissue blood flow. Written in the Methods in Molecular Biology(TM) series format, the chapters provide step-by-step laboratory protocols, lists of necessary materials and reagents, and a Notes section, which details tips on troubleshooting and avoiding known pitfalls.Extensive and cutting-edge, Angiogenesis Protocols, Second Edition is not only a practical handbook for key techniques, but also an informative and enjoyable read for all those interested, no matter how directly, in angiogenesis. 380 pp. Englisch.
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Da: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, GermaniaBuchWeltWeit Ludwig Meier e.K.
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Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -In this new age of science and technology, microchip methods of analysis have developed into an essential tool for biomedical researchers. Microchip Methods in Diagnostics looks at this increasingly miniaturized technology, focusing on DN…A microarrays, protein microarrays, and labs-on-chips, and supporting the introduction of new diagnostic methods based on microtechnologies. The work provides a background on the technological aspects of each of these methods, followed by chapters describing the methods with regard to specific applications. Since it uses only those processes which have reached a certain level of maturation, this volume is able to provide approaches that are applicable in practice even by the non-expert. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.An innovative and cutting-edge work, Microchip Methods in Diagnostics strives to bring the technology of microchips even further into the world of diagnostics, predicting incredible benefits for patients in the years to come. 196 pp. Englisch.
Lingua: Inglese
Editore: Springer, Humana Dez 2008, 2008
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Da: buchversandmimpf2000, Emtmannsberg, BAYE, Germaniabuchversandmimpf2000
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Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Several years ago I was invited to an American university to give one-term graduate course on Siegel modular forms, Hecke operators, and related zeta functions. The idea to present in a concise but basically complete and self-contained… form an int- duction to an important and developing area based partly on my own work attracted me. I accepted the invitation and started to prepare the course. Unfortunately, the visit was not realized. But the idea of such a course continued to be alive till after a number of years this book was nally completed. I hope that this short book will serve to attract young researchers to this beautiful eld, and that it will simplify and make more pleasant the initial steps. No special knowledge is presupposed for reading this book beyond standard courses in algebra and calculus (one and several variables), although some skill in working with mathematical texts would be helpful. The reader will judge whether the result was worth the effort. Dedications. The ideas of Goro Shimura exerted a deep in uence on the number theory of the second half of the twentieth century in general and on the author¿s formation in particular. When Andre ` Weil was signing a copy of his ¿Basic Number Theory¿ to my son, he wrote in Russian, ¿To Fedor Anatolievich hoping that he will become a number theoretist¿. Fedor has chosen computer science. Now I pass on the idea to Fedor¿s daughter, Alexandra Fedorovna.Springer-Verlag KG, Sachsenplatz 4-6, 1201 Wien 196 pp. Englisch.
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Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Gene Transfer into Hematopoietic Cells: From Basic Science to Clinical Application Christopher Baum 1. The Potential of Gene Transfer into Hematopoietic Vectors with the potential for stable transgene integration are Cells widely used in basi…c hematology and clinical trials of gene me- cine. In basic research, both gain-of-function and loss-of-fu- tion situations of individual genes can be created by gene transfer, leading to a wide range of applications in developmental biology, stem cell biology, immunology, leukemia research, and human genetics. With the first evidence of successful modification of murine hematopoietic cells using retroviral gene vectors (1, 2), researchers have also explored the therapeutic potential of this approach. To date, the emerging discipline of gene therapy is a highly diversified field that offers entirely novel approaches to treat a great variety of human diseases (3). All hematopoietic cell types are of major interest in this context, since the modification of the hematopoietic stem cell population may potentially give rise to a completely transgenic hematopoiesis with the potential to cure genetic disorders or fight severe chronic infections, and the targeting of mature cells such as lymphocytes or antigen-p- senting dendritic cells offers all types of transient and semiper- nent modifications of the immune system. The unifying principle of gene medicine is the need to transfer complex nucleic acids cells that do not contribute to the germline (somatic cells).Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 528 pp. Englisch.
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Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -As experimentation and clinical trials with first generation anti-angiogenic agents have yielded results and our understanding of the biology and physiology of blood and lymphatic vessels has increased, a new angiogenesis volume swiftly becam…e a necessity. Angiogenesis Protocols, Second Edition remains true to its original vision of providing a single source for angiogenesis researchers, irrespective of levels of resource and expertise, by collecting a range of methods for cell isolation and assessing angiogenesis in vivo or in vitro. This information, however, is expanded to include chapters on circulating endothelial progenitor cells, angiogenic signalling pathways, imaging of angiogenesis, and measurement of tissue blood flow. Written in the Methods in Molecular Biology¿ series format, the chapters provide step-by-step laboratory protocols, lists of necessary materials and reagents, and a Notes section, which details tips on troubleshooting and avoiding known pitfalls.Extensive and cutting-edge, Angiogenesis Protocols, Second Edition is not only a practical handbook for key techniques, but also an informative and enjoyable read for all those interested, no matter how directly, in angiogenesis.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 380 pp. Englisch.
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Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Interstitial lung disease (ILD) is a broad category of lung diseases that includes more than 150 disorders characterized by scarring or fibrosis of the lungs. In Pulmonary Arterial Hypertension and Interstitial Lung Disease: A Clinical Guide,… renowned experts provide a state-of-the-art overview of the problems seen by physicians in the clinical management of ILDs. Divided into two sections, the first part provides and update on general issues and introduces both interstitial lung disease and associated pulmonary hypertension. A detailed analysis of the pathology of the various interstitial lung diseases is also provided. The second part addresses specific categories of disease. Bronchiolitis, hypersensitivity pneumonitis, and other conditions are covered, and the use of inspiratory and expiratory high resolution CT scan is discussed as well. This important new text is an invaluable resource for the practicing physician who must be aware of the broad and troubling manifestations of interstitial lung disease.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 280 pp. Englisch.
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Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Over the past few years, DNA methylation technologies and our knowledge of DNA methylation patterns have been advancing at a breathtaking pace. Due to this fact, DNA Methylation: Methods and Protocols, Second Edition completely revises, updat…es, and expands upon the popular first edition with the most current novel techniques, easier to use and more refined by the tested experience of leading experts. This revision reflects contemporary study of the subject: the analysis of gene-specific DNA methylation patterns has been complemented by genome-wide approaches, and epigenomics takes a central place. Written in the highly successful Methods in Molecular Biology¿ series format, the chapters in this volume present brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, DNA Methylation: Methods and Protocols, Second Edition is the perfect resource for scientists who wish to further our understanding of the molecular processes that determine the genomic DNA methylation landscape and for those who aim to identify and implement DNA methylation-based biomarkers for clinical and diagnostic applications.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 420 pp. Englisch.
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Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -During recent years, research has greatly expanded our understanding of the sophisticated molecular network of responses which enable plants to develop, survive and propagate under a wide range of conditions. In Plant Signal Transduction: Met…hods and Protocols, an international panel of experts provide well-established methods vital to analyzing plant signal transduction on the molecular level. Featuring experimental procedures on several of the most popular model organisms, the volume focuses on in planta analyses and the proteins involved in signal transduction in order to aid with the establishment of laboratory techniques or the modification of the protocols for other plants. As part of the highly successful Methods in Molecular Biology¿ series, the chapters include brief introductions to the subject, lists of necessary materials, readily reproducible laboratory protocols, and tips on trouble-shooting and avoiding known pitfalls.Comprehensive and cutting-edge, Plant Signal Transduction: Methods and Protocols will benefit plant scientists wishing to improve their experimental approaches and delve further into this exciting and important field of studySpringer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 380 pp. Englisch.
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Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This book is a collection of protocols, to provide novel techniques for the study of the biology of T lymphocytes. The methods described in this book do not cover all of the techniques currently used to study T cell-mediated immune responses…for the simple reason that T cell immunology is probably the immunological discipline which can be investigated with the widest variety of approaches. Thechoiceofchapterswasmadetakingintoaccounttwopoints:First,manyofthe techniquesthathavebeen usedfor sometimehave beenupgraded duringthe pastfew years given the greater availability of a variety of products (i.e. cytokines, chemokines, monoclonal antibodies), of refined technical devices (i.e. novel cell culture and cell analysis equipments), and the development of novel instrumentation (i.e. multipa- metric flow cytometers, confocal microscopes). Therefore, in several chapters ¿¿old techniques¿¿, which remain fundamental to T cell immunology, are described in their ¿¿modern¿¿ versions. Secondly, the technical advancement has generated the possibility to establish novel assays to investigate T cell physiology. This is reflected in the chapters which describe the protocols that allow use of these modern approaches. The preparation of this book has required participation of several scientists, all leading experts in their respective fields. Without their enthusiastic participation, this work would not have been possible. Therefore, I thank all authors for their contri- tionsandacceptallcriticismformissingparts,orinformationordetails,forwhichonly I am responsible. I hope these protocols will be useful for young investigators who approach for the first time the complex field of immunology and for those more experienced scientists who look for concise and efficaciousdescriptions of novel methods.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 184 pp. Englisch.
Lingua: Inglese
Editore: Humana Dez 2008, 2008
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Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Biosensors combine biological recognition elements and signal conversion elements into a biodetection system. They have been developed for a wide variety of biodetection applications, offering the advantages of increased speed and ease of use… compared to traditional detection methods. In Biosensors and Biodetection: Methods and Protocols, leading experts describe the major technologies in the field in extensive technical detail, allowing readers both to understand the technology and to construct similar devices. Volume 1: Optical-Based Detectors delves into direct and indirect optical detectors, including methods involving surface plasmon resonance, interferometric sensors, CCD based detectors, and spectrometers, among many other cutting-edge technologies. Written in the highly successful Methods in Molecular Biology¿ series format, chapters include brief introductions to the subjects, lists of the necessary materials, step-by-step, readily reproducible protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.Comprehensive and up-to-date, Biosensors and Biodetection: Methods and Protocols is an ideal, user-friendly guide to this vital, versatile technology and a perfect tool for those who wish to further the field.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 472 pp. Englisch.
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Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Biosensors combine biological recognition elements and signal conversion elements into a biodetection system. They have been developed for a wide variety of biodetection applications, offering the advantages of increased speed and ease of use… compared to traditional detection methods. In Biosensors and Biodetection: Methods and Protocols, leading experts describe the major technologies in the field in extensive technical detail, allowing readers both to understand the technology and to construct similar devices. Volume 2: Electrochemical and Mechanical Detectors, Lateral Flow and Ligands for Biosensors focuses on direct measurement sensors, indirect methods, ligands, and related technologies, including methods involving electrochemical detectors, recognition ligands, antibodies, aptamers, and peptides, amongst many other subjects. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include brief introductions to the topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.Comprehensive and up-to-date, Biosensors and Biodetection: Methods and Protocols is an ideal, user-friendly guide to this vital, versatile technology and a perfect tool for those who wish to further the field.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 484 pp. Englisch.
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Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Genetic Programming Theory and Practice VI was developed from the sixth workshop at the University of Michigan¿s Center for the Study of Complex Systems to facilitate the exchange of ideas and information related to the rapidly advancing fiel…d of Genetic Programming (GP). Contributions from the foremost international researchers and practitioners in the GP arena examine the similarities and differences between theoretical and empirical results on real-world problems. The text explores the synergy between theory and practice, producing a comprehensive view of the state of the art in GP application.These contributions address several significant interdependent themes which emerged from this year¿s workshop, including: (1) Making efficient and effective use of test data. (2) Sustaining the long-term evolvability of our GP systems. (3) Exploiting discovered subsolutions for reuse. (4) Increasing the role of a Domain Expert.Springer-Verlag KG, Sachsenplatz 4-6, 1201 Wien 288 pp. Englisch.
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Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Acting principally to control patterns of gene expression, nuclear receptors play vital roles during embryonic development and in the regulation of metabolic and reproductive functions in adult life, which proves this superfamily of ligand-ac…tivated transcription factors to be a crucial part of biological life. In The Nuclear Receptor Superfamily: Methods and Protocols, expert researchers describe a range of molecular, structural and cell biological techniques currently used to investigate the structure-function of nuclear receptors, together with experimental approaches that may lead to new therapeutic strategies for treating nuclear receptor-associated diseases. Written in the highly successful Methods in Molecular Biology¿ series format, the chapters in this volume contain brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as notes from the experts to highlight tips on troubleshooting and avoiding known pitfalls.Cutting-edge and easy to use, The Nuclear Receptor Superfamily: Methods and Protocols provides beneficial and time-saving guidance for all those undertaking research in this ever-growing field of study.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 296 pp. Englisch.
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Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -In the 21st century, academic medical centers across the United States continue to make scientific breakthroughs, to make improvements in patient care, and to p- vide the most advanced information and guidance in matters affecting public heal…th. The signs of growth are everywhere¿in new research buildings, new pa- nerships with industry, new forms of molecular medicine, and new sensitivity to the role of the human spirit in healing. This growth is due in large part to the dedication and productivity of our faculty, who are providing more patient care, more research, more teaching, and more community service than ever before. Today, there are roughly 135,000 physicians, scientists, and other faculty wo- ing at approximately 125 academic medical centers around the country. Increasingly, they are asked to do more with less. Since the 1990s, academic medical centers in the United States have lost the financial margin they once enjoyed, thereby putting new pressures on research, education, and clinical care. Medical school faculty, previously given funded time for teaching and research, are increasingly drafted to bring in clinical revenues to cover their salaries. Dedicated to the missions of research, teaching, and care, our faculty have responded well to these challenges and perform at a very high level. However, we are beginning to see the results of ongoing stress.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 264 pp. Englisch.
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Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -In this new age of science and technology, microchip methods of analysis have developed into an essential tool for biomedical researchers. Microchip Methods in Diagnostics looks at this increasingly miniaturized technology, focusing on DNA mi…croarrays, protein microarrays, and labs-on-chips, and supporting the introduction of new diagnostic methods based on microtechnologies. The work provides a background on the technological aspects of each of these methods, followed by chapters describing the methods with regard to specific applications. Since it uses only those processes which have reached a certain level of maturation, this volume is able to provide approaches that are applicable in practice even by the non-expert. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.An innovative and cutting-edge work, Microchip Methods in Diagnostics strives to bring the technology of microchips even further into the world of diagnostics, predicting incredible benefits for patients in the years to come.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 196 pp. Englisch.
Lingua: Inglese
Editore: Springer New York, Humana Dez 2008, 2008
Serie: Libro 46 di 544 - Advances in Experimental Medicine and Biology
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Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Calcium phosphate coatings ( 50 mm thick), especially those made with hyd- xyapatite (HA), produced by the plasma-spraying process have been succe- fully used on orthopedic and dental implants to improve fixation of these implants in bone. Th…in calcium phosphate coatings ( 10mm thick) formed by various techniques other than plasma spraying may be the successor of the current thick plasma-sprayed coatings because of their improved properties. Like plasma-sprayed HA coatings, these alternative calcium phosphate thin coatings are capable of enabling bone formation on their surfaces and forming a bond with the newly formed bone. In addition, the thin calcium phosphate coatings have shown better adhesion to substrates and are more stable in the biological environment because they have more uniform structure and com- sition than plasma-sprayed HA coatings. Moreover, some of these thin calcium phosphate coatings can be formed on all kinds of substrates including polymers and on the entire surfaces of complex geometries such as porous surfaces.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 356 pp. Englisch.
