Humana mai 2017 (33 risultati)

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Cell migration is a key component of many biological processes including embryonic development, immune responses, wound healing, organ regeneration, and cancer cell metastasis, thus making it an exciting and crucial field of study. The aim of Cell Migration: Developmental Methods and Protocols, Second Edition is to bring together a wide range of these techniques from the more basic migration assays, which are still the foundation of many cell migration studies, to state-of-the-art techniques and recent technical advances. Divided into three convenient parts, the volume begins with a number of basic in vitro migration assays including measurements of wound healing, cell scattering, invasion, and chemotaxis, as well as more complex measurements of transendothelial migration, the use of microfluidic chambers, and imaging cell migration in 3D. It continues with procedures for the imaging and measurement of cell migration in vivo including protocols for the use of chick, drosophila, and zebrafish embryos, and methods to measure metastatic spread and angiogenesis in mice, then concludes with a vital section on emerging techniques in the cell migration field including the use of TIRF, FRAP, and FRET microscopy. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes from the experts on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Cell Migration: Developmental Methods and Protocols, Second Edition provides a comprehensive catalogue of techniques for the study of cell migration that can be used as a useful reference source for any researcher who wishes to explore this significant area of cell biology. 476 pp. Englisch.…

Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -In DSP Architecture Design Essentials, authors Dejan Markovi¿ and Robert W. Brodersen cover a key subject for the successful realization of DSP algorithms for communications, multimedia, and healthcare applications. The book addresses the need for DSP architecture design that maps advanced DSP algorithms to hardware in the most power- and area-efficient way.The key feature of this text is a design methodology based on a high-level design model that leads to hardware implementation with minimum power and area. The methodology includes algorithm-level considerations such as automated word-length reduction and intrinsic data properties that can be leveraged to reduce hardware complexity. From a high-level data-flow graph model, an architecture exploration methodology based on linear programming is used to create an array of architectural solutions tailored to the underlying hardware technology.The book is supplemented with online material: bibliography, design examples, CAD tutorials and custom software.Springer-Verlag KG, Sachsenplatz 4-6, 1201 Wien 368 pp. Englisch.…

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Divided into two convenient sections, Protein Kinase Technologies collects contributions from experts in the field examining recent methodologies and techniques generally applicable to protein kinase research as well as to individual protein kinases which require special attention in neuroscience. These chapters will not only be practical instructions useful for readers' daily work in setting up and performing research but also thought-provoking and enjoyable reviews of recent advancements of individual protein kinases in neuroscience. Written for the highly successful Neuromethods series, this work contains the kind of detailed description and implementation advice that is crucial for getting optimal results. Authoritative and accessible, Protein Kinase Technologies seeks to foster cross-talk among investigators who study different protein kinases, and will also be beneficial for the entry of new investigators into this pivotal field. 380 pp. Englisch.…

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The significant biological subject, the permeability barrier, is incredibly diverse and vital for a vast assortment of crucial functions in the body. In Permeability Barrier: Methods and Protocols, a variety of experienced researchers contribute techniques to study this complex system in its many forms. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include brief introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Permeability Barrier: Methods and Protocols serves as an ideal guide for all scientists seeking to further our understanding of this vital area of research. 468 pp. Englisch.…

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Advances in methodologies and experimental models are pivotal to furthering our understanding of central nervous system (CNS) functions in mammals. Isolated Central Nervous System Circuits examines the application a variety of technologies to mostly active in vitro preparations from basically different CNS regions with a diversity of functions. From patch-clamp, multiphoton imaging, live fluorescent protein expression, or immunohistochemistry techniques to acute or 'organotypic' slices and en bloc preparations, this detailed volume features key protocols and examples for exciting results from the labs of renowned experts. Written for the popular Neuromethods series, chapters include the kind of thorough implementation advice that is vital for achieving successful results. Comprehensive and cutting-edge, Isolated Central Nervous System Circuits will greatly aid scientists to further improve models and to develop corresponding models of not yet intensively studied structures such as nucleus ruber, superior colliculus, or basal ganglia. 484 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This comprehensive reference covers the impact of dietary phosphorus in phosphorus physiology, public health and the pathogenesis of disease. Divided into three parts, the first section is an overview of the history of phosphorus and the regulation of phosphorus homeostasis. The second section focuses on specific matters related to phosphorus in the food supply. Clinical applications of the material presented in the preceding sections are pulled together in the third section - including the importance of both phosphorus excess and phosphorus deficiency for the pathogenesis of a wide variety of disease including kidney, cardiovascular, bone and oncologic diseases.Clinical Aspects of Natural and Added Phosphorus in Foods is an indispensable resource for understanding the growing importance of dietary phosphorus content in health and disease, enhancing patient care and moving forward the field of phosphorus science.Springer-Verlag KG, Sachsenplatz 4-6, 1201 Wien 288 pp. Englisch.…

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Mesenchymal Stem Cells have seen an unprecedented level of interest in the last decade, primarily due to their relative ease of isolation, the large numbers of cells present in the adult, and the ability to propagate these cells in culture. In Mesenchymal Stem Cell Assays and Applications, expert researchers from across the globe explore the latest techniques to propagate, characterize, and engineer this special cell type. Chapters outline a set of protocols and assays used by leading investigators in the field, providing standards that can be applied by all researchers to the population of cells used in their experiments. Composed in the highly successful Methods in Molecular Biology(TM) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.Ground-breaking and current, Mesenchymal Stem Cell Assays and Applications is a necessary handbook for all researchers working with this ambiguous population of cells. 552 pp. Englisch.…

Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Cell migration is a key component of many biological processes including embryonic development, immune responses, wound healing, organ regeneration, and cancer cell metastasis, thus making it an exciting and crucial field of study. The aim of Cell Migration: Developmental Methods and Protocols, Second Edition is to bring together a wide range of these techniques from the more basic migration assays, which are still the foundation of many cell migration studies, to state-of-the-art techniques and recent technical advances. Divided into three convenient parts, the volume begins with a number of basic in vitro migration assays including measurements of wound healing, cell scattering, invasion, and chemotaxis, as well as more complex measurements of transendothelial migration, the use of microfluidic chambers, and imaging cell migration in 3D. It continues with procedures for the imaging and measurement of cell migration in vivo including protocols for the use of chick, drosophila, and zebrafish embryos, and methods to measure metastatic spread and angiogenesis in mice, then concludes with a vital section on emerging techniques in the cell migration field including the use of TIRF, FRAP, and FRET microscopy. Written in the highly successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes from the experts on troubleshooting and avoiding known pitfalls.Comprehensive and up-to-date, Cell Migration: Developmental Methods and Protocols, Second Edition provides a comprehensive catalogue of techniques for the study of cell migration that can be used as a useful reference source for anyresearcher who wishes to explore this significant area of cell biology.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 476 pp. Englisch.…

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Over the last ten years, Green Fluorescent Proteins, along with the other spectral variants, have emerged from near obscurity to become a powerful and versatile tool in scientific research. In Viral Applications of Green Fluorescent Protein: Methods and Protocols, leading investigators from around the world contribute detailed examples of both the construction and application of fluorescent proteins delivered by viruses in a format crafted to produce rapid, readily reproducible results. Written in the style of the popular and successful Methods in Molecular Biology(TM) series, the chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.Cutting-edge and easy to use, Viral Applications of Green Fluorescent Protein: Methods and Protocols supplies researchers with an ideal guide to the many uses of GFP and a vital starting point for future studies utilizing this highly adaptable protein. 372 pp. Englisch.…

Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -The significant biological subject, the permeability barrier, is incredibly diverse and vital for a vast assortment of crucial functions in the body. In Permeability Barrier: Methods and Protocols, a variety of experienced researchers contribute techniques to study this complex system in its many forms. Written in the highly successful Methods in Molecular Biology¿ series format, chapters include brief introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Permeability Barrier: Methods and Protocols serves as an ideal guide for all scientists seeking to further our understanding of this vital area of research.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 468 pp. Englisch.…

Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Divided into two convenient sections, Protein Kinase Technologies collects contributions from experts in the field examining recent methodologies and techniques generally applicable to protein kinase research as well as to individual protein kinases which require special attention in neuroscience. These chapters will not only be practical instructions useful for readers' daily work in setting up and performing research but also thought-provoking and enjoyable reviews of recent advancements of individual protein kinases in neuroscience. Written for the highly successful Neuromethods series, this work contains the kind of detailed description and implementation advice that is crucial for getting optimal results.Authoritative and accessible, Protein Kinase Technologies seeks to foster cross-talk among investigators who study different protein kinases, and will also be beneficial for the entry of new investigators into this pivotal field.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 380 pp. Englisch.…

Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Advances in methodologies and experimental models are pivotal to furthering our understanding of central nervous system (CNS) functions in mammals. Isolated Central Nervous System Circuits examines the application a variety of technologies to mostly active in vitro preparations from basically different CNS regions with a diversity of functions. From patch-clamp, multiphoton imaging, live fluorescent protein expression, or immunohistochemistry techniques to acute or 'organotypic' slices and en bloc preparations, this detailed volume features key protocols and examples for exciting results from the labs of renowned experts. Written for the popular Neuromethods series, chapters include the kind of thorough implementation advice that is vital for achieving successful results.Comprehensive and cutting-edge, Isolated Central Nervous System Circuits will greatly aid scientists to further improve models and to develop corresponding modelsof not yet intensively studied structures such as nucleus ruber, superior colliculus, or basal ganglia.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 484 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volume provides an overview of commonly used methods and protocols for cell fitness indicators. Chapters detail biochemical, fluorescence and luminescence-based strategies, computational, and label-free methodologies for assaying cellular viability by means of e.g. viscoelastic properties, impedance and multiphoton microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Cell Viability Assays: Methods and Protocols aims to ensure successful results in the further study of this vital field.Libri GmbH, Europaallee 1, 36244 Bad Hersfeld 316 pp. Englisch.…

Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Mesenchymal Stem Cells have seen an unprecedented level of interest in the last decade, primarily due to their relative ease of isolation, the large numbers of cells present in the adult, and the ability to propagate these cells in culture. In Mesenchymal Stem Cell Assays and Applications, expert researchers from across the globe explore the latest techniques to propagate, characterize, and engineer this special cell type. Chapters outline a set of protocols and assays used by leading investigators in the field, providing standards that can be applied by all researchers to the population of cells used in their experiments. Composed in the highly successful Methods in Molecular Biology¿ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.Ground-breaking and current, Mesenchymal Stem Cell Assays and Applications is a necessary handbook for all researchers working with this ambiguous population of cells.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 552 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volume discusses protocols that aid in measuring different components in the renin-angiotensin-aldosterone system (RAAS). The book also looks at the methods used to assess angiotensin peptides and discerning the influence of RAAS components on different mammalian diseases. The chapters cover topics such as the use of fluorescent substrate to measure ACE2 activity in the mouse abdominal aorta, blood pressure monitoring using the radio telemetry method, and the analysis of angiotensin metabolism in the kidney using mass spectrometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting edge and comprehensive, The Renin-Angiotensin-Aldosterone Systems: Methods and Protocols is a valuable resource that providesscientists and researchers with the best approaches to examine RAAS.Libri GmbH, Europaallee 1, 36244 Bad Hersfeld 216 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volume presents comprehensive laboratory protocols that have been used to generate Th9 cells, both in vitro and in vivo. The techniques described in Th9 Cells: Methods and Protocols study the role of Th9 cells in different inflammatory disease models, including allergic inflammation model, parasite model, tumor model, and EAE and IBD model. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Thorough and comprehensive, Th9 Cells: Methods and Protocols is a valuable resource for scientists in this field and will provide them with techniques to generate Th9 cells for specific downstream events.Libri GmbH, Europaallee 1, 36244 Bad Hersfeld 272 pp. Englisch.…

Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Over the last ten years, Green Fluorescent Proteins, along with the other spectral variants, have emerged from near obscurity to become a powerful and versatile tool in scientific research. In Viral Applications of Green Fluorescent Protein: Methods and Protocols, leading investigators from around the world contribute detailed examples of both the construction and application of fluorescent proteins delivered by viruses in a format crafted to produce rapid, readily reproducible results. Written in the style of the popular and successful Methods in Molecular Biology¿ series, the chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.Cutting-edge and easy to use, Viral Applications of Green Fluorescent Protein: Methods and Protocols supplies researchers with an ideal guide to the many uses of GFP and a vital starting point for future studies utilizing this highly adaptable protein.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 372 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This second edition volume expands on the previous editions with detailed protocols on (cyano) bacterial and mold fungus toxins, and a specific focus on Gram-negative endotoxins (lipopolysaccharides). The chapters in Microbial Toxins: Methods and Protocols, Second Edition present techniques that cover topics such as detection of cholera toxin by an immunochromatographic test strip; label-free immune-sensors for the fast detection of Listeria in food; endotoxin removal from Escherichia coli bacterial lysate using biphasic liquid system; and micromethods for isolation and structural characterization of lipid A, and polysaccharide regions of bacterial lipopolysaccharides. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and practical, Microbial Toxins: Methods and Protocols, Second Edition is a valuable and useful resource for scientists who are interested in this field, and is a great tool for researchers who are looking to learn about new, particular techniques to further enhance their work.Libri GmbH, Europaallee 1, 36244 Bad Hersfeld 216 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volume addresses the various techniques and novel applications of mass spectrometry imaging (MSI) and its role as a discovery tool in the field of proteomics, lipidomics, and metabolomics. The chapters in this book demonstrate how MSI can be applied to many areas of research such as clinical pathology, translational medicine, toxicology, biomarkers and response studies, and potential incorporation of MSI into forensic workflows. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Innovative and comprehensive, Imaging Mass Spectrometry: Methods and Protocols is a valuable resource for research scientists and clinicians who are interested in further studies of MSI technologies.Libri GmbH, Europaallee 1, 36244 Bad Hersfeld 216 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This second edition provides an update on technology and an accelerated tutorial to assist, students, entrepreneurs, new investigators, and established investigators who want to quickly become versed in, and immersed in, the entire process from discovery to clinical trial validation and commercial public benefit. Chapters aim to cover the full spectrum of molecular profiling from tumor staging and grading through biomarker discovery, to commercialization. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Molecular Profiling: Methods and Protocols, Second Edition aims to be a useful guide on molecular profiling.Libri GmbH, Europaallee 1, 36244 Bad Hersfeld 428 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volume is a compilation of sixteen chapters that detail reverse genetics protocols. Reverse Genetics of RNA Viruses: Methods and Protocols guides readers through comprehensive protocols on RNA viruses, that were the most challenging to obtain and/or that were developed most recently. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Reverse Genetics of RNA Viruses: Methods and Protocols aims to ensure successful results in the further study of this vital field.Libri GmbH, Europaallee 1, 36244 Bad Hersfeld 296 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Ataxia-telangiectasia (A-T) is a rare and severe genetic disorder affecting children. A-T is a multisystem disease characterized by progressive neurodegeneration, immunodeficiency and cancer predisposition. This detailed volume explores the ever expanding field of research into the ATM (ataxia-telangiectasia, mutated) gene and the role played by ATM kinase in DNA damage signaling and diverse cellular processes. What follows is a handy desktop reference for both seasoned A-T researchers and postgraduate students, as it demonstrates the breadth of recent developments in A-T studies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Both classic and cutting-edge techniques are described, including ATM gene mutation detection, assays for radiosensitivity and radioresistant DNA synthesis, new methods to measure ATM kinase activity by imaging microscopy and high content screening as well as proteomics, phosphoproteomics and bioinformatics approaches to decipher ATM-dependent signalling pathways. Additional methods include generation of patient-specific stem cells and approaches to study ATM functions in the nervous system.Libri GmbH, Europaallee 1, 36244 Bad Hersfeld 452 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This book is based on selected papers from keynote and symposium sessions given at the 16th International Union of Food Science and Technology (IUFoST) World Congress, held in Foz do Iguaçu, Brazil August, 2012. The theme of the Congress was the challenges faced by food science in both the developed and developing regions of the world. The symposia featured prominent world-renowned keynote and plenary speakers, young researchers, and the technical sessions covered the whole spectrum of basic and applied food science and technology, including consumer issues and education, diets and health, ethnic foods, and R&D.¿Springer-Verlag KG, Sachsenplatz 4-6, 1201 Wien 536 pp. Englisch. …

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volume details PCR technique with focus on its application specificities to the biotechnology and bioengineering field. Chapters are broken into five sections covering sgeneral PCR protocols, different applied examples to molecular and synthetic biotechnology, food science and technology, environmental microbiology and molecular ecology, and healthcare. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, PCR: Methods and Protocols hopes to be a valuable and useful resource for wet lab researchers, particularly within the biotechnology and bioengineering field.Springer-Verlag KG, Sachsenplatz 4-6, 1201 Wien 296 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volume discusses protocols that cover genetic manipulation of Chinese hamster ovary (CHO) cells for recombinant protein production, and protocols for the characterization of CHO cells using ¿omic approaches. This book also explores methods that discuss the genome editing tool, CRISPR/Cas9, and the characterization of recombinant protein products, such as glycosylation and host cell protein analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Heterologous Protein Production in CHO Cells: Methods and Protocols is a valuable resource for scientists and researchers who are interested in further studying cell production in CHO cells.Libri GmbH, Europaallee 1, 36244 Bad Hersfeld 264 pp. Englisch. …

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This thorough volume provides an in-depth introduction to and discussion of microRNAs (miRs) and their targets, miR functions, and computational techniques applied in miR research, thus serving the need for a comprehensive book focusing on miR target genes, miR regulation mechanisms, miR functions performed in various human diseases, and miR databases/knowledgebases. Without prior knowledge of the area of study, computational biologists, computer scientists, bioinformaticians, bench biologists, as well as clinical investigators will find it easy to follow the techniques in this collection. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that ensures successful results.Libri GmbH, Europaallee 1, 36244 Bad Hersfeld 296 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This new edition provides updated and novel protocols of neuroproteomics methods that encompass both global-scale as well as targeted and specialized topics, which are timely additions for the molecular and phenotypic analysis of the central nervous system and CNS-related disorders. The detailed contents of this book include the exploration of several exciting areas of advanced methods used for neuroproteomics research including relative and absolute protein quantitation by mass spectrometry, characterization of post-translational modifications, as well as bioinformatics and computational approaches. Written for the highly successful Methods in Molecular Biology series, methodology chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.Libri GmbH, Europaallee 1, 36244 Bad Hersfeld 444 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This detailed volume provides a toolbox for designing constructs, tackling expression and solubility issues, handling membrane proteins and protein complexes, and exploring innovative engineering of E. coli. The topics are largely grouped under four parts: high-throughput cloning, expression screening, and optimization of expression conditions, protein production and solubility enhancement, case studies to produce challenging proteins and specific protein families, as well as applications of E. coli expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Libri GmbH, Europaallee 1, 36244 Bad Hersfeld 444 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Authored by leading experts from academia, users and manufacturers, this book provides an authoritative account of the science and technology involved in multiparticulate drug delivery systems which offer superior clinical and technical advantages over many other specialized approaches in drug delivery. The book will cover market trends, potential benefits and formulation challenges for various types of multiparticulate systems. Drug solubility, dose, chemistry and therapeutic indications as well as excipient suitability coupled with manufacturing methods will be fully covered. Key approaches for taste-masking, delayed release and extended release of multiparticulates systems are of significant interest, especially their in-vivo and in-vitro performance. In addition, the principles of scale-up, QbD, and regulatory aspects of common materials used in this technology will be explained, as well as recent advances in materials and equipment enabling robust, flexible and cost-effective manufacture. Case studies illustrating best practices will also make the book a valuable resource to pharmaceutical scientists in industry and academia.Springer-Verlag KG, Sachsenplatz 4-6, 1201 Wien 408 pp. Englisch.…

Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Astrocytes were the original neuroglia that Ramón y Cajal visualized in 1913 using a gold sublimate stain. This stain targeted intermediate filaments that we now know consist mainly of glial fibrillary acidic protein, a protein used today as an astrocytic marker. Cajal described the morphological diversity of these cells with some ast- cytes surrounding neurons, while the others are intimately associated with vasculature. We start the book by discussing the heterogeneity of astrocytes using contemporary tools and by calling into question the assumption by classical neuroscience that neurons and glia are derived from distinct pools of progenitor cells. Astrocytes have long been neglected as active participants in intercellular communication and information processing in the central nervous system, in part due to their lack of electrical excitability. The follow up chapters review the ¿nuts and bolts¿ of ast- cytic physiology; astrocytes possess a diverse assortment of ion channels, neu- transmitter receptors, and transport mechanisms that enable the astrocytes to respond to many of the same signals that act on neurons. Since astrocytes can detect chemical transmitters that are released from neurons and can release their own extracellular signals there is an increasing awareness that they play physiological roles in regulating neuronal activity and synaptic transmission. In addition to these physiological roles, it is becoming increasingly recognized that astrocytes play critical roles during pathophysiological states of the nervous system; these states include gliomas, Alexander disease, and epilepsy to mention a few.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 760 pp. Englisch.…