Humana press jul 2011 (19 risultati)

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -In recent years, the need to develop acceptable alternatives to conventional animal testing for neurotoxicity and developmental neurotoxicity has been increasingly recognized, and much effort is being directed toward the development of alternative models, utilizing mostly mammalian cells in culture but also non-mammalian model systems. In Vitro Neurotoxicology: Methods and Protocols presents a series of cellular, biochemical, and molecular methodological protocols in the area of in vitro neurotoxicology, with an emphasis on mammalian cell culture systems. Opening with a section on methodologies for preparing several cellular systems of variable complexity, amenable for in vitro neurotoxicological studies, the thorough volume continues with coverage of methods to measure cellular death and major mechanisms, methods for assessing mechanisms of nervous system cell toxicity related to impairment of cell signaling, while a final section illustrates additional methods for assessing important nervous system processes such as cell proliferation, neuritogenesis, and synaptogenesis. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, In Vitro Neurotoxicology: Methods and Protocols serves researchers with an interest in assessing or characterizing the potential neurotoxicity of environmental contaminants, drugs, or other chemicals. 456 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Since the initial discovery of the claudins, the field of tight junctions and cell polarity has become increasingly energized, mainly due to the fact that the claudins have provided an opportunity to understand the molecular basis of the tight junction function and their tissue specific roles. In Claudins: Methods and Protocols, experts and pioneers in the field provide an extensive collection of techniques involving these tight junction proteins. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include brief introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Claudins: Methods and Protocols serves as an ideal guide for all scientists seeking to further our understanding of this vital area of research. 464 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Recent years have seen significant advancements in the development of enabling technologies that facilitate the study of Transcription Factors (TFs). TFs are pivotal in the regulation of plant development, reproduction, intercellular signaling, response to environment, cell cycle, and metabolism. Plant Transcription Factors: Methods and Protocols offers a comprehensive approach by covering the basic concepts as well as the detailed protocols of a series of commonly used tools for investigating plant TFs. From discussing select TF families in plants to presenting approaches for identifying them, methods are covered to verify the function, to identify protein interactions in which TFs are involved, and how the interactions are mediated. Increasing examples of TFs that function non-cell-autonomously are being discovered and methods to assess intercellular trafficking are also addressed. A section is devoted to examining interaction with DNA, and the volume concludes with a discussion of directed evolution to generate transcription factors that can more efficiently control desired processes. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Plant Transcription Factors: Methods and Protocols serves as an ideal guide to seasoned plant molecular biologists as well as scientists new to the field of TFs and provides many necessary methods to all scientists who are interested in exploring the functions of transcription factors. 360 pp. Englisch. …

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The goal of the characterization and discovery of G protein-coupled receptors, arguably the most important class of signaling molecules in humans and other vertebrates, has spawned numerous vital methodologies. In Methods for the Discovery and Characterization of G Protein-Coupled Receptors, experts in the field present the very latest on the methods and technology used to characterize and discover novel mechanisms of GPCRs which, in many cases, can be used directly to design experiments for the reader's particular GPCR of interest and their specific avenue of investigation. Divided into four convenient sections, this detailed volume covers GPCRs in the genome, trafficking of GPCRs, GPCRs on the membrane, as well as the regulation of these key receptors. Chapters also feature an important section called 'Future Directions' which gives the reader an insight into advances soon to be realized in each area. Written for the popular Neuromethods series, this book contains the kind of detailed description and implementation advice that is crucial for getting optimal results. Authoritative and cutting-edge, Methods for the Discovery and Characterization of G Protein-Coupled Receptors serves as an ideal guide for scientists determined to further our knowledge of crucially important set of receptors. 444 pp. Englisch.…

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Cutting-edge investigators review the current status of the entire field, from the biology of MMPs through the current clinical studies. The authors include many leading scientists from pharmaceutical companies who present all the latest concepts and results on the preferred design strategies for MMP inhibitors, their molecular mechanisms, and their substrates. In addition, they fully describe their personal research on specific MMP inhibitors, detailing vanguard design strategies, their in vitro activity, the outcome of animal model studies and, where available, their toxicology, safety, efficacy in human clinical trials. Comprehensive and state-of-the-art, Matrix Metalloproteinase Inhibitors in Cancer Therapy offers basic and clinical investigators alike a richly informative summary of all the latest research on these powerful new drugs, and their high promise as emerging cancer therapeutics. 280 pp. Englisch.…

Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Cutting-edge investigators review the current status of the entire field, from the biology of MMPs through the current clinical studies. The authors include many leading scientists from pharmaceutical companies who present all the latest concepts and results on the preferred design strategies for MMP inhibitors, their molecular mechanisms, and their substrates. In addition, they fully describe their personal research on specific MMP inhibitors, detailing vanguard design strategies, their in vitro activity, the outcome of animal model studies and, where available, their toxicology, safety, efficacy in human clinical trials.Comprehensive and state-of-the-art, Matrix Metalloproteinase Inhibitors in Cancer Therapy offers basic and clinical investigators alike a richly informative summary of all the latest research on these powerful new drugs, and their high promise as emerging cancer therapeutics.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 280 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -As functional genomics has become one of the major focuses in molecular biology, the need for more sophisticated tools to assist in the identification of the functionality of undefined genes and the correlation of DNA variants with a particular phenotype has increased greatly. In Silico Tools for Gene Discovery collects many common and useful in silico tools available today. The volume begins by investigating locus mapping information on linkage analysis, association mapping, integrative analysis, and exome analysis as well as tools for DNA marker selection, in silico PCR, and statistical analysis. It continues with a section on gene discovery from a defined locus, including gene prioritization, knowledge tracking, and data mining, and concludes with several useful in silico tools presented for the functional characterization of genes, which include DNA sequencing analysis, variant characterization, as well as RNA and protein analysis. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, step-by-step, readily reproducible protocols, and vital notes on troubleshooting and avoiding known pitfalls. Resourceful and easy to follow, In Silico Tools for Gene Discovery seeks to facilitate scientists with further key research on locus mapping, to accelerate gene identification, and to help ascertain the functionality of DNA variation. 380 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Classical methods for microbial strain engineering, used to improve the production of bioproducts, have serious drawbacks and have been found to be unsuitable for complex strain development applications. In Strain Engineering: Methods and Protocols, powerful new genetic engineering-based strain engineering methods are presented for rational modification of a variety of model organisms. These methods are particularly powerful when utilized to manipulate microbes for which sequenced and annotated genomes are available. Collectively, these methods systematically introduce genome alterations in a precise manner, allowing the creation of novel strains carrying only desired genome alterations. In the first section, E. coli-based bacterial strain engineering strategies are reviewed, while the second section presents analogous microbial engineering strategies for eukaryotic cells using the yeast Saccharomyces cerevisiae as a model. The third section covers examples of the proliferative adaptations of these base technologies to strain engineer industrially important prokaryotic or eukaryotic microbial systems. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Strain Engineering: Methods and Protocols serves as an ideal guide to scientists in academia, pharmaceutical science, and biotechnology who perform microbial strain engineering. 492 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Juvenile homicide and fatal maltreatment remain serious and pervasive problems in the developed world and especially in the United States, where in 2005 some 1,500 children died from neglect and physical abuse. Alarming statistics such as this, as well as an upsurge in the media attention paid to all things forensic, underscore the pressing need for the utmost rigor in the scientific investigation of child abuse cases. This well timed volume is a response to the climate of public and press interest in such inquiries, where the forensic aspects of the casework generate an enormous amount of attention. The contributions cover a wide range of topics and explore many of the finer details of investigations into juvenile fatalities suspected of being abuse-related. The chapters reflect both the multi-disciplinary nature of such investigations, and also the need for law enforcement professionals to take a rounded, holistic approach to the casework involved. The motivational factors that lead many professionals enter this arena of investigation are, of course, personal and individual. However, at the core of their commitment and their work is a shared need for justice, plain and simple. Victim advocacy and protecting the rights of children, both living and deceased, remains a key impetus for those professionals who specialize in child abuse research. At the heart of this book is the aim of providing both a vital resource for investigators, and a purposeful voice for the young victims of abuse, unable as they are to stand up and speak for themselves. Juvenile homicide and fatal maltreatment remain serious and pervasive problems in the developed world and especially in the United States, where in 2005 some 1,500 children died from neglect and physical abuse. Alarming statistics such as this, as well as an upsurge in the media attention paid to all things forensic, underscore the pressing need for the utmost rigor in the scientific investigation of child abuse cases. This well timed volume is a response to the climate of public and press interest in such inquiries, where the forensic aspects of the casework generate an enormous amount of attention. The contributions cover a wide range of topics and explore many of the finer details of investigations into juvenile fatalities suspected of being abuse-related. The chapters reflect both the multi-disciplinary nature of such investigations, and also the need for law enforcement professionals to take a rounded, holistic approach to the casework involved. The motivational factors that lead many professionals enter this arena of investigation are, of course, personal and individual. However, at the core of their commitment and their work is a shared need for justice, plain and simple. Victim advocacy and protecting the rights of children, both living and deceased, remains a key impetus for those professionals who specialize in child abuse research. At the heart of this book is the aim of providing both a vital resource for investigators, and a purposeful voice for the young victims of abuse, unable as they are to stand up and speak for themselves. 216 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -To prepare synchronized cells representing different stages of the cell cycle has been a great challenge for researchers across the globe. In Cell Cycle Synchronization: Methods and Protocols, experts in the field supply detailed protocols providing first the theoretical background of the procedure then step-by-step instructions on how to implement synchronization as well as the latest techniques for the enhanced study of regulatory mechanisms to understand cell cycle events. Describing synchronized cells from asynchronous bacterial, plant, protozoan, yeast, fish, and mammalian cell cultures, the synchronization methods presented in the book are based principally on two major strategies: the 'arrest-and-release' approach, which involves different chemical treatments to block cells at certain stages of the cell cycle, and the physical strategy, which contains physical methods to collect cells belonging to subpopulations of the cell cycle. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Detailed and easy to follow, Cell Cycle Synchronization: Methods and Protocols is an ideal guide for scientists who wish to make use of these powerful synchronization techniques but have no access to thorough, time-tested protocols designed for doing so. 308 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Cell migration is a key component of many biological processes including embryonic development, immune responses, wound healing, organ regeneration, and cancer cell metastasis, thus making it an exciting and crucial field of study. The aim of Cell Migration: Developmental Methods and Protocols, Second Edition is to bring together a wide range of these techniques from the more basic migration assays, which are still the foundation of many cell migration studies, to state-of-the-art techniques and recent technical advances. Divided into three convenient parts, the volume begins with a number of basic in vitro migration assays including measurements of wound healing, cell scattering, invasion, and chemotaxis, as well as more complex measurements of transendothelial migration, the use of microfluidic chambers, and imaging cell migration in 3D. It continues with procedures for the imaging and measurement of cell migration in vivo including protocols for the use of chick, drosophila, and zebrafish embryos, and methods to measure metastatic spread and angiogenesis in mice, then concludes with a vital section on emerging techniques in the cell migration field including the use of TIRF, FRAP, and FRET microscopy. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes from the experts on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Cell Migration: Developmental Methods and Protocols, Second Edition provides a comprehensive catalogue of techniques for the study of cell migration that can be used as a useful reference source for any researcher who wishes to explore this significant area of cell biology. 476 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -New research has shown that neuroendocrine tumors are more common than previously thought. Progress in our understanding of the molecular pathogenesis of these not-so-rare cancers has resulted in renewed interest in developing innovative therapeutic options. In particular, novel molecular targeted agents have been shown to have significant anti-tumor activity, and integration of these therapies has led to critical advances in management. In this volume, an outstanding group of the world's leaders in the field have assembled to convey their knowledge of the epidemiology, biology, and management of all the major types of neuroendocrine tumors. The book takes a multi-modality approach to understanding disease processes and therapeutics, including chapters on medical and surgical treatment as well as a chapter devoted to imaging. Throughout, the authors emphasize recent advances in our understanding of molecular biology and the subsequent emerging therapeutic options. 280 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Laser microdissection techniques have revolutionized the ability of researchers in general, and pathologists in particular, to carry out molecular analysis on specific types of normal and diseased cells and to fully utilize the power of current molecular technologies including PCR, microarrays, and proteomics. In second edition of Laser Capture Microdissection: Methods and Protocols, experts in the field provide the reader with practical advice on how to carry out tissue-based laser microdissection successfully in their own laboratory using the different laser microdissection systems that are available and to apply a wide range of molecular technologies. The individual chapters encompass detailed descriptions of the individual laser based micro-dissection systems. The downstream applications of the laser microdissected tissue described in the book include PCR in its many different forms as well as gene expression analysis including application to microarrays and proteomics. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Laser Capture Microdissection: Methods and Protocols, Second Edition is an ideal resource for researchers striving to move forward our understanding of normal physiology and pathology. 512 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Microtubules are at the heart of cellular self-organization, and their dynamic nature allows them to explore the intracellular space and mediate the transport of cargoes from the nucleus to the outer edges of the cell and back. In Microtubule Dynamics: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate microtubule dynamics in vitro and in cells. Beginning with the question of how to analyze microtubule dynamics, the volume continues with detailed descriptions of how to isolate tubulin from different sources and with different posttranslational modifications, methods used to study microtubule dynamics and microtubule interactions in vitro, techniques to investigate the ultrastructure of microtubules and associated proteins, assays to study microtubule nucleation, turnover, and force production in cells, as well as approaches to isolate novel microtubule-associated proteins and their interacting proteins. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Definitive and practical, Microtubule Dynamics: Methods and Protocols provides the key protocols needed by novices and experts on how to perform a broad range of well-established and newly-emerging techniques in this vital field. 332 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Microtubules are at the heart of cellular self-organization, and their dynamic nature allows them to explore the intracellular space and mediate the transport of cargoes from the nucleus to the outer edges of the cell and back. In Microtubule Dynamics: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate microtubule dynamics in vitro and in cells. Beginning with the question of how to analyze microtubule dynamics, the volume continues with detailed descriptions of how to isolate tubulin from different sources and with different posttranslational modifications, methods used to study microtubule dynamics and microtubule interactions in vitro, techniques to investigate the ultrastructure of microtubules and associated proteins, assays to study microtubule nucleation, turnover, and force production in cells, as well as approaches to isolate novel microtubule-associated proteins and their interacting proteins. Written in the highly successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Definitive and practical, Microtubule Dynamics: Methods and Protocols provides the key protocols needed by novices and experts on how to perform a broad range of well-established and newly-emerging techniques in this vital field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 332 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Cell migration is a key component of many biological processes including embryonic development, immune responses, wound healing, organ regeneration, and cancer cell metastasis, thus making it an exciting and crucial field of study. The aim of Cell Migration: Developmental Methods and Protocols, Second Edition is to bring together a wide range of these techniques from the more basic migration assays, which are still the foundation of many cell migration studies, to state-of-the-art techniques and recent technical advances. Divided into three convenient parts, the volume begins with a number of basic in vitro migration assays including measurements of wound healing, cell scattering, invasion, and chemotaxis, as well as more complex measurements of transendothelial migration, the use of microfluidic chambers, and imaging cell migration in 3D. It continues with procedures for the imaging and measurement of cell migration in vivo including protocols for the use of chick, drosophila, and zebrafish embryos, and methods to measure metastatic spread and angiogenesis in mice, then concludes with a vital section on emerging techniques in the cell migration field including the use of TIRF, FRAP, and FRET microscopy. Written in the highly successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes from the experts on troubleshooting and avoiding known pitfalls.Comprehensive and up-to-date, Cell Migration: Developmental Methods and Protocols, Second Edition provides a comprehensive catalogue of techniques for the study of cell migration that can be used as a useful reference source for anyresearcher who wishes to explore this significant area of cell biology.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 476 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Juvenile homicide and fatal maltreatment remain serious and pervasive problems in the developed world and especially in the United States, where in 2005 some 1,500 children died from neglect and physical abuse. Alarming statistics such as this, as well as an upsurge in the media attention paid to all things forensic, underscore the pressing need for the utmost rigor in the scientific investigation of child abuse cases. This well timed volume is a response to the climate of public and press interest in such inquiries, where the forensic aspects of the casework generate an enormous amount of attention. The contributions cover a wide range of topics and explore many of the finer details of investigations into juvenile fatalities suspected of being abuse-related. The chapters reflect both the multi-disciplinary nature of such investigations, and also the need for law enforcement professionals to take a rounded, holistic approach to the casework involved. The motivational factors that lead many professionals enter this arena of investigation are, of course, personal and individual. However, at the core of their commitment and their work is a shared need for justice, plain and simple. Victim advocacy and protecting the rights of children, both living and deceased, remains a key impetus for those professionals who specialize in child abuse research. At the heart of this book is the aim of providing both a vital resource for investigators, and a purposeful voice for the young victims of abuse, unable as they are to stand up and speak for themselves.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 216 pp. Englisch. …

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The Atlas of Robotic Urologic Surgery provides a detailed, step-by-step guide to common robotic urologic procedures for the purpose of helping novice surgeons in their transition to robotic surgery and seasoned robotic surgeons to refine their surgical technique and expand their repertoire of robotic procedures. In addition, less commonly performed robotic procedures such as those for male infertility, pelvic organ prolapse, urinary tract reconstruction and pediatrics are included. Each chapter is written by the thought leaders in robotic urologic surgery with descriptive step-by-step text, complimented by figures and intraoperative photographs detailing the nuances of each procedure. Emphasis is placed on operative setup, instrument and equipment needs and surgical techniques for both the primary surgeon as well as the operative assistant. The use of ancillary equipment and robotic instrument and endoscope exchanges are highlighted throughout the procedural text by tables designed to aid surgeons and their teams in improving efficiency. This volume will provide unique insights into robotic urologic surgery and reduce the learning curve of accomplishing these increasingly popular procedures. 288 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -The Atlas of Robotic Urologic Surgery provides a detailed, step-by-step guide to common robotic urologic procedures for the purpose of helping novice surgeons in their transition to robotic surgery and seasoned robotic surgeons to refine their surgical technique and expand their repertoire of robotic procedures. In addition, less commonly performed robotic procedures such as those for male infertility, pelvic organ prolapse, urinary tract reconstruction and pediatrics are included. Each chapter is written by the thought leaders in robotic urologic surgery with descriptive step-by-step text, complimented by figures and intraoperative photographs detailing the nuances of each procedure. Emphasis is placed on operative setup, instrument and equipment needs and surgical techniques for both the primary surgeon as well as the operative assistant. The use of ancillary equipment and robotic instrument and endoscope exchanges are highlighted throughout the procedural text by tables designed to aid surgeons and their teams in improving efficiency. This volume will provide unique insights into robotic urologic surgery and reduce the learning curve of accomplishing these increasingly popular procedures.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 288 pp. Englisch.…