Humana press sep 2012 (42 risultati)

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This special book is for every woman who has ever been pregnant, or is now experiencing her first pregnancy, as well as all those hoping to better understand pregnancy's powerful feelings and events-the joy, the pain, the wonder, the bonding, the miracle of new life! 124 pp. Englisch.…

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -In this commonsense book, Dr. Gershwin and Dr. Klingelhofer offer sensible, practical strategies for identifying the substances that cause allergies (allergens), the symptoms they cause, ways exposure to allergens can be avoided or reduced, and ways you can relieve the symptoms. Living Allergy Free also treats some reactions that are commonly assumed to be allergies, but are really sensitivity or irritant reactions, shows how they can be distinguished from true allergies, and tells you what to do about them. If you suffer from allergies-or if you think you do-Living Allergy Free is just what the doctor would order! 316 pp. Englisch.…

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -In When Medicine Went Mad, one of the nation's leading bioethicists-and an extraordinary panel of experts and concentration camp survivors-examine problems first raised by Nazi medical experimentation that remain difficult and relevant even today. The importance of these issues to contemporary bioethical disputes-particularly in the thorny areas of medical genetics, human experimentation, and euthanasia-are explored in detail and with sensitivity. 376 pp. Englisch.…

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -In Alcohol, Cocaine, and Accidents leading experts provide concise yet focused scientific reviews of the role of alcohol and cocaine in automotive, aviation, and aquatic accidents. The authoritative contributors present both applied research and epidemiological studies, with emphasis on the prevention of injuries through an increased use of appropriate educational labels, law enforcement, and personal and community-based prevention programs. In addition to detailed topical coverage, the articles suggest prevention strategies and provide supporting data for the role of treatment in reducing accidents.Alcohol, Cocaine, and Accidents will be of interest to substance abuse researchers, law enforcement officials, treatment providers, policy makers, and legislators responsible for regulating alcohol and drug use, as well as safety issues in all areas of transportation. 228 pp. Englisch.…

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book grew out ofmy interest in what is often called 'the immunological paradox ofpregnancy.' How is it possible that the fetus-halfofwhosegenetic apparatuscomesfrom thefather and is foreign to the mother-can survive to term This is a question that intrigues all immunologists. For me, it has been of interest ever since I heard a lecture on the subject in medical school, long before I thought ofbecoming a 'professional immunologist.' Indeed, the question ofthe immunological aspect of fetal survival (or demise) should be of interest to any biologist or physician. The question becomes broader ifone considers the immunologic relations between motherand fetus, because they represent a unique symbiotic union. Whatimmunologic problemsinthemothermayaffecttheoffspring, and isitpossiblethatfetal immunology willaffectthe mother Finally, there is the question ofwhether immunology is important in recur rent spontaneous abortion. Every authorowes the reader a general oversightofthe book in hand, indicating the terrain to be covered, and, by inference, the territory that will not be explored. 1. This is primarily a book for clinicians. I will only men tion animal experiments and data in passing, and as they may illuminate a clinical problem or observation. 2. The interest here is the immunology ofmaterno--fetal re lations, once a pregnancy has begun. Therefore, I will notcover immunological aspects ofsterility, nor touch on the immunological approaches to controlling fer tility, i.e., 'contraceptive vaccines.' 3. This is a book mainly concerned with pathogenesis. 236 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The genomes of cellular organisms are organized as double-stranded DNA, a structure that must be unwound to provide DNA replication, recombination, and repair machinery access to genomic information. However, DNA unwinding comes with inherent risks to genome stability. To help mediate these risks, bacterial, archael, and eukaryotic cells have evolved protective ssDNA-binding proteins (SSBs) that bind ssDNA with high affinity and specificity. SSBs also aid genome metabolic processes through direct interactions with key proteins in genome maintenance enzymes. Single-Stranded DNA Binding Proteins: Methods and Protocols assembles methods developed for examining the fundamental properties of SSBs and for exploiting the biochemical functions of SSBs for their use as in vitro and in vivo reagents. Clearly and concisely organized, the volume opens with an introduction to the structures and functions of SSBs, followed protocols for studying SSB/DNA complexes, methods for studying SSB/heterologous protein complexes, protocols for interrogating post-translational modifications of SSBs, and concludes with uses of fluorescently-labeled SSBs for in vitro and in vivo studies of genome maintenance processes. Written in the successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Single-Stranded DNA Binding Proteins: Methods and Protocols provides a rich introduction for investigators who are interested in this fascinating family of DNA-binding proteins. 272 pp. Englisch. …

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The regulation of intracellular Ca2+ has continued to be a powerful area of study since the publication of the first and second editions of Calcium Signaling Protocols, and the developments in the field have also, naturally, continued. With the third edition, expert contributors explore some of the exciting new molecular techniques that have both enabled new studies of intracellular Ca2+ regulation and provided much new information on processes. Comprised of five main section, the book covers theoretical and very simple suspension-based fluorimetric assays, specialist measurement systems, measurement of channel activity, measurement of store release, as well as specialist measurement techniques which include targeted probes, using G-protein chimeras to force Ca2+ signalling for screening, and genetically encoded sensors. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Updated and accessible, Calcium Signaling Protocols, Third Edition will be a valuable reference for all those contemplating a move into the study of intracellular Ca2+. 372 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The field of nanotechnology has developed very rapidly over the past decade lending great promise to medical applications in drug delivery, therapeutics, and biological imaging. Due to the great promise, rapid development, and broad application of nanomaterials, it is imperative that researchers from development through application seek a thorough understanding of nanotoxicity. Nanotoxicity: Methods and Protocols address the special considerations when applying toxicity studies to nanomaterials and detail newly developed methods for the study of nanotoxicity. These diverse methods span in vitro cell culture, model tissues, in situ exposure, in vivo models, analysis in plants, and mathematical modeling, proving to be relevant to pharmaceutical scientists, material scientists, bioengineers, toxicologists, environmentalists, immunologists, and cellular and molecular biologists, to name a few. As part of the highly successful Methods in Molecular Biology(TM), chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Nanotoxicity: Methods and Protocols aims to diversify the capabilities of current researchers involved in nanotoxicology and to enable researchers in related fields to expand their knowledge of how nanomaterials interface with the biological environment. 428 pp. Englisch. …

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Over the past three decades there have been new developments in therapeutic drug design. In Rational Drug Design: Methods and Protocols, expert researchers in the field detail many of the methodologies used to study rational drug design. These include methods such as virtual screening of chemical hits, rational lead discovery by high throughput screening, combinatorial and fragment based lead generation, peptide based drug discovery, and animal models of lead validation. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Rational Drug Design: Methods and Protocols seeks to aid scientists in the further study of rational drug design and future drug discovery. 244 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Membrane proteins play key roles in numerous cellular processes, in particular mediating cell-to-cell communication and signaling events that lead to a multitude of biological effects. Membrane proteins have also been implicated in many critical diseases such as atherosclerosis, hypertension, diabetes and cancer. In Membrane Protein Structure Predictions Methods: Methods and Protocols, expert researcher in the field detail the advances in both experimental and computational approaches of the structure, dynamics and interactions of membrane proteins dividing the volume into two sections. The first section details the procedures used for measurements of structure and dynamics of membrane proteins. While the second section contains a survey of the computational methods that have played a critical role in membrane protein structure prediction as well as in providing atomic level insight into the mechanism of the dynamics of membrane receptors. Written in the highly successful Methods in Molecular Biology(TM) series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.Thorough and intuitive, Membrane Protein Structure Predicitons: Methods and Protocols seeks to aid scientists in the further study of membrane protein structure and function. 372 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Capillary electrophoresis (CE) is a powerful and rapid tool for performing complex analyses of a number of different molecular species ranging from small inorganic ions to large nucleic acid fragments and proteins. It is quickly becoming established as a useful tool in clinical medicine due to its consumption of minute samples (less than a microlitre), low reagent costs, and extreme sensitivity, depending upon the source of detection used. Clinical Applications of Capillary Electrophoresis aims to give an in-depth manual of CE applications in several important areas of clinical science. Divided into seven sections, this volume provides a brief overview of how CE has been applied in clinical settings, followed by several chapters on CE analysis of important diagnostic molecules and biofluids, as well as descriptions of applications in clinical chemistry, hematology, bacteriology, virology, disease-associated biomarker discovery, immunology and genetic analysis. Written in the successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Clinical Applications of Capillary Electrophoresis seeks to serve as a valuable source of information not only for clinical pathologists, but also clinical scientists who wish to apply the technique to diagnosis and research. 328 pp. Englisch.…

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -A major national goal is to improve our health and advance our opportunities to pursue happiness. Simulta neously, there are increasing health care costs and increasing demands to accomplish more with less financial support. Treatment costs can be reduced and health improved by preventing the toxic effects of drugs. This first volume of our new series, Drug and Alcohol to reduce the use and Abuse Reviews, focuses on stategies abuse of common compounds known to cause major damage to health: alcohol, tobacco, and illicit drugs such as cocaine and heroin. With the number of deaths attributable to the consumption of alcohol in the US at about 100,000 per year, the annual cost of addictions will be $150 billion by 1995. A variety of approaches to preventing drug abuse are being applied by governmental agencies and health care providers to reduce costs. These include school-based inter ventions, driver education programs, media interventions, health warning labels, physician guidance, economic dis incentives, restricted availability, punishments and penalties, environmental protections, and social-support approaches. With such a range of options, it becomes critical to evaluate and choose the most effective systems for a given population. We feel that the present collection of critical survey articles constitutes a thorough examination of the issues and strategies associated with prevention, and trust that readers will find the book exceedingly helpful in under standing and planning what needs to be done. 420 pp. Englisch.…

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -New and exciting biological functions are still being discovered for vitamin A derivatives, including the vast number of physiological activities of retinoids. In Retinoids: Methods and Protocols, expert researchers in the field present the most recent technical tools with diverse techniques for both in vitro and in vivo studies. Combining biochemical, biophysical, and cell biological techniques, the book addresses topics such as the detection and quantitation of retinoids using HPLC, mass spectrometry, and fluorescence, fluorescence anisotropy of retinol binding protein, cell culture models for studying retinoid transport and the role of retinol in embryonic stem cell culture, as well as many other detailed procedures. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Retinoids: Methods and Protocols seeks to aid beginning and experienced researchers from widely varied fields in the search to uncover even more vital aspects of vitamin A's impact on the human body. 376 pp. Englisch. …

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Maurie Markman and a panel of distinguished clinicians and leading clinical investigators comprehensively review the current status of regional antineoplastic drug delivery in the management of malignant disease. These authorities present a critical analysis of both the rationale and limitations of regional therapy and discuss potential clinical trials designed to explain the effectiveness of this method of therapy in special settings. Their presentations describe many exciting and innovative strategies for using regional drug delivery in anticancer therapy, including coverage of such areas of special interest as colorectal, skin, lung, pancreatic, ovarian, and gastrointestinal cancers. Comprehensive and authoritative, Regional Chemotherapy: Clinical Research and Practice offers surgical and medical oncologists and clinical cancer investigators a gold-standard review of the current role and future development of this increasingly powerful weapon in the battle against cancer. 376 pp. Englisch.…

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The preparation of Aging, Immunity, and Infection has been a 'labor of labor. ' When we began, there existed a huge literature-but manage able, we thought, given our years of experience in the area often referred to as immunogerontology. However, in the time that we have been at work, the new relevant literature has increased at a prodigious rate. The more we read and tried to assimilate, the farther we fell behind. In order to have any hope of completing a book on this rapidly evolving topic, we have been forced to become increasingly selective in covering new and re cent publications. We dare to hope that many read ers will find the book useful and only a few will dweIl on the inevitable inadequacies. We consider the book a work in progress, and welcome suggestions for future editions. Five chapters cover several aspects of infection and the decline of immunity with age. The first chapter 'Human Aging: Present and Future,' is devoted to demographics and theories of senescence. Chap ter 2 outlines the gradual breakdown of resistance to infection in the aged individual. Chapters 3 and 4 cover changes in innate and acquired immunity. The final chapter, 'Nutrition, Longevity, and Integrity of the Immune System,' discusses such provocative ideas as life-span exten sion and nutritional intervention for the delay of immunosenescence. 252 pp. Englisch.…

Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Focusing on the rapidly increasing interaction between biotechnology and advanced fiberoptics/electronics, Biosensors with Fiberoptics emphasizes the three major phases of the developmental process from concept to marketplace: research, development, and applications.With contributions by leading experts directly involved in key areas of this exciting, fast-paced field, the book describes novel biosensor technologies and such current and potential applications as: chemical sensing ¿ fluorescent labels ¿ evanescent-wave biosensors and immunosensors ¿ clinical applications ¿ immunoassay kinetics ¿ luminescence ¿ monitoring of environmental toxins.Researchers and clinicians in a broad spectrum of disciplines will find Wise and Wingard's Biosensors with FIBEROPTICS an extremely practical, up-to-date reference text.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 388 pp. Englisch.…

Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -The preparation of Aging, Immunity, and Infection has been a 'labor of labor. ' When we began, there existed a huge literature-but manage able, we thought, given our years of experience in the area often referred to as immunogerontology. However, in the time that we have been at work, the new relevant literature has increased at a prodigious rate. The more we read and tried to assimilate, the farther we fell behind. In order to have any hope of completing a book on this rapidly evolving topic, we have been forced to become increasingly selective in covering new and re cent publications. We dare to hope that many read ers will find the book useful and only a few will dweIl on the inevitable inadequacies. We consider the book a work in progress, and welcome suggestions for future editions. Five chapters cover several aspects of infection and the decline of immunity with age. The first chapter 'Human Aging: Present and Future,' is devoted to demographics and theories of senescence. Chap ter 2 outlines the gradual breakdown of resistance to infection in the aged individual. Chapters 3 and 4 cover changes in innate and acquired immunity. The final chapter, 'Nutrition, Longevity, and Integrity of the Immune System,' discusses such provocative ideas as life-span exten sion and nutritional intervention for the delay of immunosenescence.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 252 pp. Englisch.…

Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -A major national goal is to improve our health and advance our opportunities to pursue happiness. Simulta neously, there are increasing health care costs and increasing demands to accomplish more with less financial support. Treatment costs can be reduced and health improved by preventing the toxic effects of drugs. This first volume of our new series, Drug and Alcohol to reduce the use and Abuse Reviews, focuses on stategies abuse of common compounds known to cause major damage to health: alcohol, tobacco, and illicit drugs such as cocaine and heroin. With the number of deaths attributable to the consumption of alcohol in the US at about 100,000 per year, the annual cost of addictions will be $150 billion by 1995. A variety of approaches to preventing drug abuse are being applied by governmental agencies and health care providers to reduce costs. These include school-based inter ventions, driver education programs, media interventions, health warning labels, physician guidance, economic dis incentives, restricted availability, punishments and penalties, environmental protections, and social-support approaches. With such a range of options, it becomes critical to evaluate and choose the most effective systems for a given population. We feel that the present collection of critical survey articles constitutes a thorough examination of the issues and strategies associated with prevention, and trust that readers will find the book exceedingly helpful in under standing and planning what needs to be done.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 420 pp. Englisch.…

Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -In Alcohol, Cocaine, and Accidents leading experts provide concise yet focused scientific reviews of the role of alcohol and cocaine in automotive, aviation, and aquatic accidents. The authoritative contributors present both applied research and epidemiological studies, with emphasis on the prevention of injuries through an increased use of appropriate educational labels, law enforcement, and personal and community-based prevention programs. In addition to detailed topical coverage, the articles suggest prevention strategies and provide supporting data for the role of treatment in reducing accidents.Alcohol, Cocaine, and Accidents will be of interest to substance abuse researchers, law enforcement officials, treatment providers, policy makers, and legislators responsible for regulating alcohol and drug use, as well as safety issues in all areas of transportation.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 228 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Super secondary structure(SSS) helps to understand the relationship between primary and tertiary structure of proteins. In Protein Supersecondary Structure: Methods and Protocols expert researchers in the field detail the usefulness of the study of super secondary structure in different areas of protein research. This is done through four main studies SSS representation, SSS prediction, SSS and protein folding, and other application of SSS concept to protein biology. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein Supersecondary Structure: Methods and Protocols highlight some of the major advances in the many fast-growing areas of supersecondary structure research. 340 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The growth of the field of eating disorder research has led to a vast array of empirical articles, and the development of new animal models that can be used to study these disorders continues to stimulate new research. Animal Models of Eating Disorders serves as a collection of detailed techniques contributed by experts in the field who are well-versed in the development and implementation of these models. Since eating disorders are complex and likely due to a combination of environmental, genetic, and social causes, the detailed chapters of this volume have been designed to highlight different contributing factors. Collectively, these chapters give a comprehensive and representative overview of both recently developed and classic methodologies used in the study of eating disorders. Written for the popular Neuromethods series, this work contains the kind of thorough description and implementation advice that promises successful results. Authoritative and practical, Animal Models of Eating Disorders aims to aid researchers in the use of animal models to assist in their investigation and characterization of the behaviors and neurochemical alterations associated with these devastating disorders. 396 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Current knowledge of the mechanisms that regulate DNA repair has grown significantly over the past years with technology advances such as RNA interference, advanced proteomics and microscopy as well as high throughput screens. The third edition of DNA Repair Protocols covers various aspects of the eukaryotic response to genomic insult including recent advanced protocols as well as standard techniques used in the field of DNA repair. Both mammalian and non-mammalian model organisms are covered in the book, and many of the techniques can be applied with only minor modifications to other systems than the one described. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Thorough and intuitive, DNA Repair Protocols, Third Edition provides expert guidance for DNA repair, recombination, and replication. 652 pp. Englisch.…

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -In a rapidly evolving and extremely important area of medical science, it is often difficult for the student, teacher, and researcher to keep abreast of all the important advances. The purpose of Molecular Biology ofDiabetes, Parts I and II is to bring to these individuals the latest knowledge of diabetes-related research in a comprehensive, yet concise manner. To this end, we have assembled chapters, written by most of the world's experts in the field, that we believe compre hensively survey and synthesize a coherent understanding of the subject. Studies of the etiology of type I and type II diabetes are extremely exciting and essential, since we hope to one day prevent the disease using gene therapy. These aspects are covered in Molecular Biology of Diabetes: I. Autoimmunity and Genetics; Insulin Synthesis and Secretion. In type II diabetes, an abnormality in pancreatic secretion exists concomitantly with peripheral insulin resistance. This abnor mality of insulin secretion is believed to be related to a defect(s) in glucose sensing. Uncoupling of glucose sensing from insulin secre tion may be the crucial step in the pathogenesis of noninsulin-depen dent diabetes. In this volume, we have invited authors to describe their studies on all known factors affecting ~-cell function, including autoimmunity and genetics of diabetes, as well as molecular mecha nisms of insulin synthesis and secretion. In the last few years, the most rapidly advancing area of research in diabetes has been, in fact, related to insulin action. 428 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Phytoplasmas are a group of bacteria that are capable of multiplying and causing severe diseases in a wide range of plant species and are transported between plants by insect vectors in which the bacteria can also multiply. Phytoplasmas can cause serious and devastating problems for crop plants, especially in developing countries. In Phytoplasmas: Methods and Protocols experts in the field cover topics pertinent to scientists intending to develop a research program on phytoplasmas. The volume opens with an introductory chapter on the importance of phytoplasma diseases, followed by methods for detection and diagnosis as well as techniques for separating and classifying the phytoplasmas into their different taxonomic groups and subgroups. The final chapters cover methods for separating phytoplasma genomic and plasmid DNA from plant DNA for whole genome sequencing. Written in the successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Phytoplasmas: Methods and Protocols serves as an ideal guide to plant pathologists and molecular biologists aiming to set up a diagnostics facility for identifying the presence of these pathogens in plants or their insect vectors. 436 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Extensive studies have been conducted on the identification, biogenesis, and processing of microRNA (miRNA) as well as research on the exact mechanism by which miRNAs bring about translational silencing of their targets. In addition, numerous publications point to an important role of miRNAs in development, reprogramming, epigenetics, pathogenesis of cancer, oncogenes and tumor suppressors, biomarkers of various disease onset, and regulation of adipogenesis and obesity; yet many questions still remain. In MicroRNA Protocols, Second Edition, experts in the field provide up-to-date coverage of this diverse area of study. The specific chapters of this edition are related to the analysis of miRNA, targets and expression profiling, various methods to determine its regulation of gene expression, the preparation and isolation of miRNAs in specific tissues, its detection in the saliva, and potential application in cosmetics, wound healing, and prostate cancer. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and known pitfalls. Fully updated and easy to use, MicroRNA Protocols, Second Edition aims to stimulate readers to explore diverse ways to understand the mechanisms in which miRNAs facilitate the molecular aspects of not only biomedical research but also a wide range of other research fields. 388 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Renal Cell Carcinoma: Clinical Management provides a comprehensive, state-of-the art review of this field, and will serve as a valuable resource for clinicians, surgeons and researchers with an interest in kidney cancer. The volume reviews new data about risk factors for the disease, profiles the new staging system for renal cell carcinoma (RCC), highlights our current understanding of familial syndromes of RCC and their molecular genetics, and provides new perspectives about imaging of renal tumors. For localized disease, a greater appreciation of nephron-sparing approaches and the importance of preservation of renal function is discussed in detail. Advances in surgical techniques are highlighted. For metastatic disease, the molecular biology of kidney cancer is reviewed in a concise and understandable manner, including the data that has fostered novel targeted approaches that have revolutionized management. The several landmark phase III targeted therapy trials published in the last few years are placed in context with respect to current management. Integration of surgery and systemic therapy through cytoreductive and consolidative approaches has yielded provocative data that promises to further advance the field, and major advances in this domain are also be discussed. 376 pp. Englisch.…

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Itisonlyrecently thatthe naturaloccurrenceoffree radicalsin biological tissue has become widely accepted, and that the suspi cion with which biologists previously viewed the free radicals of radiationchemistryhas beenplacedin a broaderperspective. Now, oxygen-derived free radicals are considered respectable biochemi cal intermediates, given always the caveat that unwanted tissue damage may arise if these active species are produced in such abundance that they overwhelm the natural antioxidant and free radical defense mechanisms, or if these systems have become hypoeffective. Many factors, including several dietary manipula tions, can lead toelevatedproductionofsuperoxide and may result in free radical overload, whereas a deficiency of those micronutri ents associated with the antioxidant defense mec.hanisms may re sult in substantially diminished antioxidant capacity. By now, antioxidants have become a household word and al most everyone is aware of their imponance in protecting the body against attack by active oxygen species. Indeed, it is a paradox of nature that oxygen, which is so essential to sustain aerobic life, ul timately contributes to its destruction. Not surprisingly, recogni tion ofthis dilemma has generated a spate ofantioxidant strategies intended to reduce the risk of tissue damage by rampant oxygen radicals, some sadly based less on science than on speculation. 244 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Identifying Helicobacter infection as the leading cause of peptic ulcer disease and gastric cancer has dramatically altered the treatment of these disease states. Over the last several decades, scientists have come to understand that the interplay between the bacteria, the host, and the environment all contribute to the clinical outcome of infection. In Helicobacter Species: Methods and Protocols, expert researchers in the field detail many of the methods and which are now commonly used to study Helicobacter infection. These include protocols and methods that have evolved over time, and standards across the field have been established which are essential for optimal outcomes and to allow comparison of data across different laboratories. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Helicobacter Species: Methods and Protocols seeks to aid scientists in further study of this crucially important research into Helicobacter research. 268 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Over the past ten years, many powerful new techniques have been developed that have dramatically changed malaria research. The second edition of Malaria: Methods and Protocols expands upon the previous edition with current, detailed techniques for laboratory research. With new chapters on parasite culture techniques, genome manipulation methods, 'omic' approaches, and techniques for studying the biology of the red blood cell and pre-erythrocytic stages of Plasmodium. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Malaria: Methods and Protocols, Second Edition, offers a comprehensive set of standard techniques for laboratory researchers. 644 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Magnesium is an essential mineral which is required for growth and survival of humans. Since magnesium is a mineral and not synthesizable it must be obtained through dietary foods and/or supplements. Magnesium in Human Health and Disease reviews the benefits of magnesium supplementation to reach recommended intakes as well as provides new research that suggests how reaching levels above the recommended intakes can promote health and treat various diseases. Magnesium deficiency can cause low serum potassium and calcium levels, retention of sodium, and low circulating levels of regulatory hormones. These changes in nutrients cause neurological and muscular symptoms such as tremor and muscle spasms. Further magnesium deficiency causes loss of appetite, nausea, vomiting, personality changes and death from heart failure. Causes of magnesium deficiency include alcohol abuse, poorly controlled diabetes, excessive or chronic vomiting and/or diarrhea. Thus the effects of inadequate and deficient intakes or levels of magnesium is critical to health and are reviewed by the expert clinicians in this book. Magnesium in Human Health and Disease provides the most current research to support the potential benefits or lack thereof for normal and high supplementation with magnesium. Animal model research and early human trials are reviewed to document other disease states such as hypertension, cholesterol level, type 2 diabetes and cardiovascular disease that would benefit from increased magnesium. 332 pp. Englisch. …