Springer nature apr 1992 (11 risultati)

Buch. Condizione: Neu. Neuware - 1 The baculoviruses.- 1.1 Introduction.- 1.2 Isolation and host range.- 1.3 Structure and classification.- 1.4 Baculovirus replication in vivo.- 1.5 Baculovirus replication in vitro.- 1.5.1 Baculovirus gene expression and replication.- 1.5.2 Baculovirus gene promoters.- 1.6 Genetic engineering of baculovirus insecticides.- 2 The development of baculovirus expression vectors.- 2.1 Introduction and historical perspective.- 2.2 The merits of the baculovirus expression system.- 2.2.1 Advantages.- 2.2.2 Disadvantages.- 2.3 General principles for inserting foreign genes into the baculovirus genome.- 2.4 Baculovirus transfer vectors.- 2.4.1 Polyhedrin promoter-based expression vectors.- 2.4.2 p10 promoter-based transfer vectors.- 2.4.3 Multiple expression vectors.- 2.4.4 Transfer vectors utilizing other baculovirus gene promoters.- 2.5 Selection of recombinant viruses.- 2.5.1 Selection of a polyhedrin-negative phenotype.- 2.5.2 Selection of f3-galactosidase-negative viruses.- 2.5.3 Recombinant virus selection using dot-blot hybridization.- 2.5.4 Screening for a positive phenotype.- 2.5.5 Enhancing the numbers of recombinant viruses.- 3 Processing of foreign proteins synthesized using baculovirus vectors in insect cells.- 3.1 Introduction.- 3.2 Glycosylation.- 3.3 Phosphorylation, acylation and amidation.- 3.4 Proteolytic processing.- 3.5 Cellular targeting and secretion.- 3.6 Tertiary and quaternary structure formation.- 3.7 Expression of viral genes.- 3.8 Expression of bacterial and fungal genes.- 3.9 Post-transcriptional processing.- 4 Construction of transfer vectors containing the foreign gene.- 4.1 Introduction.- 4.2 Isolation of foreign gene coding sequences.- 4.2.1 Some general guidelines.- 4.2.2 Isolation of DNA fragments from agarose gels.- 4.3 Modifying the ends of DNA molecules.- 4.3.1 Mung bean nuclease.- 4.3.2 Klenow fill-in.- 4.4 Preparation of the transfer vector.- 4.5 DNA ligations.- 4.6 Transformation of bacteria.- 4.7 Screening for recombinant baculovirus transfer vectors.- 4.7.1 Colony hybridization.- 4.7.2 Rapid isolation of bacterial plasmid DNA (mini-preps).- 4.8 Analysis of recombinant transfer vectors.- 4.9 Isolation of highly purified plasmid DNA (maxi-preps).- 5 Insect cell culture media and maintenance of insect cell lines.- 5.1 Introduction.- 5.2 Cell lines.- 5.3 Culture media.- 5.4 Preparation of culture media.- 5.4.1 Preparation of TC100/FCS growth medium.- 5.4.2 Preparation of Grace's (TNM-FH) growth medium.- 5.4.3 Preparation of 4.5 1 TC100 medium from powdered formula.- 5.4.4. Preparation of TC100 medium from individual ingredients.- 5.4.5 Specialized TC100 media.- 5.4.6 Alternative insect cell culture media.- 5.5 Glassware and disposable plasticware.- 5.5.1 Suggested cleaning regime for tissue culture glassware.- 5.6 Insect cell culture.- 5.6.1 Routine sub-culturing of Sf cell lines (monolayer cultures).- 5.6.2 Routine sub-culturing of Sf cells maintained in spinner cultures.- 5.7 A guide to Sf cell seeding densities for experimental work.- 5.8 Freezing, storage and recovery of insect cells in liquid nitrogen.- 5.8.1 Freezing and storage of cells in liquid nitrogen.- 5.8.2 Recovery of cells from liquid nitrogen.- 5.9 A guide to adapting cells to serum-free media.- 6 Propagation, titration and purification of AcMNPV in cell culture.- 6.1 Introduction.- 6.1.1 Safety considerations: general rules for working with baculoviruses.- 6.2 Infection of cells with virus for experimental work.- 6.2.1 Infection of Sf cells in monolayer culture.- 6.2.2 Infection of Sf cells in suspension culture.- 6.3 Titration of virus by plaque-assay.- 6.3.1 Standard plaque-assay.- 6.3.2 Plaque-assay of lacZ-positive viruses.- 6.4 Plaque-picking and plaque-purification.- 6.5 Amplification of virus stocks.- 6.5.1 To prepare a seed stock of virus from a plaque-pick.- 6.5.2 Preparation of an intermediate stock of virus.- 6.5.3 Preparation of a high-titre working stock of virus.- 6.6 Large-scale production of virus for the.…

Buch. Condizione: Neu. Neuware - 1 Descriptive Statistical Techniques.- 2 Environmetric Methods of Nonstationary Time-Series Analysis: Univaraute Methods.- 3 Regression and Correlation.- 4 Factor and Correlation Analysis of Multivariate Environmental Data.- 5 Errors and Detection Limits.- 6 Visual Representation of Data Including Graphical Exploratory Data Analysis.- 7 Quality Assurance for Environmental Assessment Activities.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Erwin Schrödinger is one of the greatest figures of theoretical physics, but there is another side to the man: not only did his work revolutionize physics, it also radiacally changed the foundations of our modern worldview, modern biology, philosophy of science, philosophy of the mind, and epistemology. This book explores the lesser-known aspects of Schrödinger's thought, revealing the physicist as a philosopher and polymath whose highly original ideas anticipated the current merging of the natural and the social sciences and the humanities. Thirteen renowned scientists and philosophers have contributed to the volume. Part I reveals the philosophical importance of Schrödinger's work as a physicist. Part II examines his theory of life and of the self-organization of matter. Part III shows how Schrödinger's ideas have influenced contemporary philosophy of nature and our modern view of the world, drawing a fascinating picture of the ongoing synthesis of nature and culture: one of the most interesting developments of modern thought. The volume also contains the most comprehensive bibliography of Schrödinger's scientific work, making it at the same time a book of acute contemporary relevance and a major work of reference. 252 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The Developing Brain: The Internal Environment of the Developing Brain; K.M. Dziegielewska, N.R. Saunders. Neuronal Migration and Differentiation During Normal and Genetically-Perturbed Development of the Hippocampal Formation; R.S. Nowakowski. Neurochemical Aspects of the Developing Brain: Dopaminergic Innervation of the Cerebral Cortex; B. Berger. Neuropathological Indicants of Neurodevelopmental Anomalies in Schizophrenics: Clinical and Neurodevelopmental Aspects of Brain Pathology in Schizophrenia; B. Bogerts, P. Falkai. Obstetrical Events and Adult Schizophrenia; K.L. Dykes et al. Structural Imaging and Neuropsychological Evidence of Neurodevelopmental Anomalies: Structural Brain Measurements on MRI Scans in Schizophrenia; H.A. Nasrallah, et al. Morphologic Markers of Neurodevelopmental Paths to Schizophrenia; R.M. Bilder, G.Degreef. Facts About Schizophrenia and Neurodevelopment: Neurodevelopment and Chronological Curiosities of Schizophrenia; P.B. Jones, et al. Three additional articles. Index. 256 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Atomic Inferometry and Trapping: Quantum Theory of Particle Trapping by Oscillating Fields; R.J. Glauber. Quantum Measurements on Localized Particles; S. Stenholm. Quantum Measurements: PhaseSensitive BackAction on a Microscopic System; V. Bagini, et al. Cavity QED and Classical Antenna Theory; J.P. Dowling. Quantum Noise Reduction and Squeezing: Progress and Perspectives in Squeezing; E. Giacobino, et al. Time-Dependent Invariants and Nonclassical Light; V.I. Man'ko. Optical Propagation, Communication and Imaging: Direct Space Quantum Optics; I. Abram, E. Cohen. Photonic Band Structure; E. Yablonovitch. Photon Interference and Bell's Theorum: What is Two Photons J.G. Rarity, P.R. Tapster. Quantum Cryptography and Bell's Theorum; A.K. Ekert. Twentyfive additional articles. Index. 444 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Clusters and superclusters of galaxies are the largest objects in the Universe. They have been the subject of intense observational studies at a variety of wavelengths, from radio to X-ray which has provoked much theoretical debate and advanced our understanding of the recent evolution of the large-scale structure of the Universe. The current status of the subject is reviewed in this volume by active researchers who lectured at a NATO Advanced Study Institute held in Cambridge, England in July 1991. Much of the material is presented in a pedagogical manner and will appeal to scientists, astronomers and graduate students interested in extragalactic astronomy. 384 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The book examines the operation of International Monetary Fund and World Bank conditionality in six developing countries (Argentina, Chile, Costa Rica, Jamaica, Mexico and Tanzania) and examines its effects on their economies. It draws conclusions and policy lessons for all developing countries as regards the operation of adjustment policies. The book also examines the regulatory treatment of Third World debt, both in the US, Canada and Europe, making specific policy suggestions for increasing flexibility in debt management. 347 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Taking into account recent developments in historical and ecological criticism, and incorporating fresh research into poetry and politics in the 1790s, the second edition of The Politics of Nature enlarges and updates Nicholas Roe's acclaimed study of Romanticism. Hitherto marginal figures are restored to prominence, and there is new material on William Wordsworth's radical years. The book includes the full text of John Thelwall's Essay on Animal Vitality with commentary, exploring how ideas of nature, revolution and radical science entwined. 186 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The essays in this collection fall into three groups. The first group deals with philosophical accounts of interpretation. The second is concerned with the interpretation of scripture with particular reference to the work of the Oxford theologian and philosopher Austin Farrer. The third group provides some examples of interpretative practice relating to Genesis and the book of Psalms. The contributors represent a wide range of academic disciplines and religious traditions, providing significant pointers for further developments in Biblical criticism and interpretation theory. 199 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The current debate about industrial relations cannot be understood without a knowledge of trade-union history. Dr Pelling's book, which has for several years been a standard work on the subject, has again been revised and updated to take account of recent research and to explain the course of events up to the Thatcher years, the miner's strike and the Employment Acts. The growth of white-collar unionism and the extension of women's rights are dealt with in the concluding chapters. 351 pp. Englisch.…

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume is dedicated to the memory of the late Charles J. Pedersen in recognition of his outstanding contribution to scientific research, culminating in his discovery of crown ethers and their remarkable cation complexing properties and his receipt of the 1987 Nobel Prize for Chemistry. The field of macrocyclic chemistry, to a large degree, had its beginnings with Professor Pedersen's discovery. A first-person account of this discovery is given as the first paper in this volume. The growth of the field since the first publication on the subject in 1967 has been enormous. Charles Pedersen lived to see much of this growth. He saw many of his own predictions of possible uses of crown ethers and related macrocycles realized. His work illustrates how one individual can make an enormous difference in science. The effect of his life and work on those who contributed papers for this volume and on many others is appreciated and is acknowledged by several of the authors in their individual papers. 416 pp. Englisch.…