springer new york springer us nov 2015 (36 risultati)

Taschenbuch. Condizione: Neu. Neuware -Almost every practical astronomer eventually aspires to have a fixed, permanent observatory for his or her telescope. A roll-off roof or dome observatory is the answer for the most popular home observatory design. Building a Roll-Off or Dome Observatory will help you decide whether to embark on the venture and will certainly increase your enthusiasm for the project.The author, both an amateur astronomer and a professional landscape architect, answers many of the common questions asked about observatory construction, covering the following topics:¿ Zoning, and by-law requirements common to most states, towns and municipalities¿ Where to locate the observatory¿ How to tailor the observatory for your particular needs¿ Tools and structural components required¿ Possible variations in design¿ How to combine the structure with other structures (incorporating a garden patio under the gantry inthe roll-off roof observatory, for example)This fully detailed outlines step-by-step construction, with professional detailed diagrams for each phase of construction.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 272 pp. Englisch.

Buch. Condizione: Neu. Neuware -This book explores five fundamental mechanisms to build secure Wireless Sensor Networks (WSNs). It presents security issues related to a single node which deals with the authentication and communication confidentiality with other nodes. It also focuses on network security, providing solutions for the node capture attack and the clone attack.It examines a number of areas and problems to which WSNs are applied continuously, including: supporting rescue operations, building surveillance, fire prevention, battlefield monitoring and more. However, known and unknown threats still affect WSNs and in many applications of this new technology the security of the network is a fundamental issue for confidentiality, integrity, authenticity and availability. The last section of the book addresses security for a common WSN service. Case studies are provided throughout.Secure Wireless Sensor Networks: Threats and Solutions targets advanced-level students and researchers in computer science and electrical engineering as a secondary text book. Professionals working in the wireless sensor networks field will also find this book useful as a reference.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 184 pp. Englisch.

Buch. Condizione: Neu. Neuware -This bookprovides a comprehensive overview of the operating principles andtechnology of electron lenses in supercolliders. Electron lenses are a novel instrument for high energy particle accelerators, particularly for the energy-frontiersuperconducting hadron colliders, including the Tevatron, RHIC, LHC and future very large hadron colliders. After reviewing the issues surrounding beam dynamics in supercolliders, the book offers an introduction to the electron lens method and its application. Further chapters describethe technology behindthe electron lenses which have recently been proposed, built and employed for compensation of beam-beam effects and for collimation of high-energy high-intensity beams, for compensation of space-charge effects and several other applications in accelerators.The book will be an invaluable resource for those involved in thedesign, construction andoperation of the next generation of hadron colliders.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 204 pp. Englisch.

Taschenbuch. Condizione: Neu. Neuware -This book offers a new framework for providing psychological services in schools at the individual, group, and systemic levels. It examines a variety of disorders common to school children, including anxiety, depression, ADHD, and conduct disorder, and outlines treatment options from evidence-based cognitive and cognitive-behavioral methods. The accessible real-world guidelines enable readers to design, implement, and evaluate interventions relevant to diverse student needs. Ethical, competency, and training concerns facing school practitioners in the new therapeutic environment are reviewed as well.Featured areas of coverage include:Behavioral assessment in school settings.PTSD and secondary trauma in children and adolescents.Transdiagnostic behavioral therapy for anxiety and depression in school.CBT for children with autism spectrum and other developmental disorders.Implementation, technological, and professional issues.The Practitioner's Toolkit: evidence-based cognitive and behavioral interventions.Cognitive and Behavioral Interventions in the Schools is an essential resource for professionals and scientist-practitioners in child and school psychology, social work, behavioral therapy, psychotherapy and counseling, and educational psychology.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 360 pp. Englisch.

Buch. Condizione: Neu. Neuware -This unique volume salutes the work of pioneering forensic psychologist Lawrence S. Wrightsman, Jr., by presenting current theorizing and research findings on issues that define the field of psychology and law. Ongoing topics in witness behaviors, suspect identification, and juror decision making illustrate how psychology and law complement and also conflict at various stages in legal processes. The book also sheds light on evolving areas such as DNA exonerations, professional trial consulting, and jury selection strategies, and the distinct challenges and opportunities these issues present. Noted contributors to the book include Wrightsman himself, who offers salient observations on the field that he continues to inspire.Featured among the topics:The credibility of witnesses.Psychological science on eyewitness identification and the U.S. Supreme Court.False confessions, from colonial Salem to today.Identifying juror bias: toward a new generation of jury selection research.Law and social science: how interdisciplinary is interdisciplinary enough Race and its place in the American legal system.With its diverse mix of perspectives and methodologies, The Witness Stand and Lawrence S. Wrightsman, Jr. will interest forensic researchers in academic and applied settings, as well as individuals working in the legal system, such as attorneys, judges and law enforcement personnel.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 184 pp. Englisch.

Taschenbuch. Condizione: Neu. Neuware -This brief systematically examines the trackback problem and its interaction with low-latency anonymous communication. First, it provides an overview of the common techniques a network-based attack may use to hide its origin and identity. Then the authors explore the MIX-based anonymity and the building blocks of low-latency anonymous communication. Later chapters offer a comprehensive study of the timing attacks on low-latency anonymous communication, and analyze the fundamental limitations of low-latency anonymous communication from the perspective of timing-based covert channel. Suitable for professionals and researchers, Traceback and Anonymity is a close look at a key aspect of cyber security studies. Advanced-level students interested in cyber security techniques or networking will also find the content valuable.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 88 pp. Englisch.

Buch. Condizione: Neu. Neuware -This book presents a new economic theory developed from physical and biological principles. It explains how technology, social systems and economic values are intimately related to resources. Many people have recognized that mainstream (neoclassical) economic theories are not consistent with physical laws and often not consistent with empirical patterns, but most feel that economic activities are too complex to be described by a simple and coherent mathematical theory. While social systems are indeed complex, all life systems, including social systems, satisfy two principles. First, all systems need to extract resources from the external environment to compensate for their consumption. Second, for a system to be viable, the amount of resource extraction has to be no less than the level of consumption. From these two principles, we derive a quantitative theory of major factors in economic activities, such as fixed cost, variable cost, discount rate, uncertainty and duration. The mathematical theory enables us to systematically measure the effectiveness of different policies and institutional structures at varying levels of resource abundance and cost.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 152 pp. Englisch.

Buch. Condizione: Neu. Neuware -This book provides a clear and accessible introduction to the essential mathematical foundations of linear canonical transforms from a signals and systems perspective. Substantial attention is devoted to how these transforms relate to optical systems and wave propagation. There is extensive coverage of sampling theory and fast algorithms for numerically approximating the family of transforms. Chapters on topics ranging from digital holography to speckle metrology provide a window on the wide range of applications.This volume will serve as a reference for researchers in the fields of image and signal processing, wave propagation, optical information processing and holography, optical system design and modeling, and quantum optics. It will be of use to graduate students in physics and engineering, as well as for scientists in other areas seeking to learn more about this important yet relatively unfamiliar class of integral transformations.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 472 pp. Englisch.

Buch. Condizione: Neu. Neuware -This volume provides updates of this established field in both methods and applications, as well as advances in applications of the microarray method to biomarkers such as DNAs, RNAs, proteins, glycans and whole cells. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Microarray Technology: Methods and Applications aims to ensure successful results in the further study of this vital field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 308 pp. Englisch.

Buch. Condizione: Neu. Neuware -This third edition is comprised of well-established protocols that are considered the gold standard in the field as well as new methodologies for mitochondrial DNA analysis. of Mitochondrial DNA: Methods and Protocols describes protocols for detecting mutations in mitochondrial DNA, techniques to assess mitochondrial DNA damage, visualization of mitochondrial DNA in situ, detection of mitochondrial DNA nucleoids within the mitochondria, methods for analyzing mitochondrial DNA replication, mitochondrial DNA-encoded protein translation and mitochondrial DNA copy number, the latest technologies for modifying the mitochondrial genome and methods for the purification of proteins involved in the replication and transcription of mitochondrial DNA. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Mitochondrial DNA: Methods and Protocols, Third Edition will be useful not only for mitochondrial researchers but also for scientists studying human diseases where mitochondrial DNA variation has been recognized as an important pathogenic factor, including cancer and neurodegeneration.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 252 pp. Englisch.

Buch. Condizione: Neu. Neuware -This volume highlights recent developments in flow cytometry, affinity assays, imaging, mass spectrometry, microfluidics and other technologies that enable analysis of proteins at the single cell level. The book also includes chapters covering a suite of biochemical and biophysical methods capable of making an entire gamut of proteomic measurements, including analysis of protein abundance or expression, protein interaction networks, post-translational modifications, translocation and enzymatic activity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Authoritative and thorough, Single Cell Protein Analysis: Methods and Protocols is useful to researchers and students in biological and biomedical sciences who have an interest in proteomic measurements in cells.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 268 pp. Englisch.

Buch. Condizione: Neu. Neuware -This second edition provides up-to-date protocols focusing on techniques for the specific enrichment of phosphopeptides and phosphoproteins. Phosphoproteomics Methods and Protocols, Second Edition guides readers through different labeling strategies for quantitative phosphoproteomics; high-throughput mass spectrometry-based phosphoproteome analyses and phospho flow cytometry; and bioinformatics strategies for phosphoproteomics data analysis and integration. Additional protocols concentrate on the identification of kinase-substrate relationships by both high- and low-throughput approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Phosphoproteomics Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 380 pp. Englisch.

Buch. Condizione: Neu. Neuware -This valuable collection aims to provide a collection of frequently used statistical methods in the field of proteomics. Although there is a large overlap between statistical methods for the different ¿omics¿ fields, methods for analyzing data from proteomics experiments need their own specific adaptations. To satisfy that need, Statistical Analysis in Proteomics focuses on the planning of proteomics experiments, the preprocessing and analysis of the data, the integration of proteomics data with other high-throughput data, as well as some special topics. Written for the highly successful Methods in Molecular Biology series, the chapters contain the kind of detail and expert implementation advice that makes for a smooth transition to the laboratory.Practical and authoritative, Statistical Analysis in Proteomics serves as an ideal reference for statisticians involved in the planning and analysis of proteomics experiments, beginners as well as advanced researchers, and also for biologists, biochemists, and medical researchers who want to learn more about the statistical opportunities in the analysis of proteomics data.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 324 pp. Englisch.

Buch. Condizione: Neu. Neuware -This atlas provides a comprehensive, state-of-the-art review of all interventions that pertain to the esophagus. It includes a review of the current staging modalities, ablation technologies, resection and reconstruction techniques, and disease classification. Evidence-based guidelines regarding how each intervention is chosen are also included. With color illustrations and photographs for each surgery, the atlas details specific anatomic topics such as micro-anatomy of Barrett¿s and Dysplasia, EMR pathology, endoscopic ultrasound, and conventional surgical anatomy. Each intervention is presented in task format as a task list to be checked-off as each step is completed.Written by experts in the field, Atlas of Esophageal Disease and Intervention: A Multidisciplinary Approachserves as a valuable resource for any practitioner who performs esophageal intervention and will guide new surgeons and gastroenterologists into the hybrid multidisciplinary approach to this disease.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 248 pp. Englisch.

Buch. Condizione: Neu. Neuware -This volume provides readers with an introduction to all the methods and major approaches now being used to study the structural and functional development of the brain. Prenatal and Postnatal Determinants of Development explores early anatomical development, the emergence of function and the processes that lead to damage and repair of the growing and immature brain.Chapters are divided into three sections covering development, programming and stress, and brain damage-causes and consequences. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.Concise and easy-to-use, Prenatal and Postnatal Determinants of Development aims to ensure successful results in the further study of this vital field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 380 pp. Englisch.

Buch. Condizione: Neu. Neuware -This volume presents protocols on yeast cytokinesis that will help Molecular and Cellular Biology researchers in the use of these microorganisms to approach the study of general or specific key questions in cytokinesis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Yeast Cytokinesis: Methods and Protocols provides practical and step-by-step detailed protocols useful for a wide audience ranging from experienced researchers to beginners in the use of yeasts.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 408 pp. Englisch.

Buch. Condizione: Neu. Neuware -This volume provides detailed discussion of a variety of important techniques that researchers use to study fungal molecular biology and pathogenesis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Candida Species: Methods and Protocols aims to ensure successful results in the further study of this vital field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 252 pp. Englisch.

Buch. Condizione: Neu. Neuware -This second volume expands upon the previous edition with new research and objectives in immunoregulation and immune tolerance. Chapters cover topics ranging from new molecular and cellular mechanisms of tolerance; generation and characterization of mice regulatory macrophages; recent advances in the treatment of immune-mediated inflammatory disorders; and novel mechanisms and therapeutic perspectives on food allergies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Suppression and Regulation of Immune Responses: Methods and Protocols, Volume II is a great resource for current research and inspiration for new studies in immune tolerance.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 276 pp. Englisch.

Buch. Condizione: Neu. Neuware -This volume provides up-to-date scientific achievements from the world¿s top researchers. Recombinant Proteins from Plants: Methods and Protocols, Second Edition guides readers through protocolsfor use with a variety of plant expression systems. Various aspects of production are covered including vector selection and cloning; product improvements for stability, glycosylation, and antibiotic-free selection; extraction and scale-up; and analysis of transgenic plants and their recombinant proteins. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Recombinant Proteins from Plants: Methods and Protocols, Second Edition is an ideal reference for those who are interested in plant molecular biology and molecular farming.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 312 pp. Englisch.

Buch. Condizione: Neu. Neuware -This volume details methods and protocols for the synthesis and characterization of glycopolymers and their biomedical applications. Chapters are divided into three parts covering synthesis and characterization of glycopolymers; glycopolymer-nanoparticle conjugates; and surface immobilized glycopolymers. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Macro-Glycoligands: Methods and Protocols aims to ensure successful results in the further study of this vital field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 240 pp. Englisch.

Buch. Condizione: Neu. Neuware -As there is a high incidence of disease recurrence after liver transplantation, this volume is designed around the need for a reference text dealing exclusively with this problem. The book places special emphasis on pre- and post-transplant predictors of recurrence, severity assessment, prophylaxis, and treatment. Pathobiology of disease recurrence is discussed in detail where applicable. Issues including quality of life and cost burden are also covered in the text.Written by well-known authorities in each field, Disease Recurrence After Liver Transplantation: Natural History, Treatmentand Survival serves as a comprehensive reference for physicians and surgeons who take care of liver transplant recipients and a major addition to the literature detailing the current understanding of disease recurrence.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 284 pp. Englisch.

Buch. Condizione: Neu. Neuware -The type 2 immune response that develops during infectious disease has undergone major paradigm shifts in the last several years as new cell types and pathways have been identified. It is now clear that the type 2 immune response, characterized by elevations in specific cytokines, including IL-4, IL-5 and IL-13, is associated with helminth infections in both humans and mice.This response is complex and includes effector functions that mediate resistance, contributing to expulsion and in some cases destruction, of the parasite.But just as importantly, the type 2 immune response can also mediate tolerance mechanisms, which can mitigate tissue injury as these large multicellular parasites transit through vital organs.The tolerance mechanisms include both tissue repair and immune regulatory effects. These latter aspects of the helminth-induced type 2 immune response are increasingly recognized as a potential resource that can be mined for the development of novel immunotherapies that may enhance wound healing, control of autoimmune and inflammatory diseases and regulation of metabolic homeostasis.In this book, leading researchers in this exciting and dynamic field discuss the latest findings and emerging concepts, providing an intellectual framework that can be used as a basis for new discoveries and potentially new treatments for diseases associated with inflammation.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 188 pp. Englisch.

Taschenbuch. Condizione: Neu. Neuware -This book covers issues of men¿s health, including screening, diagnosis and management of common disorders and opportunities for prevention and health maintenance. Each chapter pertains to a component of the physical exam and/or organ system. Examples include appropriate survey and screening of the integument, the significance of buccal/dental integrity, tailoring cardiovascular remedies and balancing prostate cancer screening with quality of life. The book follows the methodical approach of a comprehensive wellness visit, including inventory of psychosocial factors, which significantly impact physical well-being. Justification for often overlooked topics in the review of systems, such as sexual history and satisfaction, relationship issues, and vocational fulfillment are provided. This book is written in the spirit of the rich bedside acumen and the art of healing, we must strive to protect. While informative and stimulating, this text serves to remind us of the specialized diagnostic tools we carry between our ears, and the power of healing we possess in our hands as well as the counsel and education we provide to our patients.Men¿s Health: A Head to Toe Guide for Cliniciansserves as an important and readily accessible resource for primary care physicians, internists and family doctors, physician¿s assistants and nurse practitioners who care for adult males.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 260 pp. Englisch.

Buch. Condizione: Neu. Neuware -This fully updated volume reflects the spectacular advances in our knowledge of signal transduction pathways with a selection of ¿classic¿ as well as newly developed approaches. These detailed approaches expand into the fields of molecular biology, biochemistry, physiology, cell biology, genetics, and genomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Practical and up-to-date, Plant Signal Transduction: Methods and Protocols, Second Edition serves as an ideal guide for researchers exploring the vast array of signals produced by plants to ensure their survival.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 260 pp. Englisch.

Buch. Condizione: Neu. Neuware -This work provides in-depth analysis of the origins of landscape ecology and its close alignment with the understanding of scale, the causes of landscape pattern, and the interactions of spatial pattern with a variety of ecological processes. The text covers the quantitative approaches that are applied widely in landscape studies, with emphasis on their appropriate use and interpretation.The field of landscape ecology has grown rapidly during this period, its concepts and methods have matured, and the published literature has increased exponentially. Landscape research has enhanced understanding of the causes and consequences of spatial heterogeneity and how these vary with scale, and they have influenced the management of natural and human-dominated landscapes. Landscape ecology is now considered mainstream, and the approaches are widely used in many branches of ecology and are applied not only in terrestrial settings but also in aquatic and marine systems. In response to these rapid developments, an updated edition of Landscape Ecology in Theory and Practice provides a synthetic overview of landscape ecology, including its development, the methods and techniques that are employed, the major questions addressed, and the insights that have been gained.¿Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 504 pp. Englisch.

Buch. Condizione: Neu. Neuware -This exposition provides the state-of-the art on the differential geometry of hypersurfaces in real, complex, and quaternionic space forms. Special emphasis is placed on isoparametric and Dupin hypersurfaces in real space forms as well as Hopf hypersurfaces in complex space forms. The book is accessible to a reader who has completed a one-year graduate course in differential geometry. The text, including open problems and an extensive list of references, is an excellent resource for researchers in this area.Geometry of Hypersurfaces begins with the basic theory of submanifolds in real space forms. Topics include shape operators, principal curvatures and foliations, tubes and parallel hypersurfaces, curvature spheres and focal submanifolds. The focus then turns to the theory of isoparametric hypersurfaces in spheres. Important examples and classification results are given, including the construction of isoparametric hypersurfaces based on representations of Clifford algebras. An in-depth treatment of Dupin hypersurfaces follows with results that are proved in the context of Lie sphere geometry as well as those that are obtained using standard methods of submanifold theory. Next comes a thorough treatment of the theory of real hypersurfaces in complex space forms. A central focus is a complete proof of the classification of Hopf hypersurfaces with constant principal curvatures due to Kimura and Berndt. The book concludes with the basic theory of real hypersurfaces in quaternionic space forms, including statements of the major classification results and directions for further research.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 608 pp. Englisch.

Buch. Condizione: Neu. Neuware -This volume captures the rapid developments in the field of induced pluripotent stem (iPS) cells, which have provided novel opportunities and approaches both for better understanding a number of human diseases and for developing new platforms for drug development and screening for such diseases. Specifically, representative protocols on various disease models have been collected from labs around the world. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Patient-Specific Induced Pluripotent Stem Cell Models: Generation and Characterization is an ideal reference for scientists working on furthering iPS research.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 384 pp. Englisch.

Buch. Condizione: Neu. Neuware -This volume examines a wide array of vital technologies for advancing our understanding of the receptor-mediated actions of estrogen. The protocols in this book range from standard methods and vital laboratory workhorses, such as receptor binding assays and western blot, to newer technologies such as RNAseq and proximity ligation assay. Chapters also discuss protocols from a broad range of tissue types to demonstrate the variety of estrogen receptor effects. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Estrogen Receptors: Methods and Protocols is a detailed and helpful resource for scientists who are intrigued by the many facets of estrogen.The chapter 'Bioinformatics Analysisof Estrogen-Responsive Genes' is open access under a CC BY 4.0 license.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 600 pp. Englisch.

Buch. Condizione: Neu. Neuware -This book presents cutting edge methods that provide insights into the pathways by which salt and water traverse cell membranes and flow in an orchestrated fashion amongst the many compartments of the body. It focuses on a number of molecular, cellular and whole animal studies that involve multiple physiological systems and shows how the internal milieu is regulated by multifactorial gene regulation, molecular signaling, and cell and organ architecture.Topics covered include: water channels, the urinary concentrating mechanism, angiotensin, the endothelin system, miRNAs and MicroRNA in osmoregulation, desert-adapted mammals, the giraffe kidney, mosquito Malpighian tubules, and circadian rhythms. The book highlights how different approaches to explaining the same physiological processes greatly increase our understanding of these fundamental processes. Greater integration of comparative, evolutionary and genetic animal models in basic science and medical science will improve our overall grasp of the mechanisms of sodium and water balance.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 332 pp. Englisch.

Buch. Condizione: Neu. Neuware -This volume details up-to-date information and attempts to take the reader into the exciting realm of TGF-¿ from the basic principles to the practical applications. Chapters provide basic introduction of TGF-¿ signaling from the cell surface to the nucleus, methods and techniques for the investigation of TGF-¿ signaling mechanism including receptors, Smads, intracellular kinases, microRNA, epigenetic regulation, post-translational regulations, non-Smad pathway; the physiological implications including those in cell cycle arrest, epithelial-mesenchymal transition, endothelial cells, adipogenesis, Th differentiation, stem cell, bone remodeling, ovary, zebrofish development, and frog animal capping; and the methodologies including metastasis imaging, 3D morphogenesis, membrane receptor quantification, conditional knockout, bone remodeling, kinase and phosphatase assays, BiFC interaction assays, and genome-wide siRNA screen. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, TGF-Beta Signaling: Methods and Protocols aims to ensure successful results in the further study of this vital field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 408 pp. Englisch.