springer us aug 2021 (50 risultati)

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume details protocols encompassing different aspects of computer aided design and manufacturing of 3D scaffolds and biofabricated constructs for tissue engineering applications. Chapters are divided into four parts covering optimization of scaffold architectures for computer aided tissue engineering, synthetic routes to biomaterials compatible, technological platforms and manufacturing processes, and relevant applicative scenarios. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Computer-Aided Tissue Engineering: Methods and Protocols aims to be useful for new and experienced laboratory researchers working on different aspects of corneal regeneration. 200 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume gives a comprehensive insight into established and novel methods to analyze the structure and function of lipid rafts. This book covers topics such as isolation of lipid rafts and their functional analysis using biochemical methods; visualization of lipid rafts and their interaction with proteins using fluorescence-related methods; preparation of giant lipid vesicles and fluorescence spectroscopy; FRET and FRAP; and using photo-activated cross-linking of a ceramide analog combined with proximity ligation assay. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Thorough and cutting-edge, Lipid Rafts: Methods and Protocols is a valuable resource for both novice and expert researchers interested in learning more about the function of lipid rafts in many areas of cell biology and medicine. 364 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book illustrates experimental and computational methodologies used to achieve cost effective biological processes for the production of fuels and biochemicals through multiple approaches to increasing yield, titers, and productivity in a robust host. The volume includes the most recent and cutting-edge aspects of pathway engineering, flux analysis, and metabolic enzyme engineering. Each chapter highlights the complexity and challenges of the problem as well as the methods used to solve this problem or changes needed in current methods. As a part of the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that gives researchers a much needed boost.Authoritative and practical, Metabolic Pathway Engineering benefits not only scientists working on more fundamental aspects of this endeavor but also those in the biochemical industry working on strain engineering for robust industrial processes. 248 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume includes in-depth, hands-on protocols for detecting various developmental defects in Drosophila. It provides cutting-edge methods for maintaining Drosophila under laboratory conditions to perform various experiments, and for dissecting various imaginal discs of the larvae. Further, biochemical protocols for estimating the levels of different metabolites, reactive oxygen species, and fat-sensitive pathways are discussed, and various staining and behavior techniques for fat detection are provided. The book explains how various fluorescent dyes and the comet assay can be used to identify DNA damage, and elaborates on the analysis of the eye, antennae, imaginal disc, gut and muscle damage under bright field and fluorescent microscopes. It covers the analysis of hemolymph using Giemsa staining; determining the functionality of channel proteins, eye and mechanosensory organs in adults and larvae using various behavioral assays; and metal detection and structural analysis for various tissues using SEM. In closing, it addresses the analysis of Drosophila's eye and head via paraffin section; measurement of reactive oxygen species from various tissues via FACS; and the CRISPER technique for gene editing and analysis of micro RNA mutations. 276 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores the latest metabolic flux analysis (MFA) techniques that cover the analysis of cellular, organ level, and whole-body metabolism. The chapters in this book discuss topics such as deutrium tracing; isotopologue fractions using GC-TOF; non-targeted mass isotopolome analysis; large-scale profiling of cellular metabolic activities using deep 13C labeling medium; metastases in mice; SWATH; Exo-MFA; metabolic flux from time-course metabolomics; and thermodynamic approaches in flux analysis. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive,Metabolic Flux Analysis in Eukaryotic Cells: Methods and Protocolsis a valuable resource for both experts in MFA techniques and researchers getting involved in the role of quantitative studies to uncover the dysregulated pathways in human diseases. 384 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This updated book covers a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches. It includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, organelle RNAs, endosymbiont RNAs, and alternatively spliced RNA variants from various organisms. RNA editing and the computational data processing for genome-wide datasets is also discussed. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, RNA Abundance Analysis: Methods and Protocols, Second Edition provides a comprehensive set of techniques and methods on isolating and analyzing mRNAs, small RNAs, and other RNA variants, in order to assist researchers in their gene expression studies. 248 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume presents the latest techniques used to study germ cell tumors (GCTs), including histopathology; in vitro and in vivo techniques for investigating GCT biology; novel methods for detecting minimal residual diseases; and sequencing considerations as they apply to GCTs. Chapters cover topics such as clinical applications of immunohistochemistry in GCTs in men; fluorescence in situ hybridization (FISH) detection of chromosomal 12p anomalies in testicular GCTs; evaluation of chemotherapeutic drugs for treatment of (cisplatin-resistant) germ cell cancer cell lines; targeted methylation analyses; and circulating microRNA panel for malignant GCT diagnosis and monitoring. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Testicular Germ Cell Tumors: Methods and Protocols provides valuable information that is useful for both novice and expert GCT researchers interested in learning more about this field. 292 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume looks at major clinical trials for motor and non-motor symptoms in Parkinson's Disease (PD) and covers important aspects, including trial design, sample selection, and outcome selection. Chapters in this book discuss topics such as toxin-based rodent or genetic models of PD; clinical trials for motor symptoms, L-DOPA related motor complications, and gait disorders; clinical trials for mood disorders, troubled sleep, autonomic dysfunction; and clinical trials for disease modifying therapies. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory or research center.Cutting-edge and authoritative, Clinical Trials in Parkinson's Disease is a valuable resource for clinicians and researchers who want to enhance their interpretation of results from clinical trials and to design their own high-quality trials. 412 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed book explores a diverse range of topics related to wound healing. Some areas include wound generation as a tool to study cellular interactions and growth, mechanisms by which different tissues regenerate, ischemic wounds and their treatment, repairing corneal wound, as well as a number of emerging technologies to help promote wound regeneration, such as techniques with miRNA, nanomaterials, biomaterials, and stem cell therapies. Written for the highly successful Methods in Molecular Biology series, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Wound Regeneration: Methods and Protocols serves as a vital collection of wound model development and regeneration studies that will be of great use in both the academic and industrial fields of research. 220 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed book showcases the tremendous effort and progress made in developing techniques and protocols for the study of immunometabolism, and in utilizing recent technological advances for probing and manipulating adipose and immune cells, and subsequently, their functions and immunometabolic consequences. Written by experts in the field, many chapters use macrophages as a model immune cell type, due to their prominence in the innate immune system and the exhaustive study of their traits. Protocols using adipocytes, dendritic cells, and T cells as model cell lines, as well as measurement of glucose metabolism at the systemic level, have also been included. Written for the highly successful Methods in Molecular Biology book series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Immunometabolism: Methods and Protocols serves as a vital guide for researchers working at the important interface of immunology and metabolism. 300 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides a comprehensive collection of DNA assembly protocols that will prove useful for any researcher interested in molecular cloning, synthetic biology, or DNA manipulation. Chapters will guide readers through computational tools to design and track the construction of DNA assemblies, workflows that enable high-throughput assembly of DNA constructs, standardized toolkits and protocols for DNA assembly, and combinatorial solutions that enable the construction and optimization of entire metabolic pathways. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, DNA Cloning and Assembly: Methods and Protocols aims to ensure successful results in the further study of this vital field. 348 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition volume expands on the previous edition with a look at the latest techniques in plant epigenetics and epigenomic research. Chapters in this book cover topics such as whole genome methylome analysis; analysis of DNA methylation; chromatin analysis of metabolic gene clusters in plants; plant epigenetic stress memory induced by drought; small non-coding RNA analysis in seeds of forest tree species; and a discussion on epigenetics inheritance in plants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Plant Epigenetics and Epigenomics: Methods and Protocols, Second Edition is a valuable resource for both novice and expert researchers, and aims to advance this field of study by paving the way for integrating epigenetic mechanisms into models of plant function during development and evolution. 284 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides a varied selection of current methods and molecular probes used to generate, image and quantify ROS production and lipid peroxidation. Chapters guide readers through methods and protocols on in silico modelling of ROS dynamics in biological systems and a breaking proposal. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Reactive Oxygen Species: Methods and Protocols aims to ensure successful results in the further study of this vital field. 248 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed new edition provides a comprehensive collection of protocols applicable to all members of the Coronavirinae sub-family currently and that are also transferrable to other fields of virology. Beginning with a section on detection, discovery, and evolution, the volume continues with coverage of propagation and titration of coronaviruses, genome manipulation, study of virus-host interactions, as well as imaging coronavirus infections. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Coronaviruses: Methods and Protocols, Second Edition serves as a valuable guide to researchers working to identify and control viruses with increased potential to cross the species barrier and to develop the diagnostics, vaccines, and antiviral therapeutics that are required to manage future outbreaks in both humans and animals. 292 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume details practical procedures on the latest DNA vaccine technology. Chapters guide readers through methods and protocols on DNA vaccine design, the adjuvant influence, production and purification methodologies, delivery systems, and approaches of the influence of DNA vaccines in the immunological response performance and in the cancer immunotherapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,DNA Vaccines: Methods and Protocols aims to ensure successful results in the further study of this vital field. 348 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book details the synthesis and assembly of polypeptide materials across length scales, i.e. proteins and peptides, their precursors, conjugates, and derivatives. A particular emphasis is made on measurement tools and procedures for material characterization, both physicochemical and functional. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical,Polypeptide Materials: Methods and Protocols serves to reflect the inter-disciplinary nature of molecular biology as well as the importance of developing innovative measurement methods to advance this vital area of research. 284 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book addresses the major periodontal pathogens implicated as causal agents in periodontal disease, including Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans, and Prevotella spp. Beginning with methods for bacterial genetic manipulation, the volume continues with sections on experimental methods to examine virulence factors, interactions with other pathogenic microorganism and host cells, as well as a chapter on an animal model of periodontitis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Periodontal Pathogens: Methods and Protocols serves as an extensive and useful reference for researchers studying periodontal pathogens and will help elucidate the causes of periodontal disease and the systemic diseases related to it. 268 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume looks at the latest research techniques to study the interaction of visual spatial learning and attention guidance with behavioral, psychophysiological, and imaging methods. Part One (behavioral methods) focuses on different paradigms of visual search like visual foraging and contextual cueing, and also methods like feature distribution analysis and search in virtual reality. Part Two (psychophysiological methods) integrates innovative uses of classical potential changes like the CDA and N2pc, with multivariate analysis methods and multi-method designs. Part Three (functional imaging) covers lesion-behavior mapping, retinotopic and grid cell mapping methods for human fMRI, as well as functional registration by hyperalignment and simultaneous eye-tracking and fMRI. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Cutting-edge and comprehensive, Spatial Learning and Attention Guidance is a valuable resource for all researchers and scientists who are interested in learning more about the relationship between attention and memory. 328 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume presents examples of how Artificial Neural Networks (ANNs) are applied in biological sciences and related areas. Chapters cover a wide variety of topics, including the analysis of intracellular sorting information,prediction of the behavior of bacterial communities, biometric authentication, studies of Tuberculosis, gene signatures in breast cancer classification, the use of mass spectrometry in metabolite identification,visual navigation, andcomputer diagnosis. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical,Artificial Neural Networks: Third Edition should be of value to all scientists interested in the hands-on application of ANNs in the biosciences. 372 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This fully updated edition provides the most recent advances in cancer cell signaling knowledge combined with some discussion of the current challenges and prospects in cancer therapy. Beginning with an important section on cancer resistance, the main obstacle to effective cancer therapy, the book continues with chapters exploring state-of-the-art methods in epigenetic control of cancer, metastasis promotion, as well as a variety of new technologies in the study of cancer. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and timely, Cancer Cell Signaling: Methods and Protocols, Third Edition serves as an ideal guide to researchers seeking to overcome the challenges in the vital field of cancer cell signaling. 312 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book presents key methodologies, tools and databases for biochemistry, microbiology and molecular biology in simple and straightforward language. Covering all aspects related to experimental principles and procedures, the protocols included here are brief and clearly defined, and include essential precautions to be taken while conducting experiments. The book is divided into two major sections: one on constructing, working with, and standard operating procedures for laboratory instruments; and one on practical procedures used in molecular biology, microbiology and biochemical analysis experiments, which are described in full.Each chapter describes both the basic theory and relevant practical details for a given experiment, and helps readers recognize both the experiment's potential and limitations. Intended as an intensive introduction to the various tools used in molecular biology, the book covers all basic methods and equipment, including cloning, PCR, spectrophotometers, ELISA readers, sonicators, etc. As such, it offers a valuable asset for final year undergraduate (especially project) students, graduate research students, research scientists and technicians who wish to understand and employ new techniques in the field of biotechnology. 296 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume discusses the oral microbiome, and oral and systemic health. The chapters in this book cover topics such as analytical techniques for identifying and measuring oral bacteria; strategies for controlling common sources of variability in oral microbiome methods for viral bacterial and fungal analysis; ways to study oral DNA and RNA samples to identify molecular pathways to disease; approaches to functional assays for oral bacteriophage, antibiotic purging of systemic bacteria; and metaproteomic analysis of various oral samples. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and practical, The Oral Microbiome: Methods and Protocols is a valuable resource for any scientist or researcher looking to further study this exciting and developing field. 312 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed book collects methods based on the evolution of the chromosome conformation capture (3C) technique and other complementary approaches to dissect chromatin conformation with an emphasis on dissection of nuclear compartmentalization and visualization in imaging. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Capturing Chromosome Conformation: Methods and Protocols serves as an ideal guide for researchers working to further understand 3D genome organization. 336 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition provides updated and new chapters to build on and extend the strengths of the first edition. Chapters guide readers through basic biology of basophils, obtaining the cells by purification, culture of stem cells progenitors, peripheral CD34+ stem cell-derived mast cells, basophils from CD34+ progenitors, diagnostic applications, gene expression patterns in basophils, roles of basophils in different asthma phenotypes, knockout, and disease models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Basophils and Mast Cells: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field. 400 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume contains a collection of innovative techniques for studying targeted protein degradation. Chapters guide readers through heterobifunctional proteolysis-targeting chimeras (PROTACs) approaches, E3 ligase, E3 ligase-induced ubiquitylation, proteomic approaches, novel degrader molecules, molecular glue, and stabilize binding interaction between a target and E3 ubiquitin ligase. Written in the format of the highly successfulMethods in Molecular Biologyseries, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.Authoritative and cutting-edge, Targeted Protein Degradation: Methods and Protocols aims to ensure successful results in this emerging field of drug discovery. 364 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores homologous recombination's (HR) essential role in meiotic and somatic cells. It discusses the analysis of different steps of the HR process from the genetic, molecular biology, and cell biology perspectives. Using a variety of model systems, chapters in this book cover topics such as the genome-wide mapping of DNA double-strand breaks (DSB); analysis of DNA-end resection and recombination intermediates by gel electrophoresis and southern blotting; cell-based monitoring of HR activity in meiotic and mitotic cells; in vitro analysis of branch migration, DNA strand exchange and Holliday junction resolution; or super resolution imaging of HR repair at collapsed replication forks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Homologous Recombination: Methods and Protocolsis a valuable resource that uses both classical and more recent approaches to answer questions on the HR mechanism. It is a useful tool for scientists working on the field of genome integrity, as well as those working in cancer biology and DNA repair. 576 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book provides an overview of methods and experimental protocols that are currently used to analyze the presence and abundance of non-canonical DNA nucleotides in different biological systems. Focusing particularly on the newly discovered and less studied DNA modifications that are enzymatically produced and are likely to play specific roles in various biological processes, the volume explores chromatography- and mass spectrometry-based techniques for the detection and quantification of DNA modifications, antibody-based approaches to study their spatial distribution in different cells and tissues, and methods to analyze their genomic distribution with the help of bioinformatics tools that interrogate the corresponding datasets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and comprehensive, DNA Modifications: Methods and Protocols serves as an ideal guide to research scientists and PhD students in this rapidly developing discipline, and, thus, will ultimately contribute to deciphering the roles of non-canonical DNA nucleotides in different biological systems. 512 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This book presents key methodologies, tools and databases for biochemistry, microbiology and molecular biology in simple and straightforward language. Covering all aspects related to experimental principles and procedures, the protocols included here are brief and clearly defined, and include essential precautions to be taken while conducting experiments. The book is divided into two major sections: one on constructing, working with, and standard operating procedures for laboratory instruments; and one on practical procedures used in molecular biology, microbiology and biochemical analysis experiments, which are described in full.Each chapter describes both the basic theory and relevant practical details for a given experiment, and helps readers recognize both the experiment¿s potential and limitations. Intended as an intensive introduction to the various tools used in molecular biology, the book covers all basic methods and equipment, including cloning, PCR, spectrophotometers, ELISA readers, sonicators, etc. As such, it offers a valuable asset for final year undergraduate (especially project) students, graduate research students, research scientists and technicians who wish to understand and employ new techniques in the field of biotechnology.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 296 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The volume aims to explore the dynamic nature of the autophagy pathway, and the latest techniques that allow researchers to capture and quantify this process in neurons. The chapters in this volume cover topics such as fundamental, historical, and functional approaches that began in baker's yeast; Saccharomyces cerevisiae; the role of both electron microscopy and live-cell imaging using fluorescently tagged autophagy proteins; and the rate of puncta appearance and its correlation with the rate of autophagosome formation. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Cutting-edge and practical, Imaging and Quantifying Neuronal is a valuable resource that provides insights into the power of microscopy tools, live-cell imaging, and photoactivation and correlative techniques. 164 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware 392 pp. Englisch.