springer us feb 2025 (30 risultati)

Taschenbuch. Condizione: Neu. Neuware -This collection explores the use of pluripotent cells to generate early embryo-like structures in vitro, with the resultant 3D embryo-like structures in vitro phenocopying many of the developmental landmarks and 3D architectures seen normally in vivo. The protocols gathered herein cover diverse aspects of the topic and exemplify some of the key developments and improvements in the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 336 pp. Englisch.

Buch. Condizione: Neu. Neuware -This fully updated volume presents protocols crucial for contemporary influenza virus research, placing a special emphasis on the latest advancements in imaging technologies, including super-resolution microscopy, cryo-electron tomography (cryo-ET), freeze replica electron microscopy, and high-speed atomic force microscopy (AFM). In addition to covering traditional methods like virus purification, this edition introduces innovative approaches for visualizing molecular interactions between viral proteins and host proteins, methods for developing physiological cell culture models and animal models, as well as concepts concerning the prediction of viral evolution and the utilization of artificial intelligence in infection biology research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 372 pp. Englisch.

Buch. Condizione: Neu. Neuware -This second edition volume expands on the previous edition with updates on the latest methodologies in the transcriptomics field. The chapters in this book cover topics such as spatial omics, long-read sequencing technology, tissue microarrays, analysis of saliva and extracellular vesicles, machine learning and artificial intelligence-based approaches for analysis of singe cells transcriptome, and large sets of data on multi-omics including transcriptomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and practical, Gene Expression Analysis: Methods and Protocols, Second Edition is a valuable resource for advanced undergraduate and graduate students studying gene expression analysis, and scientists interested in learning more about this rapidly advancing field.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 380 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed volume examines classical and cutting-edge methods involving double-stranded RNA (dsRNA), specifically regarding isolation, visualization, characterization, production, and application. Many protocols, such as co-immunoprecipitation-based isolation of double-stranded RNA-associated protein complexes, identification of mycoviruses by dsRNA extraction, application of dsRNA for fungi disease management (Sclerotinia sclerotiorum and Botrytis cin), and production of double-stranded RNA in plants by plant viral vectors for gene silencing, can also be easily adapted for identification of viruses from other organisms, control of other pathogens, and fundamental research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls.Reliable and practical, Double-Stranded RNA: Methods and Protocols serves as an ideal reference book for students and researchers who work with dsRNA. 142 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume discusses the latest advancements in modern methodologies used to study liver carcinogenesis. The first half of this book describes pertinent preclinical models of hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA), established either through orthotopic induction of ectopic implantation. The second half of this book covers a diverse array of techniques applied to characterize the biochemical and cellular composition of hepatic malignancies that operate at the single-cell and histological levels. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Liver Carcinogenesis: Methods and Protocols, is a valuable resource for students and scientists who are interested in drivingprogress in the important field of liver cancer research. 228 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores the latest ways to detect hypoxia in the context of cancer, including techniques to study gene expression changes, protein responses, and cellular adaptations to low-oxygen conditions. This book also covers new protocols to characterize hypoxia in tumors with high spatial resolution and novel therapeutic approaches that consider the complex microenvironments of solid tumors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Hypoxia: Methods and Protocols is a valuable tool to help cancer researchers and other scientists detect hypoxia in cancer and other disease pathologies. 280 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed volume explores techniques for the isolation, purification, and establishment of in vitro germ cell (GS) systems at different stages of development in a variety of organisms, including chickens, mice, rats, and humans. The book additionally describes cutting-edge analytic and informatic tools to study germ cell development at the single cell level and meiotic recombination. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Germ Cell Development: Methods and Protocols provides new insight into the understanding of basic biological aspects of germ cell biology, as well as the opportunity for in vitro manipulation of germ cells for toxicology studies and for genetic intervention. 300 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume presents vital techniques for the commercial micropropagation of plants. It explores numerous micropropagation protocols in different types of temporary immersion systems for plant species with agricultural, medicinal, ornamental, and forestry interest, detailing the type of bioreactor and the optimal parameters necessary to guarantee obtaining the largest number of commercial propagules. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Micropropagation Methods in Temporary Immersion Systems serves as an ideal guide to allow for the semi-automation and reduction of production costs during the micropropagation of plants. 252 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware 452 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores the latest advancements in mucin research. The chapters in this book are organized into 8 parts and cover a wide range of topics such as mucin extraction, isolation, physicochemical property analysis, and experimental methods. The chapters also discuss the origins of mucins in jellyfish, feces, saliva and salivary glands, bronchi, stomach, and cervical tract; organic synthesis of peptides glycosylated at specific sites; analysis of mucin gene expression and methylation-specific electrophoresis of genes; imaging of mucin networks by AFM; and experimental methods using supported molecular matrix electrophoresis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Mucins: Methods and Protocols is a valuable resource for researchers who want to obtain in-depth insight on how to more thoroughly conduct mucin research in their laboratories. 432 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This collection explores the use of pluripotent cells to generate early embryo-like structures in vitro, with the resultant 3D embryo-like structures in vitro phenocopying many of the developmental landmarks and 3D architectures seen normally in vivo. The protocols gathered herein cover diverse aspects of the topic and exemplify some of the key developments and improvements in the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 336 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition explores standard laboratory protocols and methodology commonly used in basic and translational studies in the field of rheumatoid arthritis (RA) treatment. Chapters detail including basic RA models, evaluation of disease activity and immunological status, systemic drug delivery, and new research tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. 368 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware 355 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 188 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volume explores the latest ways to detect hypoxia in the context of cancer, including techniques to study gene expression changes, protein responses, and cellular adaptations to low-oxygen conditions. This book also covers new protocols to characterize hypoxia in tumors with high spatial resolution and novel therapeutic approaches that consider the complex microenvironments of solid tumors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 280 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This detailed volume explores techniques for the isolation, purification, and establishment of in vitro germ cell (GS) systems at different stages of development in a variety of organisms, including chickens, mice, rats, and humans. The book additionally describes cutting-edge analytic and informatic tools to study germ cell development at the single cell level and meiotic recombination. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 300 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -A cornerstone of the professional therapeutic relationship and a vital prerequisite to effective treatment, the diagnostic interview sets the tone for interventions that follow. This welcome update to the authoritative textbook includes coverage of foundational and advanced skills and strategies for effective clinical and diagnostic interviewing. Completely revised and updated to correspond to the DSM-5-TR and to reflect the latest innovations in theory and evidence-based practice, this instructive book offers a wealth of useful interviewing strategies and techniques. Specific interviewing approaches are discussed for diverse settings and diverse clients across a variety of presenting problems and mental disorders, as well as keys to ensuring that the interview process is effective and clinically sound. As with its predecessors, this volume emphasizes the value of the interview as the foundation for treatment planning, intervention, and the healing therapeutic relationship.Among the topics covered:Ethical and professional issues.Interviewing strategies, rapport, and empathy.Presenting problem, history of presenting problem, and social history.The mental status examination.Consideration of neuropsychological factors in interviewing.Specific disorders including depressive disorders, bipolar disorders, anxiety disorders, obsessive-compulsive and related disorders, trauma and stressor-related disorders, dissociative disorders, somatic symptom disorders, eating disorders, sexual dysfunctions and gender dysphoria, substance use disorders, personality disorders, and schizophrenia spectrum disorders.Special populations, including children, older adults, interviewing in health, medical, and integrated care settings, and interviewing individuals at risk for suicide.Previous editions of Diagnostic Interviewing have been used in the training and education of diverse mental health professionals including psychologists, psychiatrists, social workers, marriage and family therapists, and professional counsellors. This Sixth Edition will continue this tradition, aiding students, new practitioners, and seasoned clinicians. 616 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This second edition provides new and updated methods detailing techniques and state-of-the-art approaches on the structure and function of plant endoplasmic reticulum (ER). Chapters guide readers through modern microscopy techniques, software protocols, purification, and analysis of ER membrane structure. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 452 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 396 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volume explores the latest advancements in mucin research. The chapters in this book are organized into 8 parts and cover a wide range of topics such as mucin extraction, isolation, physicochemical property analysis, and experimental methods. The chapters also discuss the origins of mucins in jellyfish, feces, saliva and salivary glands, bronchi, stomach, and cervical tract; organic synthesis of peptides glycosylated at specific sites; analysis of mucin gene expression and methylation-specific electrophoresis of genes; imaging of mucin networks by AFM; and experimental methods using supported molecular matrix electrophoresis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 432 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This fully updated volume collects laboratory techniques and tests to assess the risks to embryo-fetal development from drug exposure during early developmental stages. Beginning with a section detailing basic principles of teratogenicity, the book continues with application of in vitro models for teratogenic screening to predict toxicity in embryonic animals and humans, as well as a variety of well-established in vivo animal model tests, which offer solutions for evaluating the efficacy and the teratogenicity of compounds in preclinical trials before human safety testing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Teratogenicity Testing: Methods and Protocols, Second Edition serves as an ideal guide to tests that can be performed in cells, organs, tissues, and animal models for evaluating toxicity and/or safety of compounds in early developmental stages with the goal of estimating, preventing, or minimizing the teratogenic potential of drugs. 627 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides an overview of the existing predictive microbiology software tools, describing the main characteristics of use and application. Chapters will guide readers through tool features and functionalities, and specific case studies. Written in the format of theMethods and Protocols in Food Scienceseries, the chapters include an introduction to the respective topic, list necessary materials and reagents, detail well established and validated methods for readily reproducible laboratory protocols and contain notes on how to avoid or solve typical problems.Authoritative and cutting-edge,Basic Protocols in Predictive Microbiology Softwares aims to ensure successful results in the further study of this vitalfield. 296 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides a comprehensive introduction to methods and procedures related to the preparation, characterization, and main result interpretations of methods used for studying molecular interactions in Food Science. Chapters detail spectroscopic methods and non-spectroscopic methods used to assess the molecular interactions between food constituents. Written in the format of theMethods and Protocols in Food Scienceseries, the chapters include an introduction to the respective topic, list necessary materials and reagents, detail well established and validated methods for readily reproducible laboratory protocols and contain notes on how to avoid or solve typical problems.Authoritative and cutting-edge,Characterization of Molecular Interactions aims to give to the reader sufficient guidance into the different principles and methods. 216 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -A cornerstone of the professional therapeutic relationship and a vital prerequisite to effective treatment, the diagnostic interview sets the tone for interventions that follow. This welcome update to the authoritative textbook includes coverage of foundational and advanced skills and strategies for effective clinical and diagnostic interviewing. Completely revised and updated to correspond to the DSM-5-TR and to reflect the latest innovations in theory and evidence-based practice, this instructive book offers a wealth of useful interviewing strategies and techniques. Specific interviewing approaches are discussed for diverse settings and diverse clients across a variety of presenting problems and mental disorders, as well as keys to ensuring that the interview process is effective and clinically sound. As with its predecessors, this volume emphasizes the value of the interview as the foundation for treatment planning, intervention, and the healing therapeutic relationship.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 616 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This detailed volume explores various methods through which nutrient sensing can be studied, particularly in multicellular eukaryotes. Beginning with a section on the monitoring of cellular energy status by sensing of adenine nucleotides, the book continues by delving into numerous techniques for researching both intracellular and extracellular nutrient sensing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 344 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores the latest advancements and methodologies in studying Hox genes; gene regulation; and Hox gene expression, function, and regulation in various model organisms. The chapters in this book cover topics such as high-resolution imaging and gene editing, bioinformatics approaches ad chromatin analysis, Spatial technology (Curio), ChIP-Seq, and ATAC-Seq. There are also chapters on data analysis, variant calling, gene expression analysis using SmFISH, and microscopy-based approaches to study HOX gene function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on trouble shooting and avoiding known pitfalls.Cutting-edge and comprehensive, HOX Genes: Methods and Protocols is a valuable resource for the researchers in fields of biochemistry, developmental biology, molecular biology, and biomedicine. This book also shows scientists and researchers the critical roles that Hox genes play in development, evolution, and utility as model systems for studying basic molecular biology. 265 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volume provides researchers and students with an introduction to the identification and transmission of plant viruses as well as providing them with practical methodologies that demonstrate the detection of plant viruses through immunological and molecular methods.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 320 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volume provides an overview of the existing predictive microbiology software tools, describing the main characteristics of use and application. Chapters will guide readers through tool features and functionalities, and specific case studies. Written in the format of the Methods and Protocols in Food Science series, the chapters include an introduction to the respective topic, list necessary materials and reagents, detail well established and validated methods for readily reproducible laboratory protocols and contain notes on how to avoid or solve typical problems.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 296 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This detailed book presents state-of-the-art protocols from scientists actively working in autophagy research, a crucial cellular process that regulates numerous cellular functions. The volume explores aspects of autophagy research where a better understanding of its role is vitally important, such as in the development and disease states. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 332 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volume details methods to detect Rhinovirus (RV) in clinical samples, propagation and purification of virus, in vivo and in vitro model systems, and the newer molecular tools that are useful for the studying the RV infection and pathogenesis. Chapters guide readers through a greater of understanding of RV pathogenesis in COPD, childhood asthma development and protective innate immunity. However, there is much to learn about RV infection in order to develop therapies to treat asthma and COPD exacerbations, prevent development of asthma and treat common colds. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 204 pp. Englisch.