springer us nov 2023 (26 risultati)

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Executives are under escalating pressure to deliver increasing profits every quarter, while technology advances at an increasing rate. In such a pressure-cooker environment, leaders must hard-wire their organizations to be mindful of costs while simultaneously becoming more flexible and faster at planning and executing the change programs required by innovation and the shifting demands of the customer. The companies that successfully innovate and stay at the top of their industries have one thing in common - they learn fast and manage change well.Charting Change provides a set of visual tools that will help you build a coherent approach to change, identifying both the things that will help make the effort successful, and those people and barriers that will try to block the way. Leaders that read and absorb this book will be able to make productive use of the tools to drive buy-in, alignment, and successful change outcomes across the organization, its systems, and its culture. This new edition features new topics such as architecting for change, overcoming resistance to change, and implementing change at the pace as well as new visual tools such as the Organizational Agility Framework. This book will help leaders and managers visualize, plan, and execute change and transformation in a more accessible, visual, and collaborative way. Once you are done reading Charting Change you will approach any big change with confidence. 287 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides basic insight and protocols relating to endophytic microbes. Chapter are divided into five major sections detailing basic isolation, bioactive metabolites production. endophytism, isolation and identification of endophytes, bioactive potentials, and screening of metabolites.Authoritative and cutting-edge,Endophytic Microbes: Isolation, Identification, and Bioactive Potentials aims to provide comprehensive and accessible methods to undergraduate, graduate, and established scientist. 288 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed volume provides methods to guide assay development, procedures designed to investigate the chemokine and glycosaminoglycan (GAG) networks, as well as their interactions, in a wide range of organs and tissues in disease and in health. The initial chapters in this book present in vivo models used to examine the roles of chemokines and GAGs in normal physiology and in the pathophysiology of disease. The book then explores present cell- and tissue-based in vitro assays to examine chemokine:GAG interactions. Finally, analytic approaches are presented that provide assays for measuring GAGs, chemokines, and cellular responses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Chemokine-Glycosaminoglycan Interactions: Methods and Protocols serves as an ideal guide for researchers seeking to analyze chemokine and GAG functions, interactions, and molecular mechanisms in vivo and in vitro. 280 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides researchers with the most updated understanding of the basics of West Nile Virus (WNV). Chapters focus on the biology, virology, epidemiology, pathogenesis, as well as the step-by-step molecular, cellular, and statistical methods to study WNV infection in cell culture, mosquitos, animal models, and human clinical specimen. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, West Nile Virus: Methods and Protocols aims to be a valuable resource for all researchers interested in learning more about this important and developing field. 240 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed volume explores the most recent methodologies and applications of stable isotope labeling by amino acids in cell culture (SILAC). With the development of new technologies in the field of mass spectrometry and bioinformatics, SILAC-based methods have become the first tool of choice in quantitative proteomics either on its own or in combination with other more recently developed approaches, which is elucidated in the chapters of this book. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, SILAC: Methods and Protocols serves as an ideal guide for researchers working in the area of functional proteomics and other aspects of protein science. 300 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume details novel and popular approaches to decipher theUbiquitin Code. Chapters guide readers through ubiquitin chain complexity,methods to generate non-hydrolysable ubiquitin-NEDD8,afully synthetic method for the linear synthesis of fluorescently labelled rhodamine LC3A and LC3B,aHECTligase-mediated ubiquitylationin vitro,aRNAi-based screeningto identify DUBs,aYuh1 activityassay,a pull-down to isolateK48 and K63 chainsusingspecific nanobodies,identification of NeDDylated protein based in aNEDD8R74Kmutant,in vivoidentificationof NEDDylated proteins fromliver diseases,UbL-ID method to identifyubiquitin-like modifiedproteins,a LC3/GABARAP capturing system, andmethods to isolate peptides that are closely associated with proteasomes or trapped inside the core particle of proteasomes in eukaryotic cells,among others.Written in the successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,The Ubiquitin Codeaimstoto be auseful practical guide to researches to help further their study in this field. 260 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book aims to be an introduction to haplotype information for both the wet and dry labs. Chapters detail co-barcoding and linked-reads based methods, third generation sequencing based methods, Hi-C based methods, single-cell and Strand-seq, and methods for using haplotype data once obtained. Written in the successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,Haplotyping: Methods and Protocols aims to provide compréhensive and accessible methods to undergraduate, graduate, and established scientists. 312 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware 508 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second editionprovides state-of-the-art and novel methods on antibiotic isolation and purification, identification of antimicrobial killing mechanisms, as well as methods for the analysis and detection of microbial responses and adaptation strategies. Antibiotics: Methods and Protocols, SecondEdition, guides readers through updated and entirely new chapters on production and design, mode of action, and response and resistance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Antibiotics: Methods and Protocols, Second Edition aims to inspire scientific work in the exciting field of antibiotic research. 420 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition compiles state-of-art protocols to study the molecular mechanisms that determine proteome composition and contribute to maintaining protein homeostasis. Chapters describe methods that range from the in vitro analysis of enzyme cascades to real-time in vivo studies. Written in the successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,Plant Proteostasis: Methods and Protocols, Second Edition aims to be auseful practical guide to researches to help further their study in this field. 432 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed book brings together a collection of methodologies, from the most basic to the more complex, that provides researchers with a platform they can use to embark on a cartilage research career. To aid in the search for novel therapies for cartilage regeneration, this volume addresses 3D cartilage models, challenges associated with RNA and protein extraction, imaging, gene transfer, as well as stable differentiation and variations in cell phenotype from different tissue origins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Cartilage Tissue Engineering serves as an ideal guide for researchers working to advance the vital study of cartilage biology and repair. 412 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The second edition of this volume provides a comprehensive update of this key method on gel-based proteomics. Chapters present an introduction into the development of methods on principles of differential protein labeling and two-dimensional gel electrophoresis, techniques on optimized proteomic workflows using advanced mass spectrometry for protein identification, and the application of those methods in basic biological research, pathobiology and applied biomarker discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Difference Gel Electrophoresis: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field. 500 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition volume expands on the previous edition with updated methodologies and practical tips to overcome obstacles associated with experimentation pertaining to chemistry, biology, physiology, pathology, medical and dental sciences, and pharmacology of CCN proteins. The chapters in this book cover topics such as CCN4 immunofluorescence for tissue microarray; utilizing public molecular biological databases for CCN family research; the effects of CCN4 on pancreatic beta cell proliferation; gene expression analysis of CCNs; novel cell biological assays for measuring bone remodeling activities of CCN proteins; and the function of CCN2 in tubular epithelium cells with a focus on renal fibrogenesis.Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive,CCN Proteins: Methods and Protocols, Second Editionis a valuable resource for experienced CCN researchers looking for new approaches and novice researchers just starting out in the field of CCN research. 444 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition volume provides new and updated chapters detailing simple and accessible experiment protocols to explore thymus biology.Chapters are divided into three parts presenting short reviews, analysis strategies, protocols for cell preparation, flow cytometry analyses,Innate Lymphoid Cells (ILC), mouse T-cell development,antigen receptor-less cousins of T cells,bone marrow chimeras,thymic stroma,and multiple aspects of thymocyte biology.Written in the successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,T-Cell Development: Methods and Protocols, Second Edition aims to be auseful practical guide to help readers overcome obstacles associated with experimental approaches of T-cell development. 396 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book aims to advance the understanding of deubiquitinases (DUBs)and DUB-like enzymes. Chapters detail methods used to identify, classify,and biochemically characterize DUBs along with approaches thatenable both the determination and alteration of DUB biological function. Written in the format of the highly successfulMethods in Molecular Biologyseries, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.Authoritative and cutting-edge,Deubiquitinases: Methods and Protocols aims to be auseful and practical guide to new researchers and experts looking to expand their knowledge. 316 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores the latest techniques used to study the human brain towards understanding Alzheimer's Disease and related neurodegenerative disorders. Contributed to by world-renowned experts, the chapters in this book are divided into five parts. Part One discusses human post-mortem brain preparations including single-cell isolation and use of specialized imaging. Part Two talks about neural cellular models using primary and human induced pluripotent stem cells to model aspects of the human brain. Part Three details nucleic acid analyses including transcriptomic and somatic genomic changes, and Part Four discusses lipid analyses via mass spectrometry. Lastly, Part Five covers protein analyses, particularlyAbetaand Tau. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, andtips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Alzheimer's Disease: Methods and Protocols is a valuable tool for all researchers who want to expand their knowledge and understanding of this disease and other related dementias. 356 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed volume explores a variety of techniques used to study lineage commitment in stem cells. Further elucidation of the process that stem cells undergo on their way to becoming more specified cell types is vital for a more complete understanding of cell biology and overall physiology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Stem Cells and Lineage Commitment: Methods and Protocols serves as an ideal guide for experts and novices in the field of stem cell biology. 240 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed book presents a comprehensive collection of state-of-the-art protocols on muscular dystrophy therapeutics, covering therapeutics using antisense oligonucleotides, gene replacement, genome editing, small molecules, stem cells, and antibodies. Written by leaders in the field, the volume explores techniques that are currently in use and are starting an exciting therapeutic revolution in muscular dystrophy. As a part of the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Muscular Dystrophy Therapeutics: Methods and Protocols serves as an ideal resource to inspire readers and provide tips, strategies, and advice to develop new therapeutic technologies for this group of diseases. 596 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume covers the latest advancements in the study of ciliary complexity. Protocols cover genomic, proteomic, imaging, and functional analysis of different ciliated tissues and their wide applicability in cilia biology. Chapters in this book primarily focus on methods to study multiciliated cells, and discuss topics such as SARS-CoV-2 infections of human primary nasal multiciliated epithelial cells; expansion microscopy of ciliary proteins; live-imaging centriole amplification in mouse brain multiciliated cells; biophysical properties of cilia motility; and mucociliary transport device construction. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Cilia: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field. 292 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed volume explores methods currently used to investigate the cell wall of various bacterial species and pathogens. By using a combination of genetic, molecular, biochemical, and cytological techniques, the protocols address many fundamental questions involving the composition, biosynthesis, and regulation of bacterial peptidoglycan. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls.Authoritative and practical, The Bacterial Cell Wall: Methods and Protocols provides current and future researchers with a compilation of many of the most important and useful procedures in a single resource. 252 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This fully revised new edition explores advances in the prevention and treatment of oral diseases. Beyond the updated chapters, the book delves into regenerative biology, gene editing and the use of CRISPR in oral biology, as well as histone acetylation and deacetylation methods, further reflecting advances in the application of molecular techniques to oral biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Oral Biology: Molecular Techniques and Applications, Third Edition serves as an ideal basic resource not only for new researchers but also for experienced scientists wishing to expand their research platform into new areas of this vital field. 548 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book aims to provide methods, protocols, and discussion topics for those who wish to examine in depth the molecular mechanisms of adaptation and versality of bacteria and would like to envisage their evolution responses in the fast changing Antropocene.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Pseudomonas aeruginosa: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. 256 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware 528 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed volume provides practical guidance on techniques in plant-virus interaction research, from targeting specific molecular interactions within the virus-host interactome to the identification of the complete virus-host protein-protein interaction network. After chapters on acquiring the necessary molecular tools, the book continues with biochemical and genetic approaches to confirming protein-protein interactions both in vivo and in vitro, procedures and protocols for assessing replication, translation, viral genome movement, and insect transmission, as well as techniques for detecting multiple molecular interactions between the host and the virus and monitoring immune hubs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and comprehensive, Plant-Virus Interactions serves as a valuable resource for understanding the protein-protein interaction network between the virus and the host, crucial for comprehending the life cycle of a virus and for developing strategies for broad-spectrum and long-lasting resistance against viral infections. 328 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides methods to study ice-binding proteins (IBPs), and applications involving these proteins.Chapters are divided into three parts describing identifying, isolating, and purifying ice-binding proteins, characterize both IBPs and ice-nucleating proteins (INPs), and synthesize mimics of IBPs, as well as applications involving these proteins.Written in the format of the highly successfulMethods in Molecular Biologyseries, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.Authoritative and cutting-edge,Ice Binding Proteins: Methods and Protocolsaimsto provideboth experimental and simulations protocols to help new researchers, break-the-ice, and enter this exciting field, while also supporting established researchers broaden the scope of theirinvestigations. 244 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition details new and updated protocols that cover techniques used to study secretion systems. Chapters focus on identifying and localizingthe different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as using reporters to follow effector transport.Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,Bacterial Secretion Systems: Methods and Protocols- Second Editionaimsto be auseful and practical guide to new researchersand experts looking to expand their knowledge. 632 pp. Englisch.