9780879693855 - strategies for protein purification and characterization: a laboratory course manual : biosupplynet source book 99 (6 risultati)
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Strategies for Protein Purification and Characterization
Lin Sue-Hwa Brennan William A. Knurth Mark Burgess Richard R. Kadonaga James T. Marshak Daniel
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Strategies for Protein Purification and Characterization
Sue-Hwa Lin William A. Brennan Mark Knurth Richard R. Burgess James T. Kadonaga Daniel Marshak
Lingua: Inglese
Editore: Cold Spring Harbor Laboratory Press CSHL Press 1995
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Condizione: New. Print on Demand pp. 416.
Strategies for Protein Purification and Characterization
Lin Sue-Hwa Brennan William A. Knurth Mark Burgess Richard R. Kadonaga James T. Marshak Daniel
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Condizione: New. PRINT ON DEMAND pp. 416.
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Da: AHA-BUCH GmbH, Einbeck, GermaniaAHA-BUCH GmbH
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Taschenbuch. Condizione: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Investigators who have identified and cloned a gene of interest often want to isolate and characterize the protein product, yet the methods required are no-toriously tricky for the inexperienced. For the past four years, a course has be…en held at Cold Spring Harbor Laboratory to teach scientists how to execute the major protein techniques by applying them to four distinct, representative types of molecule: a regulatory protein, a DNA-binding protein, a recombinant protein, and a membrane-bound receptor. This course has now been adapted in the form of a laboratory manual that covers a variety of bulk fractionation, electrophoretic, and chromatographic techniques. Step-by-step protocols are accompanied by troubleshooting advice and guidance on generalizing the techniques for other classes and types of protein. The emphasis throughout is on strategies for purification and characterization rather than automated instrumental analysis. After years of rigorous testing, these techniques are robust and reliable, and are presented here with the clarity and completeness for which Cold Spring Harbor manuals are celebrated. The book is invaluable for specialists in genetics, microbiology, neuroscience, and cell biology who wish to develop expertise in working with proteins.
