9780896034570 - Transgenic Xenopus: Microinjection Methods and Developmental Neurobiology: 28 di Soreq, Hermona; Seidman, Shlomo (23 risultati)

gebundene Ausgabe. Condizione: Gut. 198 Seiten Das hier angebotene Buch stammt aus einer teilaufgelösten wissenschaftlichen Bibliothek und trägt die entsprechenden Kennzeichnungen (Rückenschild, Instituts-Stempel.); Einbandkanten sind leicht bestoßen; der Buchzustand ist ansonsten ordentlich und dem Alter entsprechend gut. Sprache: Englisch Gewicht in Gramm: 540.

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Condizione: Used. pp. 224.

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Condizione: New.

Condizione: Used. pp. 224.

Condizione: Used. pp. 224.

Hardcover. Condizione: new. Hardcover. Two distinguished international experts offer a fully illustrated, thoroughly referenced guide to the use of microinjected Xenopus embryos to study the role and spatiotemporal regulation of nervous system proteins during vertebrate development. Drs. Seidman and Soreq include all the basic scientific background and protocols needed to consider and exploit the vast potential of this model system. They provide an outline of Xenopus development, detailed methods for in vitro fertilization and microinjection, and protocols and references for a variety of biochemical and histochemical techniques to analyze the results of a microinjection experiment. They also present extensive experimental data illustrating the use of enzyme activity assays, gel electrophoresis, density ultracentrifugation, EAIA, RT-PCR, cytochemical and immunocytochemical staining, and electron microscopy. Transgenic Xenopus demonstrates convincingly the special utility of Xenopus embryo microinjection as a sophisticated and versatile tool for studying nervous system proteins. The need to better understand the molecular, b- chemical, and cellular processes by which a developing neuronal system unfolds has led to the development of a unique set of experimental tools and organisms. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.

Condizione: New. In.

Condizione: New. This demonstration of the special utility of Xenopus embryo microinjection for the study of nervous system proteins opens up a range of possibilites for researchers who have exhausted the potential of the Xenopus oocyte. Series: Neuromethods. Num Pages: 198 pages, biography. BIC Classification: PSAN; PSVW3. Category: (P) Professional & Vocational; (UP) Postgraduate, Research & Scholarly; (UU) Undergraduate. Dimension: 235 x 155 x 20. Weight in Grams: 543. . 1996. 1997th Edition. hardcover. . . . .

Hardcover. Condizione: Like New. Like New. book.

Hardcover. Condizione: Brand New. 1st edition. 198 pages. 9.50x6.25x0.75 inches. In Stock.

Condizione: New. This demonstration of the special utility of Xenopus embryo microinjection for the study of nervous system proteins opens up a range of possibilites for researchers who have exhausted the potential of the Xenopus oocyte. Series: Neuromethods. Num Pages: 198 pages, biography. BIC Classification: PSAN; PSVW3. Category: (P) Professional & Vocational; (UP) Postgraduate, Research & Scholarly; (UU) Undergraduate. Dimension: 235 x 155 x 20. Weight in Grams: 543. . 1996. 1997th Edition. hardcover. . . . . Books ship from the US and Ireland.

Condizione: As New. Unread book in perfect condition.

Hardcover. Condizione: new. Hardcover. Two distinguished international experts offer a fully illustrated, thoroughly referenced guide to the use of microinjected Xenopus embryos to study the role and spatiotemporal regulation of nervous system proteins during vertebrate development. Drs. Seidman and Soreq include all the basic scientific background and protocols needed to consider and exploit the vast potential of this model system. They provide an outline of Xenopus development, detailed methods for in vitro fertilization and microinjection, and protocols and references for a variety of biochemical and histochemical techniques to analyze the results of a microinjection experiment. They also present extensive experimental data illustrating the use of enzyme activity assays, gel electrophoresis, density ultracentrifugation, EAIA, RT-PCR, cytochemical and immunocytochemical staining, and electron microscopy. Transgenic Xenopus demonstrates convincingly the special utility of Xenopus embryo microinjection as a sophisticated and versatile tool for studying nervous system proteins. The need to better understand the molecular, b- chemical, and cellular processes by which a developing neuronal system unfolds has led to the development of a unique set of experimental tools and organisms. Shipping may be from our Sydney, NSW warehouse or from our UK or US warehouse, depending on stock availability.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The need to better understand the molecular, b- chemical, and cellular processes by which a developing neuronal system unfolds has led to the development of a unique set of experimental tools and organisms. Special emphasis was devoted to allowing us access, at the ear- est stages, to the genomic basis underlying the system's ultimate complexity, as exhibited once its structures are fully formed. Yet, nerve cells are anatomically, physiolo- cally, and biochemically diverse. The multitude of d- tinctly different routes for their development thus makes the developing nervous system especially intriguing for molecular neurobiologists. In particular, the demands of modern molecular neuroscience call for the establishment of efficient yet versatile systems for studying these c- plex processes. Transgenic embryos of the frog Xenopus laevis offer an excellent system for approaching neuroscientific issues. Insertion of foreign genes is performed simply, by mic- injection under binocular observation; hundreds of in vitro-fertilized embryos can be microinjected in one experiment. Embryos develop in tap water, at room t- perature, and within a few days become independent swimming tadpoles with fully functioning neuromus- lar systems. Being relatively small, these organisms are amenable to detailed analyses at the levels of mRNA, protein, and cell. Their rapid development permits the study of morphogenetic processes involved in early development, such as myogenesis and neural induction, as well as those involved in organogenesis and formation of the brain, the musculature, and the interconnections between them. Foreign DNA remains predominantly extrachromosomal. 224 pp. Englisch.

Gebunden. Condizione: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. The need to better understand the molecular, b- chemical, and cellular processes by which a developing neuronal system unfolds has led to the development of a unique set of experimental tools and organisms. Special emphasis was devoted to allowing us access.

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -The need to better understand the molecular, b- chemical, and cellular processes by which a developing neuronal system unfolds has led to the development of a unique set of experimental tools and organisms. Special emphasis was devoted to allowing us access, at the ear- est stages, to the genomic basis underlying the system¿s ultimate complexity, as exhibited once its structures are fully formed. Yet, nerve cells are anatomically, physiolo- cally, and biochemically diverse. The multitude of d- tinctly different routes for their development thus makes the developing nervous system especially intriguing for molecular neurobiologists. In particular, the demands of modern molecular neuroscience call for the establishment of efficient yet versatile systems for studying these c- plex processes. Transgenic embryos of the frog Xenopus laevis offer an excellent system for approaching neuroscientific issues. Insertion of foreign genes is performed simply, by mic- injection under binocular observation; hundreds of in vitro-fertilized embryos can be microinjected in one experiment. Embryos develop in tap water, at room t- perature, and within a few days become independent swimming tadpoles with fully functioning neuromus- lar systems. Being relatively small, these organisms are amenable to detailed analyses at the levels of mRNA, protein, and cell. Their rapid development permits the study of morphogenetic processes involved in early development, such as myogenesis and neural induction, as well as those involved in organogenesis and formation of the brain, the musculature, and the interconnections between them. Foreign DNA remains predominantly extrachromosomal. 224 pp. Englisch.

Buch. Condizione: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - The need to better understand the molecular, b- chemical, and cellular processes by which a developing neuronal system unfolds has led to the development of a unique set of experimental tools and organisms. Special emphasis was devoted to allowing us access, at the ear- est stages, to the genomic basis underlying the system's ultimate complexity, as exhibited once its structures are fully formed. Yet, nerve cells are anatomically, physiolo- cally, and biochemically diverse. The multitude of d- tinctly different routes for their development thus makes the developing nervous system especially intriguing for molecular neurobiologists. In particular, the demands of modern molecular neuroscience call for the establishment of efficient yet versatile systems for studying these c- plex processes. Transgenic embryos of the frog Xenopus laevis offer an excellent system for approaching neuroscientific issues. Insertion of foreign genes is performed simply, by mic- injection under binocular observation; hundreds of in vitro-fertilized embryos can be microinjected in one experiment. Embryos develop in tap water, at room t- perature, and within a few days become independent swimming tadpoles with fully functioning neuromus- lar systems. Being relatively small, these organisms are amenable to detailed analyses at the levels of mRNA, protein, and cell. Their rapid development permits the study of morphogenetic processes involved in early development, such as myogenesis and neural induction, as well as those involved in organogenesis and formation of the brain, the musculature, and the interconnections between them. Foreign DNA remains predominantly extrachromosomal.