Isbn: 9781936113583 - calcium techniques: a laboratory manual (16 risultati)

paperback. Condizione: Fine. Sealed in plastic.

Condizione: New.

Paperback or Softback. Condizione: New. Calcium Techniques: A Laboratory Manual. Book.

Paperback. Condizione: New.

Condizione: New.

Paperback. Condizione: Brand New. lab manual edition. 592 pages. 12.00x10.00x1.00 inches. In Stock.

Condizione: New. pp. 608 62 Illus. (4 Col.).

Condizione: New. pp. 608 Index.

Paperback. Condizione: New.

Paperback. Condizione: new. Paperback. Life begins with a surge of calcium ions (Ca2+) at fertilization, and thereafter, Ca2+ signaling influences nearly every aspect of mammalian development and physiology, from gene expression and cell proliferation to muscle contraction and nerve impulses. To create spatiotemporally distinct Ca2+ signals, cells use a variety of mechanisms to recognize, transport, and buffer Ca2+. Thus, a diverse range of reliable experimental techniques is necessary to study the movement of Ca2+ and the various effectors involved. This laboratory manual provides step-by-step protocols for studying many facets of Ca2+ signaling, as well as background information on the principles and applications of the techniques. Contributors discuss how to use fluorescent, luminescent, and genetically encoded Ca2+ probes in conjunction with state-of-the-art imaging modalities to characterize Ca2+ signals. Electrophysiological measurements of Ca2+ channel activity are described, as are radioactive Ca2+ flux assays and methods to investigate signaling mediated by specific Ca2+-mobilizing messengers (IP3, cADPR, and NAADP). Techniques to modulate and suppress intra- and intercellular signals are also provided. Each protocol is complete with a list of required materials, detailed recipes for media and reagents, and troubleshooting advice. Specific chapters are devoted to Ca2+ signaling techniques in non-mammalian systems, such as plants, yeast, zebrafish, and Xenopus. Methods for assessing Ca2+-binding kinetics and strategies for developing mathematical models of Ca2+ signaling are also included. Thus, this manual is a comprehensive laboratory resource for biochemists, cell and developmental biologists, and physiologists who are using or looking to expand their repertoire of Ca2+ techniques. Signaling by calcium ions is an important regulator of muscle contraction, nerve impulses, fertilization, and numerous other cellular processes. This book explores the experimental techniques used to monitor movement of calcium ions into/out of cells and between subcellular compartments, as well as analyse its effects on the intracellular targets that mediate its effects. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.…

Paperback. Condizione: New.

Taschenbuch. Condizione: Wie neu. Gebraucht - Wie neu -Life begins with a surge of calcium ions (Ca2+) at fertilization, and thereafter, Ca2+ signaling influences nearly every aspect of mammalian development and physiology, from gene expression and cell proliferation to muscle contraction and nerve impulses. To create spatiotemporally distinct Ca2+ signals, cells use a variety of mechanisms to recognize, transport, and buffer Ca2+. Thus, a diverse range of reliable experimental techniques is necessary to study the movement of Ca2+ and the various effectors involved. This laboratory manual provides step-by-step protocols for studying many facets of Ca2+ signaling, as well as background information on the principles and applications of the techniques. Contributors discuss how to use fluorescent, luminescent, and genetically encoded Ca2+ probes in conjunction with state-of-the-art imaging modalities to characterize Ca2+ signals. Electrophysiological measurements of Ca2+ channel activity are described, as are radioactive Ca2+ flux assays and methods to investigate signaling mediated by specific Ca2+-mobilizing messengers (IP3, cADPR, and NAADP). Techniques to modulate and suppress intra- and intercellular signals are also provided. Each protocol is complete with a list of required materials, detailed recipes for media and reagents, and troubleshooting advice. Specific chapters are devoted to Ca2+ signaling techniques in non-mammalian systems, such as plants, yeast, zebrafish, and Xenopus. Methods for assessing Ca2+-binding kinetics and strategies for developing mathematical models of Ca2+ signaling are also included. Thus, this manual is a comprehensive laboratory resource for biochemists, cell and developmental biologists, and physiologists who are using or looking to expand their repertoire of Ca2+ techniques.…

Condizione: As New. Unread book in perfect condition.

Condizione: As New. Unread book in perfect condition.

Paperback. Condizione: New.

Paperback. Condizione: new. Paperback. Life begins with a surge of calcium ions (Ca2+) at fertilization, and thereafter, Ca2+ signaling influences nearly every aspect of mammalian development and physiology, from gene expression and cell proliferation to muscle contraction and nerve impulses. To create spatiotemporally distinct Ca2+ signals, cells use a variety of mechanisms to recognize, transport, and buffer Ca2+. Thus, a diverse range of reliable experimental techniques is necessary to study the movement of Ca2+ and the various effectors involved. This laboratory manual provides step-by-step protocols for studying many facets of Ca2+ signaling, as well as background information on the principles and applications of the techniques. Contributors discuss how to use fluorescent, luminescent, and genetically encoded Ca2+ probes in conjunction with state-of-the-art imaging modalities to characterize Ca2+ signals. Electrophysiological measurements of Ca2+ channel activity are described, as are radioactive Ca2+ flux assays and methods to investigate signaling mediated by specific Ca2+-mobilizing messengers (IP3, cADPR, and NAADP). Techniques to modulate and suppress intra- and intercellular signals are also provided. Each protocol is complete with a list of required materials, detailed recipes for media and reagents, and troubleshooting advice. Specific chapters are devoted to Ca2+ signaling techniques in non-mammalian systems, such as plants, yeast, zebrafish, and Xenopus. Methods for assessing Ca2+-binding kinetics and strategies for developing mathematical models of Ca2+ signaling are also included. Thus, this manual is a comprehensive laboratory resource for biochemists, cell and developmental biologists, and physiologists who are using or looking to expand their repertoire of Ca2+ techniques. Signaling by calcium ions is an important regulator of muscle contraction, nerve impulses, fertilization, and numerous other cellular processes. This book explores the experimental techniques used to monitor movement of calcium ions into/out of cells and between subcellular compartments, as well as analyse its effects on the intracellular targets that mediate its effects. Shipping may be from our Sydney, NSW warehouse or from our UK or US warehouse, depending on stock availability.…