9783034873512 - A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis (13 risultati)

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Taschenbuch. Condizione: Neu. Druck auf Anfrage Neuware - Printed after ordering - 2.1 Introduction . 32 2.2 Technical Procedures . 32 Chemical Synthesis . 35 2.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 . . . . . . . . . . . . . Chemical Synthesis . 38 Binding to anti-CCK antisera and CCK-B receptors . 40 Binding to streptavidin agarose and photoelution . 40 Affinity chromatography . 41 2.4 Troubleshooting. 43 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44 . . . . . . . . . . . . . . . . . Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands . 45 Summary . 45 3.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46 . . . . . . . . . . . . . . . . . 3.2 Technical Procedures . 48 Preparation of a Biotinylated Ligand . 48 Preparation of Membrane Fraction . 50 Solubilization of the PACAP Receptor . 51 Partial Purification of the PACAP Receptor . 52 Affinity Purification of the PACAP Receptor . 54 Final Purification of the PACAP Receptor . 56 Receptor-Binding Assay . 57 3.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 . . . . . . . . . . . . . 3.4 Troubleshooting . 61 Impurity in PACAP27-Cys-NH. 61 2 Low specific activity of the PACAP receptor despite a single band . 62 Acknowledgments . 62 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 . . . . . . . . . . . . . . . . . Photoreactive Biotinylated Peptide Ligands for Affinity Labeling . ' 65 Summary . 65 4.1 Introduction. . . . . . . . . . 66 . 4.2 Technical Procedures . ' 67 Synthesis of a trifunctional photoactivatable biotinylating reagent . 67 Synthesis of photo reactive biotinylated peptide hormones . 67 Site-specific incorporation of biotin and photo labels in separate steps . 69 Photoaffinity labeling . 72 4.3 Results and Discussion . 76 Synthesis of photoactivatable insulins with permanent biotin labels . 76 Applications: Insulin . 78 VI Examples for other applications . 78 4.4 Troubleshooting. 79 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81 . . . . . . . . . . . . . . . . . . .

Taschenbuch. Condizione: Neu. A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis | T. Meier (u. a.) | Taschenbuch | Biomethods | xiv | Englisch | 2012 | Birkhäuser | EAN 9783034873512 | Verantwortliche Person für die EU: Springer Basel AG in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu.

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Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -2.1 Introduction . 32 2.2 Technical Procedures . 32 Chemical Synthesis . 35 2.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 . . . . . . . . . . . . . Chemical Synthesis . 38 Binding to anti-CCK antisera and CCK-B receptors . 40 Binding to streptavidin agarose and photoelution . 40 Affinity chromatography . 41 2.4 Troubleshooting. 43 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44 . . . . . . . . . . . . . . . . . Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands . 45 Summary . 45 3.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46 . . . . . . . . . . . . . . . . . 3.2 Technical Procedures . 48 Preparation of a Biotinylated Ligand . 48 Preparation of Membrane Fraction . 50 Solubilization of the PACAP Receptor . 51 Partial Purification of the PACAP Receptor . 52 Affinity Purification of the PACAP Receptor . 54 Final Purification of the PACAP Receptor . 56 Receptor-Binding Assay . 57 3.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 . . . . . . . . . . . . . 3.4 Troubleshooting . 61 Impurity in PACAP27-Cys-NH. 61 2 Low specific activity of the PACAP receptor despite a single band . 62 Acknowledgments . 62 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 . . . . . . . . . . . . . . . . . Photoreactive Biotinylated Peptide Ligands for Affinity Labeling . ' 65 Summary . 65 4.1 Introduction. . . . . . . . . . 66 . 4.2 Technical Procedures . ' 67 Synthesis of a trifunctional photoactivatable biotinylating reagent . 67 Synthesis of photo reactive biotinylated peptide hormones . 67 Site-specific incorporation of biotin and photo labels in separate steps . 69 Photoaffinity labeling . 72 4.3 Results and Discussion . 76 Synthesis of photoactivatable insulins with permanent biotin labels . 76 Applications: Insulin . 78 VI Examples for other applications . 78 4.4 Troubleshooting. 79 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81 . . . . . . . . . . . . . . . . . . . 240 pp. Englisch.

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Kartoniert / Broschiert. Condizione: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. 1 An Introduction to Avidin-Biotin Technology and Options for Biotinylation.- 1.1 Introduction.- The Avidin-Biotin Interaction.- Avidin.- Streptavidin.- Biotin.- 1.2 Technical Procedures.- Determining Biotin and Biotin Incorporation Levels.- Biotinylation R.

Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -1 An Introduction to Avidin-Biotin Technology and Options for Biotinylation.- 1.1 Introduction.- 1.2 Technical Procedures.- 1.3 Troubleshooting.- Acknowledgments.- References.- 2 Synthesis of Photocleavable Biotinylated Ligands and Application for Affinity Chromatography.- Summary.- 2.1 Introduction.- 2.2 Technical Procedures.- 2.3 Results and Discussion.- 2.4 Troubleshooting.- References.- 3 Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands.- Summary.- 3.1 Introduction.- 3.2 Technical Procedures.- 3.3 Results and Discussion.- 3.4 Troubleshooting.- Acknowledgments.- References.- 4 Photoreactive Biotinylated Peptide Ligands for Affinity Labeling.- Summary.- 4.1 Introduction.- 4.2 Technical Procedures.- 4.3 Results and Discussion.- 4.4 Troubleshooting.- References.- 5 Immunoprecipitation of Biotinylated Cell Surface Proteins.- Summary.- 5.1 Introduction.- 5.2 Technical Procedures.- 5.3 Results and Discussion.- 5.4 Troubleshooting.- Acknowledgments.- References.- 6 Biotinylation and Chemical Cross-Linking of Membrane Associated Molecules.- Summary.- 6.1 Introduction.- 6.2 Technical Procedures.- 6.3 Results and Discussion.- 6.4 Troubleshooting.- Acknowledgments.- References.- 7 Preparation of Biotinylated Lectins and Application in Microtiter Plate Assays and Western Blotting.- Summary.- 7.1 Introduction.- 7.2 Technical Procedures.- 7.3 Results and Discussion.- 7.4 Troubleshooting.- Acknowledgements.- References.- 8 Biotin-Labeling of Poly(ADP-ribose) in Poly(ADP-ribose)-Protein Interactions.- Summary.- 8.1 Introduction.- 8.2 Technical Procedures.- 8.3 Results and Discussion.- 8.4 Troubleshooting.- Acknowledgments.- References.- 9 Preparation and Use of Biotinylated Probes for the Detection and Characterisation of Serine Proteinase and Serine Proteinas Inhibitory Proteins.- Summary.- 9.1 Introduction.- 9.2 Technical Procedures.- 9.3 Results and Discussion.- 9.4 Troubleshooting.- Acknowledgments.- References.- 10 Avidin/Biotin-Mediated Conjugation of Antibodies to Erythrocytes: An Approach for Immunoerythrocyte Exploration in vivo.- Summary.- 10.1 Introduction.- 10.2 Technical Procedures.- 10.3 Results and Discussion.- 10.4 Troubleshooting.- Acknowledgments.- References.- 11 Biotin in vitro Translation: A Nonradioactive Method for the Synthesis of Biotin-Labeled Proteins in a Cell-Free System.- Summary.- 11.1 Introduction.- 11.2 Technical Procedures.- 11.3 Results and Discussion.- 11.4 Troubleshooting.- Acknowledgments.- References.- 12 Nonradioactive Detection of Nucleic Acids with Biotinylated Probes.- Summary.- 12.1 Introduction.- 12.2 Technical procedures.- 12.3 Results and discussion.- 12.4 Troubleshooting.- References.- 13 Biotin-Labeled Riboprobes to Study RNA-Binding Proteins.- Summary.- 13.1 Introduction.- 13.2 Technical Procedures.- 13.3 Results and Discussion.- 13.4 Troubleshooting.- Acknowledgments.- References.- 14 Rapid YAC End Sequencing by Alu-Vector PCR and Biotinylated Primers.- Summary.- 14.1 Introduction.- 14.2 Technical Procedures.- 14.3 Results and Discussion.- 14.4 Troubleshooting.- Acknowledgments.- References.Springer Nature c/o IBS, Benzstrasse 21, 48619 Heek 260 pp. Englisch.