Isbn: 9783844389203 - expression of two insecticidal genes in cotton: development of transgenic cotton with sustained resistance against targeted insect pests (5 risultati)

Taschenbuch. Condizione: Neu. Expression of two insecticidal genes in Cotton | Development of Transgenic Cotton with sustained resistance against targeted insect pests | Allah Bakhsh (u. a.) | Taschenbuch | 176 S. | Englisch | 2011 | LAP LAMBERT Academic Publishing | EAN 9783844389203 | Verantwortliche Person für die EU: preigu GmbH & Co. KG, Lengericher Landstr. 19, 49078 Osnabrück, mail[at]preigu[dot]de | Anbieter: preigu.…

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Spatio-temporal expression of insecticidal gene (Cry1Ac& Cry2A) in pre existing lines of transgenic cotton was studied. Seasonal decline in expression of genes differed significantly among different cotton lines tested in the field conditions. The leaves of the Bt cotton plants were found to have the highest levels of toxin expression followed by squares, bolls, anthers and petals. Expression of the genes decreased consistently with the age of plants. Toxin expression in fruiting parts was not enough to confer full resistance against bollworms. The reduction in efficacy of transgenic cotton plants late in the season was attributed to reduction in promoter activity. For this purpose, Rubisco small subunit promoter was isolated from Gossypium arboreum that was further cloned upstream of Cry1Ac in expression vector pCAMBIA 1301. A local cotton cultivar was transformed with Cry1Ac driven by rbcS promoter. The same cotton cultivar was also transformed with Cry1Ac gene driven by 35SCaMV promoter to compare the expression pattern of insecticidal gene under two different promoters. The results showed that rbcS is an efficient promoter to drive Bt gene expression in cotton. 176 pp. Englisch.…

Condizione: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: Bakhsh AllahDr. Allah Bakhsh was born in Khanewal,(Pakistan)on April 15,1981. He completed four years honor degree in Agricultural sciences majoring in Plant Breeding and Genetics from University of Agriculture, Faisalabad, Pakistan .…

Taschenbuch. Condizione: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Spatio-temporal expression of insecticidal gene (Cry1Ac& Cry2A) in pre existing lines of transgenic cotton was studied. Seasonal decline in expression of genes differed significantly among different cotton lines tested in the field conditions. The leaves of the Bt cotton plants were found to have the highest levels of toxin expression followed by squares, bolls, anthers and petals. Expression of the genes decreased consistently with the age of plants. Toxin expression in fruiting parts was not enough to confer full resistance against bollworms. The reduction in efficacy of transgenic cotton plants late in the season was attributed to reduction in promoter activity. For this purpose, Rubisco small subunit promoter was isolated from Gossypium arboreum that was further cloned upstream of Cry1Ac in expression vector pCAMBIA 1301. A local cotton cultivar was transformed with Cry1Ac driven by rbcS promoter. The same cotton cultivar was also transformed with Cry1Ac gene driven by 35SCaMV promoter to compare the expression pattern of insecticidal gene under two different promoters. The results showed that rbcS is an efficient promoter to drive Bt gene expression in cotton.…

Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Spatio-temporal expression of insecticidal gene (Cry1Ac& Cry2A) in pre existing lines of transgenic cotton was studied. Seasonal decline in expression of genes differed significantly among different cotton lines tested in the field conditions. The leaves of the Bt cotton plants were found to have the highest levels of toxin expression followed by squares, bolls, anthers and petals. Expression of the genes decreased consistently with the age of plants. Toxin expression in fruiting parts was not enough to confer full resistance against bollworms. The reduction in efficacy of transgenic cotton plants late in the season was attributed to reduction in promoter activity. For this purpose, Rubisco small subunit promoter was isolated from Gossypium arboreum that was further cloned upstream of Cry1Ac in expression vector pCAMBIA 1301. A local cotton cultivar was transformed with Cry1Ac driven by rbcS promoter. The same cotton cultivar was also transformed with Cry1Ac gene driven by 35SCaMV promoter to compare the expression pattern of insecticidal gene under two different promoters. The results showed that rbcS is an efficient promoter to drive Bt gene expression in cotton.VDM Verlag, Dudweiler Landstraße 99, 66123 Saarbrücken 176 pp. Englisch.…