ISBN: 9783846526293 - transfection via nucleofection: host cell optimization strategies... (8 risultati)

Condizione: New. pp. 120.

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Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Transfection via Nucleofection is one of the most efficient non- viral method of introducing a foreign gene in to the host cell. Cell-type specific combinations of electrical current and solutions make this technology unique in its ability to transfer polyanionic macromolecules directly into the nucleus. Therefore, even cells with limited potential to divide are made accessible for efficient gene transfer. This monograph shows various optimization parameters and protocols for standardizing nucleofection protocol in goat fibroblast cells using bovine IFN- tagged with EGFP as the model gene construct. This technique can be utilized effectively for the production of animals with better production performances. In addition to the brief protocols of various biotechniques, this book offers a general review of the literatures dealing with the different ways of transfection ,assay of transfection, with the hope that it will give compact information to the researchers and students involved in the reproductive biotechnology. 120 pp. Englisch.…

Condizione: New. Print on Demand pp. 120 2:B&W 6 x 9 in or 229 x 152 mm Perfect Bound on Creme w/Gloss Lam.

Taschenbuch. Condizione: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Transfection via Nucleofection is one of the most efficient non- viral method of introducing a foreign gene in to the host cell. Cell-type specific combinations of electrical current and solutions make this technology unique in its ability to transfer polyanionic macromolecules directly into the nucleus. Therefore, even cells with limited potential to divide are made accessible for efficient gene transfer. This monograph shows various optimization parameters and protocols for standardizing nucleofection protocol in goat fibroblast cells using bovine IFN- tagged with EGFP as the model gene construct. This technique can be utilized effectively for the production of animals with better production performances. In addition to the brief protocols of various biotechniques, this book offers a general review of the literatures dealing with the different ways of transfection ,assay of transfection, with the hope that it will give compact information to the researchers and students involved in the reproductive biotechnology.…

Condizione: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: M.D PratheeshDr. Pratheesh M.D pursuing PhD in Veterinary Physiology from IVRI, India. He completed his BVSc & AH degree from KAU, India in the year 2007. He has obtained his Masters degree in Animal Physiology from NDRI,India. He h.…

Condizione: New. PRINT ON DEMAND pp. 120.

Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Transfection via Nucleofection is one of the most efficient non- viral method of introducing a foreign gene in to the host cell. Cell-type specific combinations of electrical current and solutions make this technology unique in its ability to transfer polyanionic macromolecules directly into the nucleus. Therefore, even cells with limited potential to divide are made accessible for efficient gene transfer. This monograph shows various optimization parameters and protocols for standardizing nucleofection protocol in goat fibroblast cells using bovine IFN- ¿ tagged with EGFP as the model gene construct. This technique can be utilized effectively for the production of animals with better production performances. In addition to the brief protocols of various biotechniques, this book offers a general review of the literatures dealing with the different ways of transfection ,assay of transfection, with the hope that it will give compact information to the researchers and students involved in the reproductive biotechnology.VDM Verlag, Dudweiler Landstraße 99, 66123 Saarbrücken 120 pp. Englisch.…