Isbn: 9786200482471 - bio-analytical method development of antimalarial drug in human plasma: validation for quantitative estimation by using lc-msms (6 risultati)

Paperback. Condizione: Brand New. 128 pages. 8.66x5.91x0.29 inches. In Stock.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -In the present work new, simple reversed-phase LCMS/MS in positive ion mode method was developed and validated for the determination of an anti-malarial in blood plasma. Drug was used as an internal standard. The chromatographic separation was achieved with Zorbax SB-C18, 5.0µm (150\*4.6mm). The separation of Drug H was achieved by gradient elution using organic mixture (Acetonitrile: Methanol::80:20 v/v), buffer solution (10mM Ammonium Formate) and DMSO:: 50:50:0.2 v/v/v. The flow rate is 1.000 ml/min, Injection Volume of 10µl with a run time of 3.0 minutes. The calibration curve of standard Drug H was linear in range 0.1-20.0 ¿g/ml. 5 precision and accuracy batches were run; the coefficient of correlation (r2) was ¿ 0.99. The accuracy of the LLOQ in the standard CC was within ± 20% of the nominal concentration and ± 15% of the nominal concentration for other standards and 75% non- zero calibration standards met the acceptable criteria which indicated the method was precise and accurate. Method validation results have proved the method to be selective, sensitive, precise, accurate and robustness. 128 pp. Englisch.…

Taschenbuch. Condizione: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - In the present work new, simple reversed-phase LCMS/MS in positive ion mode method was developed and validated for the determination of an anti-malarial in blood plasma. Drug was used as an internal standard. The chromatographic separation was achieved with Zorbax SB-C18, 5.0µm (150\*4.6mm). The separation of Drug H was achieved by gradient elution using organic mixture (Acetonitrile: Methanol::80:20 v/v), buffer solution (10mM Ammonium Formate) and DMSO:: 50:50:0.2 v/v/v. The flow rate is 1.000 ml/min, Injection Volume of 10µl with a run time of 3.0 minutes. The calibration curve of standard Drug H was linear in range 0.1-20.0 ¿g/ml. 5 precision and accuracy batches were run; the coefficient of correlation (r2) was ¿ 0.99. The accuracy of the LLOQ in the standard CC was within ± 20% of the nominal concentration and ± 15% of the nominal concentration for other standards and 75% non- zero calibration standards met the acceptable criteria which indicated the method was precise and accurate. Method validation results have proved the method to be selective, sensitive, precise, accurate and robustness.…

Condizione: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: Abraham BincyBINCY ABRAHAM, Master of Pharmacy in Quality Assurance from Jaipur National University, Jaipur, Rajasthan.In the present work new, simple reversed-phase LCMS/MS in positive ion mode method was developed and validated.…

Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -In the present work new, simple reversed-phase LCMS/MS in positive ion mode method was developed and validated for the determination of an anti-malarial in blood plasma. Drug was used as an internal standard. The chromatographic separation was achieved with Zorbax SB-C18, 5.0µm (150\*4.6mm). The separation of Drug H was achieved by gradient elution using organic mixture (Acetonitrile: Methanol::80:20 v/v), buffer solution (10mM Ammonium Formate) and DMSO:: 50:50:0.2 v/v/v. The flow rate is 1.000 ml/min, Injection Volume of 10µl with a run time of 3.0 minutes. The calibration curve of standard Drug H was linear in range 0.1-20.0 ¿g/ml. 5 precision and accuracy batches were run; the coefficient of correlation (r2) was ¿ 0.99. The accuracy of the LLOQ in the standard CC was within ± 20% of the nominal concentration and ± 15% of the nominal concentration for other standards and 75% non- zero calibration standards met the acceptable criteria which indicated the method was precise and accurate. Method validation results have proved the method to be selective, sensitive, precise, accurate and robustness.VDM Verlag, Dudweiler Landstraße 99, 66123 Saarbrücken 128 pp. Englisch.…

Taschenbuch. Condizione: Neu. Bio-analytical method development of antimalarial drug in human plasma | Validation for quantitative estimation by using LC-MSMS | Bincy Abraham (u. a.) | Taschenbuch | Englisch | 2019 | LAP LAMBERT Academic Publishing | EAN 9786200482471 | Verantwortliche Person für die EU: preigu GmbH & Co. KG, Lengericher Landstr. 19, 49078 Osnabrück, mail[at]preigu[dot]de | Anbieter: preigu Print on Demand.…