Epithelial Transport: A guide to methods and experimental analysis - Rilegato

Wills, N. K.; Lewis, S. A.; Reuss, L.

 
9780412434006: Epithelial Transport: A guide to methods and experimental analysis

Sinossi

How is the epithelia organized? Which molecules mediate ion transport? How is an epithelia maintained? In this volume the authors discuss these and other key questions in the field and provide a clear guide to the methods used to answer them. The complexity of epithelial transport is reflected in the diverse approaches and methodologies used in its study. Written for active researchers, this book draws on the laboratory experience of leading investigators. An overview of the general principles underlying epithelial transport of ions, water and non-electrolytes is combined with guidance on practical approaches. The relative strengths and weaknesses of current methodologies are emphasized and potential pitfalls highlighted. This book should be of interest to graduates and researchers in cell biology, physiology, pharmacology, neurosciences and molecular biology.

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Recensione

...it will certainly attract not only the undergraduate and graduate students, but also post-doctoral fellows and established investigators. We think it will be particularly useful for clinicians and dermatologists... - Cellular and Molecular Biology

Contenuti

Epithelial structure and function. Epithelial transport proteins. Epithelial polarity. Characterization of epithelial ion transport. Epithelial electrophysiology. Impedance analysis of epithelia. Measurements and interpretation of cytoplasmic ion activities. Measurements of cell volume. Methods and experimental analysis of isolated epithelial membranes. Methods and experimental analysis of single ion channels. Epithelial cell culture. Measurements and interpretation of cytoplasmic ion activities. Signalling pathways regulating ion transport in polarized cells. The cytoskeleton and epithelial function. Future perspectives: molecular biology and pathophysiology.

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Altre edizioni note dello stesso titolo

9789401071680: Epithelial Transport: A guide to methods and experimental analysis

Edizione in evidenza

ISBN 10:  9401071683 ISBN 13:  9789401071680
Casa editrice: Springer, 2011
Brossura