Base Excision Repair Pathway: Methods and Protocols: 2701 - Brossura

Brossura

ISBN 10: 1071633759 ISBN 13: 9781071633755

Casa editrice: Humana, 2024

Riassunto

This detailed volume provides a comprehensive set of experimental protocols and useful strategies to examine the repair of damaged bases via the Base Excision Repair (BER) pathway in vitro and in cells. Beginning with multiple molecular and cellular techniques to examine the excision of damaged bases from double-stranded DNA or DNA wrapped in a nucleosome, the book continues with sections covering procedures to detect and quantify the damaged bases, protein DNA crosslinks, and double-strand breaks, experimental procedures to identify DNA repair protein interactome by conventional tandem affinity purification followed by mass spectroscopy analysis, as well as the analysis of genome-wide binding of DNA repair proteins and copy number variations of the DNA damage response gene in tumors. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Base Excision Repair Pathway: Methods and Protocols serves as a valuable resource for novices and experts trying to examine the repair of various types of DNA lesions in vitro and in cell by the distinct set of proteins in the BER pathway.

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Dalla quarta di copertina

This detailed volume provides a comprehensive set of experimental protocols and useful strategies to examine the repair of damaged bases via the Base Excision Repair (BER) pathway in vitro and in cells. Beginning with multiple molecular and cellular techniques to examine the excision of damaged bases from double-stranded DNA or DNA wrapped in a nucleosome, the book continues with sections covering procedures to detect and quantify the damaged bases, protein DNA crosslinks, and double-strand breaks, experimental procedures to identify DNA repair protein interactome by conventional tandem affinity purification followed by mass spectroscopy analysis, as well as the analysis of genome-wide binding of DNA repair proteins and copy number variations of the DNA damage response gene in tumors. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topis, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Base Excision Repair Pathway: Methods and Protocols serves as a valuable resource for novices and experts trying to examine the repair of various types of DNA lesions in vitro and in cell by the distinct set of proteins in the BER pathway.

Dettagli dell'edizione

• Editore: Humana
• Data di pubblicazione: 2024
• Lingua: Inglese
• ISBN 10: 1071633759
• ISBN 13: 9781071633755
• Rilegatura: Copertina flessibile
• Numero di pagine: 276
• Redattore: Bhakat Kishor K., Hazra Tapas K.
• Contatto del produttore: Springer Nature Customer Service Center GmbH; ProductSafety@springernature.com; ; Europaplatz 3,69115 Heidelberg, Germany; Heidelberg; 69115; Germania