Protein Interactions: Biophysical Approaches for the Study of Complex Reversible Systems: 5 - Brossura

Brossura

ISBN 10: 1441942084 ISBN 13: 9781441942081

Casa editrice: Springer Nature, 2011

Riassunto

This volume successfully and clearly examines how biophysical approaches can be used to study complex systems of reversibly interacting proteins. It deals with the methodology behind the research and shows how to synergistically incorporate several methodologies for use. Each chapter treats and introduces the reader to different biological systems, includes a brief summary of the physical principles, and mentions practical requirements.

Su questo libro

L'autore:

Dr. Peter Schuck is the acting chief of the Protein Biophysics department in the National Institute of Health’s Division of Bioengineering and Physical Science. His current research work is on protein structure and function, with studies in multi-protein complexes, membrane proteins in detergent solution, self-assembling and filamenting proteins, structural and nonstructural viral proteins, homogeneous and heterogeneous interactions of immunological cell surface receptors; and biophysical methods and instrumentation through analytical ultracentrifugation, optical biosensors, static and dynamic light scattering, calorimetry, and circular dichroism.

Contenuti:

The Characterization of Biomolecular Interactions Using Fluorescence Fluctuation Techniques.- Characterization of Protein–Protein Interactions Using Atomic Force Microscopy.- Combined Solid-Phase Detection Techniques for Dissecting Multiprotein Interactions on Membranes.- Surface Plasmon Resonance Biosensing in the Study of Ternary Systems of Interacting Proteins.- Mass Spectrometry for Studying Protein Modifications and for Discovery of Protein Interactions.- H/2H Exchange Mass Spectrometry of Protein Complexes.- Elucidation of Protein–Protein and Protein–Ligand Interactions by NMR Spectroscopy.- Application of Isothermal Titration Calorimetry in Exploring the Extended Interface.- Solvent Mediated Protein–Protein Interactions.- Sedimentation Equilibrium Analytical Ultracentrifugation for Multicomponent Protein Interactions.- Structure Analysis of Macromolecular Complexes by Solution Small-Angle Scattering.- Fluorescence Detection of Proximity.- Steady-State and Time-Resolved Emission Anisotropy.- Analysis of Protein-DNA Equilibria by Native Gel Electrophoresis.- Electrospray Ionization Mass Spectrometry and the Study of Protein Complexes.- Sedimentation Velocity in the Study of Reversible Multiprotein Complexes.

Su questa edizione

• Editore: Springer Nature
• Data di pubblicazione: 2011
• ISBN 10: 1441942084
• ISBN 13: 9781441942081
• Rilegatura: Copertina flessibile
• Numero di pagine: 544
• Redattore: Schuck Peter