Cohesin and Condensin + Ereference: Methods and Protocols: 1515 - Rilegato

Rilegato

ISBN 10: 1493965433 ISBN 13: 9781493965434

Casa editrice: Humana Pr Inc, 2016

Riassunto

The volume provides comprehensive, state-of-the-art experimental techniques that are now available to dissect the molecular mechanisms of regulation and function of cohesin and the related factor condensin in vitro and in vivo across different model organisms, as well as in human cells. Cohesin and Condensin: Methods and Protocols is divided into three parts: Part I explores various in vitro and in vivo systems used to study the fundamental mechanism of cohesin regulation in mitosis and meiosis; Part II summarizes experimental systems in a variety of organisms that are used to address interphase functions of cohesin and Nipbl in gene regulation and chromatin interaction, ribosome biogenesis and DNA repair, which contribute significantly to cohesion-associated disorders; Part III covers related condensin complex and describes techniques to study its role in mitosis and interphase. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Cohesin and Condensin: Methods and Protocols is a valuable resource for diverse audiences with interests in the relationship between chromatin organization and genomic functions.

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Dalla quarta di copertina

The volume provides comprehensive, state-of-the-art experimental techniques that are now available to dissect the molecular mechanisms of regulation and function of cohesin and the related factor condensin in vitro and in vivo across different model organisms, as well as in human cells. Cohesin and Condensin: Methods and Protocols is divided into three parts: Part I explores various in vitro and in vivo systems used to study the fundamental mechanism of cohesin regulation in mitosis and meiosis; Part II summarizes experimental systems in a variety of organisms that are used to address interphase functions of cohesin and Nipbl in gene regulation and chromatin interaction, ribosome biogenesis and DNA repair, which contribute significantly to cohesion-associated disorders; Part III covers related condensin complex and describes techniques to study its role in mitosis and interphase. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Cohesin and Condensin: Methods and Protocols is a valuable resource for diverse audiences with interests in the relationship between chromatin organization and genomic functions.

Dettagli dell'edizione

• Editore: Humana Pr Inc
• Data di pubblicazione: 2016
• Lingua: Inglese
• ISBN 10: 1493965433
• ISBN 13: 9781493965434
• Rilegatura: Copertina rigida
• Numero edizione: 1
• Numero di pagine: 288
• Redattore: Yokomori Kyoko, Shirahige Katsuhiko
• Contatto del produttore: ProductSafety@springernature.com; ProductSafety@springernature.com; ; Europaplatz 3; Heidelberg; 69115; Germania