Pyrosequencing Protocols: 373 - Brossura

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ISBN 10: 1617376930 ISBN 13: 9781617376931

Casa editrice: Humana, 2010

Riassunto

Pyrosequencing® Protocols presents detailed protocols for the multidisciplinary application of Pyrosequencing® technology, all written by world-renowned experts in the field. This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing®, and supplementing each protocol with troubleshooting tips specific to that method.

Chapters 1 and 2 present an introductory overview of the origins of the Pyrosequencing® methodology and its key applications. Chapters 3 and 4 describe primer selection and the basic Pyrosequencing® technique, and Chapters 5-7 provide methods for improving throughput and decreasing cost. Detailed applications for Pyrosequencing® comprise Chapters 8-13, and the important aspects of data storage is discussed in Chapter 14. This comprehensive volume both highlights the versatility of and provides detailed protocols for the application of Pyrosequencing®.

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Contenuti

Table of contents Chapter 1: The History of Pyrosequencing®. Pål Nyrén Chapter 2: Pyrosequencing® applications. Sharon Marsh Chapter 3: Web-Based Primer Design Software for Genome Scale Genotyping by Pyrosequencing®. Steven Ringquist, Christopher Pecoraro, Ying Lu, Alexis Styche, William A. Rudert, Panayiotis V. Benos and Massimo Trucco Chapter 4: Pyrosequencing®: A Simple Method for Accurate Genotyping. Cristi R. King and Tiffany Scott-Horton Chapter 5: Universal Primer Applications for Pyrosequencing®. Dong-chuan Guo and Dianna M. Milewicz Chapter 6: Allele Quantification and DNA Pooling Methods. Jonathon Wasson Chapter 7: Multiplex Pyrosequencing® for DNA Variation Analysis. Pritesh Patel, Yih-Horng Shiao, and Paolo Fortina Chapter 8: Analysis of gene-specific DNA methylation patterns by the Pyrosequencing® technology. Jörg Tost and Ivo Glynne Gut Chapter 9: Detecting mutations that confer oxazolidinone resistance in Gram-positive bacteria. Neil Woodford, Sarah E. North and Matthew J. Ellington Chapter 10: Pyrosequencing® Based Strategies for Improved Allele Typing of HLA Loci. Steven Ringquist, Alexis Styche, William A. Rudert, and Massimo Trucco Chapter 11: Pyrosequencing® of Phage Display Libraries for the Identification of Cell Specific Targeting Ligands. Ahad A. Rahim Chapter 12: Gene Copy Number Detection in Animal Studies. Gerli Pielberg and Leif Andersson Chapter 13: Detection of Allelic Imbalance in Gene Expression Using Pyrosequencing®. Hua Wang and Steven C. Elbein Chapter 14: Pyrosequencing® Genotype Storage Techniques. Derek J. Van Booven

Dettagli dell'edizione

• Editore: Humana
• Data di pubblicazione: 2010
• Lingua: Inglese
• ISBN 10: 1617376930
• ISBN 13: 9781617376931
• Rilegatura: Copertina flessibile
• Numero di pagine: 208
• Redattore: Marsh Sharon
• Contatto del produttore: non disponibile
• Persona responsabile: non disponibile