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During freezing, in addition to physical damage to membrane, crystallization can cause fatal damage to cells by pushing mobile molecules and confine them in small bits of cell volume causing dramatic decrease in local ion concentration eventually result in poisoning of cell organelles. Vitrification of intracellular molecules can overcome cryopreservation problems caused by crystallization. In order to achieve vitrification tremendous rates of heat removal must be applied on cells around 10^6 C/s, which are currently not possible for biological materials. In this research we investigated a new method via microfluidic devices proposed by Dr. Mehmet Toner for pre-concentration of cells with CPAs while putting cells under a lot fewer mechanical and osmotic stresses than traditional methods. New method is based on trapping cells into aqueous droplets and controlling the CPA concentration in the droplet by adjusting temperature of the system. By conducting Finite Element Analysis calculations of the microfluidic system, we found that it is possible to pre-concentrate mammalian cells with CPA 10 times the initial CPA concentration under 3 minutes with the new BioMEMS based method.
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L'autore
Throughout his undergraduate years, Ufuk Okumus completed an internship in University of Wisconsin-Madison, where he decided to pursue a Masters Degree.Worked on Cryopreservation and BioMEMS devices on his graduate years where he collaborated with BioMEMS Resource Center and Harvard Medical School.Currently working on Radiopharmaceuticals.
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Uniform Droplets in a Microfluidic System Designed for Biopreservation
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LAP Lambert Academic Publishing Dez 2016, 2016
ISBN 10: 333001203X
ISBN 13: 9783330012035
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Da: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germania
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Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -During freezing, in addition to physical damage to membrane, crystallization can cause fatal damage to cells by pushing mobile molecules and confine them in small bits of cell volume causing dramatic decrease in local ion concentration eventually result in poisoning of cell organelles. Vitrification of intracellular molecules can overcome cryopreservation problems caused by crystallization. In order to achieve vitrification tremendous rates of heat removal must be applied on cells around 10^6 C/s, which are currently not possible for biological materials. In this research we investigated a new method via microfluidic devices proposed by Dr. Mehmet Toner for pre-concentration of cells with CPAs while putting cells under a lot fewer mechanical and osmotic stresses than traditional methods. New method is based on trapping cells into aqueous droplets and controlling the CPA concentration in the droplet by adjusting temperature of the system. By conducting Finite Element Analysis calculations of the microfluidic system, we found that it is possible to pre-concentrate mammalian cells with CPA 10 times the initial CPA concentration under 3 minutes with the new BioMEMS based method. 88 pp. Englisch. Codice articolo 9783330012035
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Uniform Droplets in a Microfluidic System Designed for Biopreservation
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LAP LAMBERT Academic Publishing, 2016
ISBN 10: 333001203X
ISBN 13: 9783330012035
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Da: AHA-BUCH GmbH, Einbeck, Germania
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Taschenbuch. Condizione: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - During freezing, in addition to physical damage to membrane, crystallization can cause fatal damage to cells by pushing mobile molecules and confine them in small bits of cell volume causing dramatic decrease in local ion concentration eventually result in poisoning of cell organelles. Vitrification of intracellular molecules can overcome cryopreservation problems caused by crystallization. In order to achieve vitrification tremendous rates of heat removal must be applied on cells around 10^6 C/s, which are currently not possible for biological materials. In this research we investigated a new method via microfluidic devices proposed by Dr. Mehmet Toner for pre-concentration of cells with CPAs while putting cells under a lot fewer mechanical and osmotic stresses than traditional methods. New method is based on trapping cells into aqueous droplets and controlling the CPA concentration in the droplet by adjusting temperature of the system. By conducting Finite Element Analysis calculations of the microfluidic system, we found that it is possible to pre-concentrate mammalian cells with CPA 10 times the initial CPA concentration under 3 minutes with the new BioMEMS based method. Codice articolo 9783330012035
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Uniform Droplets in a Microfluidic System Designed for Biopreservation
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LAP LAMBERT Academic Publishing, 2016
ISBN 10: 333001203X
ISBN 13: 9783330012035
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Da: Revaluation Books, Exeter, Regno Unito
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Paperback. Condizione: Brand New. 88 pages. 8.66x5.91x0.20 inches. In Stock. Codice articolo 333001203X
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Uniform Droplets in a Microfluidic System Designed for Biopreservation
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LAP LAMBERT Academic Publishing, 2016
ISBN 10: 333001203X
ISBN 13: 9783330012035
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Da: moluna, Greven, Germania
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Condizione: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: Okumus UfukThroughout his undergraduate years, Ufuk Okumus completed an internship in University of Wisconsin-Madison, where he decided to pursue a Masters Degree.Worked on Cryopreservation and BioMEMS devices on his graduate years w. Codice articolo 158421731
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Uniform Droplets in a Microfluidic System Designed for Biopreservation
Editore:
LAP LAMBERT Academic Publishing Dez 2016, 2016
ISBN 10: 333001203X
ISBN 13: 9783330012035
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Da: buchversandmimpf2000, Emtmannsberg, BAYE, Germania
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Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -During freezing, in addition to physical damage to membrane, crystallization can cause fatal damage to cells by pushing mobile molecules and confine them in small bits of cell volume causing dramatic decrease in local ion concentration eventually result in poisoning of cell organelles. Vitrification of intracellular molecules can overcome cryopreservation problems caused by crystallization. In order to achieve vitrification tremendous rates of heat removal must be applied on cells around 10^6 C/s, which are currently not possible for biological materials. In this research we investigated a new method via microfluidic devices proposed by Dr. Mehmet Toner for pre-concentration of cells with CPAs while putting cells under a lot fewer mechanical and osmotic stresses than traditional methods. New method is based on trapping cells into aqueous droplets and controlling the CPA concentration in the droplet by adjusting temperature of the system. By conducting Finite Element Analysis calculations of the microfluidic system, we found that it is possible to pre-concentrate mammalian cells with CPA 10 times the initial CPA concentration under 3 minutes with the new BioMEMS based method.VDM Verlag, Dudweiler Landstraße 99, 66123 Saarbrücken 88 pp. Englisch. Codice articolo 9783330012035
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Uniform Droplets in a Microfluidic System Designed for Biopreservation
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LAP LAMBERT Academic Publishing, 2016
ISBN 10: 333001203X
ISBN 13: 9783330012035
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Da: preigu, Osnabrück, Germania
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Taschenbuch. Condizione: Neu. Uniform Droplets in a Microfluidic System Designed for Biopreservation | Ufuk Okumus | Taschenbuch | 88 S. | Englisch | 2016 | LAP LAMBERT Academic Publishing | EAN 9783330012035 | Verantwortliche Person für die EU: preigu GmbH & Co. KG, Lengericher Landstr. 19, 49078 Osnabrück, mail[at]preigu[dot]de | Anbieter: preigu. Codice articolo 107999013
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