Articoli correlati a Method Development and Validation for Voriconazole...

Method Development and Validation for Voriconazole Estimation: RP-HPLC and UV Spectrophotometric Methods in Bulk and Pharmaceutical Formulations - Brossura

Srinivasa Rao, Yarguntla; Vara Prasada Rao, K.

 
9783330051331: Method Development and Validation for Voriconazole Estimation: RP-HPLC and UV Spectrophotometric Methods in Bulk and Pharmaceutical Formulations

Sinossi

A rapid, sensitive, and specific RP-HPLC method with UV detection was developed and validated for the determination of voriconazole. Chromatography was performed on an Enable C18 G column (250 × 4.6 mm) in isocratic mode, using a mobile phase of methanol and acetonitrile (60:40, v/v). The flow rate was set at 1 mL/min, and the effluent was monitored at 223 nm. Chromatographic analysis revealed a main peak for voriconazole at a retention time of 2.841 minutes. Methanol was selected as the solvent for the analysis, with the λmax determined to be 223 nm. The method exhibited linearity over a concentration range of 5–30 µg/mL. Validation parameters, including linearity, range, precision, accuracy, limit of detection (LOD), and limit of quantitation (LOQ), were assessed and met the required standards.

Le informazioni nella sezione "Riassunto" possono far riferimento a edizioni diverse di questo titolo.