Sinossi
Designed as an introductory text the authors cover all core strategies in the application of modern recombinant DNA technology. The first chapters directly address the applications of polymerase chain reaction to a variety of problems in DNA cloning that are, or have been, extremely challenging using more traditional approaches and technologies. These include cDNA cloning and transcript mapping, mutagenesis as well as the cloning of very long transcripts and protocols using limiting amounts of total RNA. Further chapters describe approaches to subtractive cloning technologies as well as novel specialized expression cloning and library screening strategies. The handbook contains detailed step-by-step protocols and extensive hands-on advice.
Le informazioni nella sezione "Riassunto" possono far riferimento a edizioni diverse di questo titolo.
Contenuti
PCR and cDNA Cloning Techniques.- 1 Strategies for cDNA Cling and Mapping RNA Transcripts.- 2 Cloning PCR Products.- 3 Site Directed Deletion, Insertion, and Substitution Using PCR.- 4 Long RT-PCR Cloning — Amplification of Full-Length Enterovirus Genomes.- 5 Construction of cDNA Libraries from Small Quantities of Total RNA Using Template Switching Catalyzed by M-MLV Reverse Transcriptase.- Subtractive and Differential Display Techniques.- 6 Subtractive Hybridization and cDNA Cloning.- 7 Differential Display to Identify Steroid-Induced Genes in Endocrine Cells.- Specialized Cloning and Library Screening Strategies.- 8 Two Hyrid cDNA Cloning.- 9 Yeast One and Two Hyrid cDNA Cloning.- 10 High-Throughput Library Screening by Fluorescent Hybridisations on Gridded Membranes.- 11 Identification of Cell Targeting Ligands Using Random Peptide-Presenting Phage Libraries.
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Genetic Library Construction and Screening
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Condizione: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. A comprehensive, up-to-date guide on all aspects of synthesis and screening of genetic librariesDetailed step-by-step protocols and extensive hands-on advice gleaned from the authors experiencePerfectly suited for experienced researchers and gradua. Codice articolo 5062205
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ISBN 10: 364247733X
ISBN 13: 9783642477331
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Genetic Library Construction and Screening : Advanced Techniques and Applications
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Springer Berlin Heidelberg, 2012
ISBN 10: 364247733X
ISBN 13: 9783642477331
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Da: AHA-BUCH GmbH, Einbeck, Germania
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