Articoli correlati a Applications of Continuous and Steady-State Methods...

Applications of Continuous and Steady-State Methods to Root Biology: 34 - Brossura

 
9789401075022: Applications of Continuous and Steady-State Methods to Root Biology: 34
Brossura
ISBN 10: 9401075026 ISBN 13: 9789401075022
Casa editrice: Springer, 2011

Sinossi

This book provides an excellent illustration of the interrelationship between progress in scientific methodology and conceptual advances, and its publica­ tion should contribute to further advances. It is well known that major advances in understanding often follow the development of new methods. The development of the acetylene reduction assay for nitrogenase activity provides a good example of this interrelationship between theory and methods. Theoretical knowledge led to a search for substrates for nitro­ genase that could be assayed for more easily than ammonium, the normal product of the enzyme. The discovery of the reduction of acetylene to ethylene by nitrogenase provided the ideal answer to the problem by provid­ ing a rapid, specific, nondestructive, and inexpensive assay for nitrogenase activity. This assay is now used by almost every laboratory doing research on nitrogen fixation. However, further use and development of the acetylene reduction assay has shown that it can underestimate nitrogenase activity and can even give incorrect relative values under some circumstances. The major problem is that exposure of legume nodules to acetylene can cause a large increase in the resistance to oxygen diffusion into the nodule. This reduced supply of oxygen decreases the rate of nitrogenase activity within a few minutes.

Le informazioni nella sezione "Riassunto" possono far riferimento a edizioni diverse di questo titolo.

Dalla quarta di copertina

This book provides an excellent illustration of the interrelationship between progress in scientific methodology and conceptual advances, and its publica­ tion should contribute to further advances. It is well known that major advances in understanding often follow the development of new methods. The development of the acetylene reduction assay for nitrogenase activity provides a good example of this interrelationship between theory and methods. Theoretical knowledge led to a search for substrates for nitro­ genase that could be assayed for more easily than ammonium, the normal product of the enzyme. The discovery of the reduction of acetylene to ethylene by nitrogenase provided the ideal answer to the problem by provid­ ing a rapid, specific, nondestructive, and inexpensive assay for nitrogenase activity. This assay is now used by almost every laboratory doing research on nitrogen fixation. However, further use and development of the acetylene reduction assay has shown that it can underestimate nitrogenase activity and can even give incorrect relative values under some circumstances. The major problem is that exposure of legume nodules to acetylene can cause a large increase in the resistance to oxygen diffusion into the nodule. This reduced supply of oxygen decreases the rate of nitrogenase activity within a few minutes.

Le informazioni nella sezione "Su questo libro" possono far riferimento a edizioni diverse di questo titolo.

Editore
Springer
Data di pubblicazione
2011
Lingua
Inglese
ISBN 10 
9401075026
ISBN 13 
9789401075022
Rilegatura
Copertina flessibile
Numero di pagine
248
Redattore
Torrey John G., Winship Lawrence J.
Contatto del produttore
Springer Nature Customer Service Center GmbH
ProductSafety@springernature.com

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