Bioengineering at the micro-scale (Band 81)

Lingua: inglese

Editore: Cuvillier Mai 2019, 2019

3736970250 / 9783736970250

Da: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, GermaniaBuchWeltWeit Ludwig Meier e.K.

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Venditore AbeBooks dal 11 gennaio 2012

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This item is printed on demand - it takes 3-4 days longer - Neuware -The miniaturisation of bioreactors to the microliter-scale and the integration of online sensors for monitoring the most important process variables during the cultivations is a promising approach for the screening and optimization of cultivation and biocatalysis processes, enabling information-rich, parallelised and cost-effective experiments under well-controlled environmental conditions. The main advantages of microbioreactors (MBRs) are minimization of space and reagents, their easy manipulation, and their high-throughput screening potential, which make them very interesting tools to develop bioprocesses.This thesis is focused on the development of MBRs and the sensor integration for monitoring optical density (OD), dissolved oxygen (DO), pH and glucose as well as their validation for different biotechnological applications. On the basis of a developed PDMS-based MBR, a borosilicate glass-based microbubble column-biroeactor (g¿BC) (working volume of 60 ¿L, aeration occurs through a nozzle with Ø =26 ¿m) was designed and manufactured by wet etching and powder blasting technology. The gµBC proved to have good oxygen transfer capacity, reaching kLa values up to 320 1/h and fast mixing times ¿95 down to 5.5 s when working at a gas superficial velocity uG of 2.25¿10-3 m/s. The mixing performance was simulated using a simplified CFD model, and the tracer profile yielded a good qualitative prediction that was comparable to the experimental results, presenting a tolerable deviation of the mixing times. The gµBC was validated as a suitable cultivation screening tool with a batch cultivation of Saccharomyces cerevisiae with the real-time online monitoring of OD and DO. Furthermore, a cuvette-based microbubble column-bioreactor (c¿BC) made of polystyrene (working volume of 550 ¿L, aeration occurs through a nozzle with Ø ¿ 100 ¿m) was developed and manufactured with online sensors for pH, OD, DO and glucose. The c¿BC showed homogeneous mixing of the cultivation medium with ¿95 138 pp. Englisch.…

Codice articolo 9783736970250

Titolo
Bioengineering at the micro-scale (Band 81)
Autore
Susanna Maria Lladó Maldonado
Editore
Cuvillier Mai 2019
Anno di pubblicazione
2019
Condizione
Neu
Rilegatura
Taschenbuch
Lingua
inglese
ISBN 10
3736970250
ISBN 13
9783736970250
Peso dell'articolo
189 grammi
Dimensioni
210x148x8 mm

BuchWeltWeit Ludwig Meier e.K.

Bergisch Gladbach, Germania

Venditore con 5 stelle

Venditore AbeBooks dal 11 gennaio 2012

Tariffe di spedizione da Germania a U.S.A.

ArticoloDa 5 a 15 giorni lavorativiDa 5 a 15 giorni lavorativi
Primo articoloEUR 23,00EUR 23,00
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BuchWeltWeit Ludwig Meier e.K.

Germania